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Current IssueEditorial BoardOnline First ArticlesArchive

volume 46 issue 1 (march 2012) : 61 - 65

EXPRESSION AND IMMUNOHISTOCHEMICAL LOCALIZATION OF GHRELIN GENE IN BUFFALO (BUBALUS BUBALIS) CORPUS LUTEUM

A
Asit Jain1
T
Tripti Jain2
A
Abhijit Mitra
1Genome Analysis Laboratory, Division of Animal Genetics Indian Veterinary Research Institute, Izatnagar, Bareilly- 243 122, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Jain1 Asit, Jain2 Tripti, Mitra Abhijit (2025). EXPRESSION AND IMMUNOHISTOCHEMICAL LOCALIZATION OF GHRELIN GENE IN BUFFALO (BUBALUS BUBALIS) CORPUS LUTEUM. Indian Journal of Animal Research. 46(1): 61 - 65. doi: .

ABSTRACT

Ghrelin, a novel motilin related 28-amino acid peptide, is an endogenous ligand for GH secretagogue receptor (GHS-R). In addition to its neuro-endocrine effects in the control of growth hormone (GH) secretion and food intake, recently an unexpected reproductive facet of ghrelin has emerged as its expression and cognate receptors are reported in the rat testis. However, the presence of this signalling system is yet to be ascertained in the buffalo gonads.  In this study, amplified 230 bp of ghrelin gene from buffalo corpus luteum (CL) using cDNA as a template have been taken.  Also, by immunohistochemistry using specific polyclonal antibodies, the presence and localization of ghrelin in the cyclic and pregnant CL were assessed. Expression of ghrelin protein was persistent local observed in the estrous cycle and early pregnancy (~d40). However, the signals of ghrelin protein were found to be higher during early pregnancy compared to estrous cycle. These dynamic changes in the expression profile of ghrelin during the estrous cycle and throughout the pregnancy propose a precise regulation of ovarian expression of ghrelin, which could portray the potential role of ghrelin in regulation of luteal development. In conclusion our study is the first to demonstrate the expression of ghrelin in the cyclic and pregnant buffalo CL which opens up the possibility of using this molecule in regulating the reproduction.

REFERENCES

  1. Ariyasu, H., Takaya K., Tagami T., Ogawa Y., Hosoda K., Akamizu T., Suda M., Koh T., Natsui K., Toyooka S., Shirakami G., Usui T., Shimatsu A., Doi K., Hosoda H., Kojima M., Kangawa K. and Nakao K. (2001). Stomach is a major source of circulating ghrelin, and feeding state determines plasma ghrelin-like immunoreactivity levels in humans. J Clin Endocrinol Metab., 86:4753-4758.
  2. Arvat, E., Maccario M., Di Vito L., Broglio F., Benso A., Gottero C., Papotti M., Muccioli G., Dieguez C., Casanueva F.F., Deghenghi R., Camanni F. and Ghigo E. (2001). Endocrine activities of ghrelin, a natural growth hormone secretagogue (ghs), in humans: Comparison and interactions with hexarelin, a nonnatural peptidyl ghs, and gh- releasing hormone. J Clin Endocrinol Metab., 86:1169-1174.
  3. Barreiro, M.L., Tena-Sempere M. (2004). Ghrelin and reproduction: A novel signal linking energy status and fertility? Mol Cell Endocrinol., 226:1-9.
  4. Broglio, F., Arvat E., Benso A., Gottero C., Muccioli G., Papotti M., van der Lely A.J., Deghenghi R., Ghigo E. (2001). Ghrelin, a natural gh secretagogue produced by the stomach, induces hyperglycemia and reduces insulin secretion in humans. J Clin Endocrinol Metab., 86:5083-5086.
  5. Caminos, J.E., Tena-Sempere M., Gaytan F. ( 2003) Expression of ghrelin in the cyclic and pregnant rat ovary . Endocrinology, 144:1594–602.
  6. Campanile, G., Di Palo R, Neglia G., Vecchio D., Gasparrini B., Prandi A., Galiero G., D’Occhio M.J. (2007) Corpus luteum function and embryonic mortality in buffaloes treated with a gnrh agonist, hcg and progesterone. Theriogenology, 67:1393-1398.
  7. Campanile, G., et al., (2005). Theriogenology, 63:2334-2340.
  8. Date, Y., Kojima M., Hosoda H., Sawaguchi A., Mondal M.S., Suganuma T., Matsukura S., Kangawa K., Nakazato M. (2000) Ghrelin, a novel growth hormone-releasing acylated peptide, is synthesized in a distinct endocrine cell type in the gastrointestinal tracts of rats and humans. Endocrinology, 141:4255-4261.
  9. Du C., Xilingaowa, Cao, G., Wang, C., Li, H., Zhao, Y., Siqingaowa, Cao, J. (2009) Expression of the orexigenic peptide ghrelin in the sheep ovary. Domestic Animal Endocrinology, 36 :89–98.
  10. Duggal P.S., Weitsman S.R., Magofûn D.A. and Norman R.J. (2002) Expression of the long (OB-RB) and short (OB-RA) forms of the leptin receptor throughout the oestrous cycle in the mature rat ovary. Reproduction, 123: 899–905.
  11. Duxbury, M.S., Waseem, T., Ito, H., Robinson, M.K., Zinner, M.J., Ashley, S.W. (2003) Ghrelin promotes pancreatic adenocarcinoma cellular proliferation and invasiveness. Biochem Biophys Res Commun., 309:468.
  12. Evans H.E., Sack W.O. (1973) Prenatal development of domestic and laboratory mammals: growth curves, external features and selected references. Zentralbl Veterinarmed., 2(1):11-45.
  13. FAO, 2008, http://www.fao.org.
  14. Garcia, M.C., Lopez M., Alvarez C.V., Casanueva F., Tena-Sempere M., Dieguez C. (2007) Role of ghrelin in reproduction. Reproduction, 133:531–540.
  15. Gaytan, F., Barreiro, M. L., Chopin, L. K. (2003). “Immunolocalization of ghrelin and its functional receptor, the type 1a growth hormone secretagogue receptor, in the cyclic human ovary. J Clin Endocrinol Metab., 88: 879–887.
  16. Gualillo, O., et al., (2003). FEBS Lett., 552:105–109.
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Indian Journal of Animal Research
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    volume 46 issue 1 (march 2012) : 61 - 65

    EXPRESSION AND IMMUNOHISTOCHEMICAL LOCALIZATION OF GHRELIN GENE IN BUFFALO (BUBALUS BUBALIS) CORPUS LUTEUM

    A
    Asit Jain1
    T
    Tripti Jain2
    A
    Abhijit Mitra
    1Genome Analysis Laboratory, Division of Animal Genetics Indian Veterinary Research Institute, Izatnagar, Bareilly- 243 122, India

    • Submitted|

    • First Online |

    • doi

    Cite article:- Jain1 Asit, Jain2 Tripti, Mitra Abhijit (2025). EXPRESSION AND IMMUNOHISTOCHEMICAL LOCALIZATION OF GHRELIN GENE IN BUFFALO (BUBALUS BUBALIS) CORPUS LUTEUM. Indian Journal of Animal Research. 46(1): 61 - 65. doi: .

    ABSTRACT

    Ghrelin, a novel motilin related 28-amino acid peptide, is an endogenous ligand for GH secretagogue receptor (GHS-R). In addition to its neuro-endocrine effects in the control of growth hormone (GH) secretion and food intake, recently an unexpected reproductive facet of ghrelin has emerged as its expression and cognate receptors are reported in the rat testis. However, the presence of this signalling system is yet to be ascertained in the buffalo gonads.  In this study, amplified 230 bp of ghrelin gene from buffalo corpus luteum (CL) using cDNA as a template have been taken.  Also, by immunohistochemistry using specific polyclonal antibodies, the presence and localization of ghrelin in the cyclic and pregnant CL were assessed. Expression of ghrelin protein was persistent local observed in the estrous cycle and early pregnancy (~d40). However, the signals of ghrelin protein were found to be higher during early pregnancy compared to estrous cycle. These dynamic changes in the expression profile of ghrelin during the estrous cycle and throughout the pregnancy propose a precise regulation of ovarian expression of ghrelin, which could portray the potential role of ghrelin in regulation of luteal development. In conclusion our study is the first to demonstrate the expression of ghrelin in the cyclic and pregnant buffalo CL which opens up the possibility of using this molecule in regulating the reproduction.

    REFERENCES

    1. Ariyasu, H., Takaya K., Tagami T., Ogawa Y., Hosoda K., Akamizu T., Suda M., Koh T., Natsui K., Toyooka S., Shirakami G., Usui T., Shimatsu A., Doi K., Hosoda H., Kojima M., Kangawa K. and Nakao K. (2001). Stomach is a major source of circulating ghrelin, and feeding state determines plasma ghrelin-like immunoreactivity levels in humans. J Clin Endocrinol Metab., 86:4753-4758.
    2. Arvat, E., Maccario M., Di Vito L., Broglio F., Benso A., Gottero C., Papotti M., Muccioli G., Dieguez C., Casanueva F.F., Deghenghi R., Camanni F. and Ghigo E. (2001). Endocrine activities of ghrelin, a natural growth hormone secretagogue (ghs), in humans: Comparison and interactions with hexarelin, a nonnatural peptidyl ghs, and gh- releasing hormone. J Clin Endocrinol Metab., 86:1169-1174.
    3. Barreiro, M.L., Tena-Sempere M. (2004). Ghrelin and reproduction: A novel signal linking energy status and fertility? Mol Cell Endocrinol., 226:1-9.
    4. Broglio, F., Arvat E., Benso A., Gottero C., Muccioli G., Papotti M., van der Lely A.J., Deghenghi R., Ghigo E. (2001). Ghrelin, a natural gh secretagogue produced by the stomach, induces hyperglycemia and reduces insulin secretion in humans. J Clin Endocrinol Metab., 86:5083-5086.
    5. Caminos, J.E., Tena-Sempere M., Gaytan F. ( 2003) Expression of ghrelin in the cyclic and pregnant rat ovary . Endocrinology, 144:1594–602.
    6. Campanile, G., Di Palo R, Neglia G., Vecchio D., Gasparrini B., Prandi A., Galiero G., D’Occhio M.J. (2007) Corpus luteum function and embryonic mortality in buffaloes treated with a gnrh agonist, hcg and progesterone. Theriogenology, 67:1393-1398.
    7. Campanile, G., et al., (2005). Theriogenology, 63:2334-2340.
    8. Date, Y., Kojima M., Hosoda H., Sawaguchi A., Mondal M.S., Suganuma T., Matsukura S., Kangawa K., Nakazato M. (2000) Ghrelin, a novel growth hormone-releasing acylated peptide, is synthesized in a distinct endocrine cell type in the gastrointestinal tracts of rats and humans. Endocrinology, 141:4255-4261.
    9. Du C., Xilingaowa, Cao, G., Wang, C., Li, H., Zhao, Y., Siqingaowa, Cao, J. (2009) Expression of the orexigenic peptide ghrelin in the sheep ovary. Domestic Animal Endocrinology, 36 :89–98.
    10. Duggal P.S., Weitsman S.R., Magofûn D.A. and Norman R.J. (2002) Expression of the long (OB-RB) and short (OB-RA) forms of the leptin receptor throughout the oestrous cycle in the mature rat ovary. Reproduction, 123: 899–905.
    11. Duxbury, M.S., Waseem, T., Ito, H., Robinson, M.K., Zinner, M.J., Ashley, S.W. (2003) Ghrelin promotes pancreatic adenocarcinoma cellular proliferation and invasiveness. Biochem Biophys Res Commun., 309:468.
    12. Evans H.E., Sack W.O. (1973) Prenatal development of domestic and laboratory mammals: growth curves, external features and selected references. Zentralbl Veterinarmed., 2(1):11-45.
    13. FAO, 2008, http://www.fao.org.
    14. Garcia, M.C., Lopez M., Alvarez C.V., Casanueva F., Tena-Sempere M., Dieguez C. (2007) Role of ghrelin in reproduction. Reproduction, 133:531–540.
    15. Gaytan, F., Barreiro, M. L., Chopin, L. K. (2003). “Immunolocalization of ghrelin and its functional receptor, the type 1a growth hormone secretagogue receptor, in the cyclic human ovary. J Clin Endocrinol Metab., 88: 879–887.
    16. Gualillo, O., et al., (2003). FEBS Lett., 552:105–109.
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