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Current IssueEditorial BoardOnline First ArticlesArchive

volume 46 issue 1 (march 2012) : 82 - 85

IN VITRO ANTIPASTEURELLOSIS ACTIVITY OF DIFFERENT EXTRACTS OF POLYALTHIA LONGIFOLIA LEAVES

N
N.P. Usha*
B
Bibu John
P
P. Subin Jose.
K
K.M. Jayakumar
A
A.M.C. Nair1
G
G. Krishnan Nair2
N
N.D. Nair3
1College of Veterinary and Animal Sciences, Mannuthy, Thrissur – 680 651, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Usha* N.P., John Bibu, Jose. Subin P., Jayakumar K.M., Nair1 A.M.C., Nair2 Krishnan G., Nair3 N.D. (2025). IN VITRO ANTIPASTEURELLOSIS ACTIVITY OF DIFFERENT EXTRACTS OF POLYALTHIA LONGIFOLIA LEAVES . Indian Journal of Animal Research. 46(1): 82 - 85. doi: .

ABSTRACT

The outbreaks of duck pasteurellosis which occur during monsoon period, caused by avian strains of Pasteurella multocida, is a serious problem with high mortality and morbidity affecting younger age groups. Polyalthia longifolia (P. longifolia ) leaves which had antibacterial action, was tested for its efficacy against P. multocida by in vitro methods. The successive extracts of the plant materials were tested for its antibacterial effect using microtitre plate technique (to estimate the minimum inhibitory concentration, MIC) and disc diffusion method was performed to estimate the zone of inhibition. Microtitre plate technique was assayed by dissolving the plant extracts in sterile DMSO except the aqueous extracts which were dissolved in sterile distilled water. Various concentrations, 200 ìg, 500 ìg and 1 mg/well, of successive extracts of P. longifolia were used to find out the MIC. Disc diffusion method was performed with successive extracts of P. longifolia leaves at 200 µg, 500 µg and 1 mg concentrations. The results from the above in vitro assay revealed the efficacy of acetonic extract of P. longifolia leaves against duck pasteurellosis. The acetonic extract was further subjected to spectrophotometric, thin layer chromatography and high performance thin layer chromatographic analysis. The results of the present study could describe the antipasteurellosis activity of P. longifolia leaves.

REFERENCES

  1. Barrow, C. I. and Feltham, R. K. A. (1993). Cowan and Steel’s Manual for Identification of Medical Bacteria. Cambridge University Press, 331p.
  2. Batista, O., Duarte, A., Nascimento, J. and Simoes, M.F. (1994). Structure and antimicrobial activity of diterpenes from the roots of Plectranthus hereroensis, J. Nat. Prod., 57: 858–861.
  3. Bauer, A.W., Kirby, W.M., Sherris, J.C. and Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disk method, Am. J. Clin. Pathol., 45:493-496.
  4. Buxton, A. and Fraser, G. (1977). Animal Microbiology. Blackwell Scientific Publication, Oxford, 92-102.
  5. Daferera, D.J., Ziogas, B.N. and Polissiou, M.G. (2003). The effectiveness of plant essential oils on the growth of Botrytis cinerea, Fusarium sp. and Clavibacter michiganensis subsp. michiganensis, Crop Prot., 22:39–44.
  6. Devi, V. R., Rao, T. S., Aruna, P. and Sujani, G. (2000). Incidence of duck cholera in Krishna district, Andhra Pradesh – a field report, Indian Vet. J., 79: 535-536.
  7. Faizi, S., Mughal, N.R., Khan, R.A., Khan, S.A., Ahmad,A., Bibi, N. and Ahmed, S.A. (2003). Evaluation of the antimicrobial property of Polyalthia longifolia var. pendula: isolation of a lactone as the active antibacterial agent from the ethanol extract of the stem, Phytother. Res., 17: 1177-1181.
  8. Ghosh, A., Das, B.K., Chatterjee, S.K. and Chandra, G. (2008). Antibacterial potentiality and phytochemical analysis of mature leaves of Polyalthia longifolia (Magnoliales: Annonaceae), South Pacific J. Natural Sci., 26:68-72.
  9. Harborne, J.B. (1991). Phytochemical Methods-Guide to Modern Techniques of Plant Analysis. 2nd Edn., Chapman and Hall, India, 653p.
  10. Heddleston, K. L. (1976). Physiologic characteristics of 1,268 cultures of Pasteurella multocida, Am. J. Vet. Res., 37: 745-747.
  11. Kazmi, M.H., Malik, A., Hameed, S., Akhtar, N. and Ali,S.N. (1994). An anthraquinone derivative from Cassia italica Phytochemistry, 36: 761–763.
  12. Murthy, M.M., Subramanyam, M., Bindu, M.H. and Annapurna, J. (2005). Antimicrobial activity of clerodane diterpenoids from Polyalthia longifolia seeds Fitoterapia, 76 (3-4): 336-339.
  13. Nair R. and Chanda S. (2006). Evaluation of Polyalthia longifolia (Sonn.), Thw. leaf extract for antifungal activity, J. Cell Tissue Res., 581-584.
  14. Nair, R., Kalariya, T. and Chanda, S. (2005). Antibacterial activity of some selected Indian medicinal flora Turk. J. Biol., 29: 41-47.
  15. Omulokoli, E., Khan, B. and Chhabra, S.C. (1997). Antiplasmodial activity of four Kenyan medicinal plants, J. Ethnopharmacol., 56: 133–137.
  16. Sheena, N., Ajith, T.A., Mathew, T. and Janardhanam, K.K. (2003). Pharm. Biol., 41:564-567.
  17. Shivpuri A, Sharma OP, Jhamaria SL. (1997). Fungitoxic properties of plant extracts against pathogenic fungi, J. Mycol. Plant Pathol., 27: 29-31.
  18. Srivastava, M. (2011). High-performance Thin-layer Chromatography (HPTLC). Springer, New York. 203-222.
  19. Taylor, R.S.L., Edel, F., Manandhar, N.P. and Towers,G.H.N. (1996). Antimicrobial activities of southern Nepalese medicinal plants, J. Ethnopharmacol., 50: 97–102.
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    volume 46 issue 1 (march 2012) : 82 - 85

    IN VITRO ANTIPASTEURELLOSIS ACTIVITY OF DIFFERENT EXTRACTS OF POLYALTHIA LONGIFOLIA LEAVES

    N
    N.P. Usha*
    B
    Bibu John
    P
    P. Subin Jose.
    K
    K.M. Jayakumar
    A
    A.M.C. Nair1
    G
    G. Krishnan Nair2
    N
    N.D. Nair3
    1College of Veterinary and Animal Sciences, Mannuthy, Thrissur – 680 651, India

    • Submitted|

    • First Online |

    • doi

    Cite article:- Usha* N.P., John Bibu, Jose. Subin P., Jayakumar K.M., Nair1 A.M.C., Nair2 Krishnan G., Nair3 N.D. (2025). IN VITRO ANTIPASTEURELLOSIS ACTIVITY OF DIFFERENT EXTRACTS OF POLYALTHIA LONGIFOLIA LEAVES . Indian Journal of Animal Research. 46(1): 82 - 85. doi: .

    ABSTRACT

    The outbreaks of duck pasteurellosis which occur during monsoon period, caused by avian strains of Pasteurella multocida, is a serious problem with high mortality and morbidity affecting younger age groups. Polyalthia longifolia (P. longifolia ) leaves which had antibacterial action, was tested for its efficacy against P. multocida by in vitro methods. The successive extracts of the plant materials were tested for its antibacterial effect using microtitre plate technique (to estimate the minimum inhibitory concentration, MIC) and disc diffusion method was performed to estimate the zone of inhibition. Microtitre plate technique was assayed by dissolving the plant extracts in sterile DMSO except the aqueous extracts which were dissolved in sterile distilled water. Various concentrations, 200 ìg, 500 ìg and 1 mg/well, of successive extracts of P. longifolia were used to find out the MIC. Disc diffusion method was performed with successive extracts of P. longifolia leaves at 200 µg, 500 µg and 1 mg concentrations. The results from the above in vitro assay revealed the efficacy of acetonic extract of P. longifolia leaves against duck pasteurellosis. The acetonic extract was further subjected to spectrophotometric, thin layer chromatography and high performance thin layer chromatographic analysis. The results of the present study could describe the antipasteurellosis activity of P. longifolia leaves.

    REFERENCES

    1. Barrow, C. I. and Feltham, R. K. A. (1993). Cowan and Steel’s Manual for Identification of Medical Bacteria. Cambridge University Press, 331p.
    2. Batista, O., Duarte, A., Nascimento, J. and Simoes, M.F. (1994). Structure and antimicrobial activity of diterpenes from the roots of Plectranthus hereroensis, J. Nat. Prod., 57: 858–861.
    3. Bauer, A.W., Kirby, W.M., Sherris, J.C. and Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disk method, Am. J. Clin. Pathol., 45:493-496.
    4. Buxton, A. and Fraser, G. (1977). Animal Microbiology. Blackwell Scientific Publication, Oxford, 92-102.
    5. Daferera, D.J., Ziogas, B.N. and Polissiou, M.G. (2003). The effectiveness of plant essential oils on the growth of Botrytis cinerea, Fusarium sp. and Clavibacter michiganensis subsp. michiganensis, Crop Prot., 22:39–44.
    6. Devi, V. R., Rao, T. S., Aruna, P. and Sujani, G. (2000). Incidence of duck cholera in Krishna district, Andhra Pradesh – a field report, Indian Vet. J., 79: 535-536.
    7. Faizi, S., Mughal, N.R., Khan, R.A., Khan, S.A., Ahmad,A., Bibi, N. and Ahmed, S.A. (2003). Evaluation of the antimicrobial property of Polyalthia longifolia var. pendula: isolation of a lactone as the active antibacterial agent from the ethanol extract of the stem, Phytother. Res., 17: 1177-1181.
    8. Ghosh, A., Das, B.K., Chatterjee, S.K. and Chandra, G. (2008). Antibacterial potentiality and phytochemical analysis of mature leaves of Polyalthia longifolia (Magnoliales: Annonaceae), South Pacific J. Natural Sci., 26:68-72.
    9. Harborne, J.B. (1991). Phytochemical Methods-Guide to Modern Techniques of Plant Analysis. 2nd Edn., Chapman and Hall, India, 653p.
    10. Heddleston, K. L. (1976). Physiologic characteristics of 1,268 cultures of Pasteurella multocida, Am. J. Vet. Res., 37: 745-747.
    11. Kazmi, M.H., Malik, A., Hameed, S., Akhtar, N. and Ali,S.N. (1994). An anthraquinone derivative from Cassia italica Phytochemistry, 36: 761–763.
    12. Murthy, M.M., Subramanyam, M., Bindu, M.H. and Annapurna, J. (2005). Antimicrobial activity of clerodane diterpenoids from Polyalthia longifolia seeds Fitoterapia, 76 (3-4): 336-339.
    13. Nair R. and Chanda S. (2006). Evaluation of Polyalthia longifolia (Sonn.), Thw. leaf extract for antifungal activity, J. Cell Tissue Res., 581-584.
    14. Nair, R., Kalariya, T. and Chanda, S. (2005). Antibacterial activity of some selected Indian medicinal flora Turk. J. Biol., 29: 41-47.
    15. Omulokoli, E., Khan, B. and Chhabra, S.C. (1997). Antiplasmodial activity of four Kenyan medicinal plants, J. Ethnopharmacol., 56: 133–137.
    16. Sheena, N., Ajith, T.A., Mathew, T. and Janardhanam, K.K. (2003). Pharm. Biol., 41:564-567.
    17. Shivpuri A, Sharma OP, Jhamaria SL. (1997). Fungitoxic properties of plant extracts against pathogenic fungi, J. Mycol. Plant Pathol., 27: 29-31.
    18. Srivastava, M. (2011). High-performance Thin-layer Chromatography (HPTLC). Springer, New York. 203-222.
    19. Taylor, R.S.L., Edel, F., Manandhar, N.P. and Towers,G.H.N. (1996). Antimicrobial activities of southern Nepalese medicinal plants, J. Ethnopharmacol., 50: 97–102.
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