OPTIMIZATION OF POLYMERASE CHAIN REACTION FOR DETECTION OF INFECTIOUS BURSAL DISEASE VIRUS

Article Id: ARCC3493 | Page : 132 - 134
Citation :- OPTIMIZATION OF POLYMERASE CHAIN REACTION FOR DETECTION OF INFECTIOUS BURSAL DISEASE VIRUS.Indian Journal Of Animal Research.2001.(35):132 - 134
M.Parthiban*, T.p.Sujatha, V.Thiagarajan and R.Velmurugan
Address : Tamil Nadu Veterinary and Animal Sciences University, Chennai - 600 051, India

Abstract

Optimisation of reagents and conditions used in PCR has been carried out for detection of infectious bursal disease virus. Varying concentration of Taq DNA polymerase and dNTP mix does not much affect the PCR assay whereas varying concentration of MgCl2, IBD specific primers and annealing temperature has pronounced effect on the amplification reaction.

Keywords

References

  1. Chomczynski, P. and Sacchi, N. (1987). Anal. Biochem., 162 : 156 - 159.
  2. Hirai, K. et al. (1972). Avian Dis., 36: 221 - 226.
  3. Jackwood,P.J. and Nielson, C.K. (1997). Avian Dis., 41: 137 - 143.
  4. Lin,Z. et.al. (1993). Avain Dis., 37: 315 - 323.
  5. Liu, H.J. et al. (1994). J. Viral. Meth., 48 : 281 - 291.
  6. Qian, B. and Kibenge, E.S.B. (1994). J. Viral. Meth., 47: 237 - 242.
  7. Saiki, RT. (1989). In: PCR Technology: Principles and Application for DNA Amplification. (Erlich, HA. Ed.) New York. Shockton Press. 7-16.
  8. Wu.C.C. et al. (1992). Avian Dis., 16 : 961 - 964.

Global Footprints