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Current IssueEditorial BoardOnline First ArticlesArchive

volume 35 issue 1 (january to june 2001) : 21 - 26

EFFECT OF PETROLEUM ETHER EXTRACTS OF DIFFERENT PARTS OF NEEM SEED (AZADIRACHTA INDICA) ON HAEMOTOLOGICAL AND BIOCHEMICAL PARAMETERS IN RATS

S
Shashi Gupta
M
Meena Katana·
P
P.K.Gupta
V
V.K.Vijjan
1Division of Pharmacology and Toxicology Indian Veterinary Research Institute, lzatnagar - 243 122, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Gupta Shashi, Katana· Meena, P.K.Gupta, V.K.Vijjan (2025). EFFECT OF PETROLEUM ETHER EXTRACTS OF DIFFERENT PARTS OF NEEM SEED (AZADIRACHTA INDICA) ON HAEMOTOLOGICAL AND BIOCHEMICAL PARAMETERS IN RATS. Indian Journal of Animal Research. 35(1): 21 - 26. doi: .

ABSTRACT

The effect of daily oral administration of petroleum ether extract of neem whole seed (566 mg/kg b.wt) and husk (360 mg/kg b. wt) for a period of 60 days was studied on haematological and blood biochemical parameters of rats. A significant decrease in ESR and erythrocyte count was observed; mean corpuscular haemoglobin and mean corpuscular volume were significantly increased. No significant change in haemoglobin, haematocrit, leucocyte count and mean corpuscular haemoglobin concentration was observed. There was no significant change in blood glucose level; blood urea nitrogen was significantly increased, and SGOT and SGPT were significantly decreased. Serum protein was significantly increased during first 30 days of treatment. Serum cholestrol and plasma total lipids were significantly increased. Plasma phospholipids were significantly decreased. There was significant decrease in erythrocyte acetylcholinesterase enzyme, whereas Glutathione-s transferase was significantly increased. From this study it may be concluded that some toxic components may be present in the husk which probably is responsible for some of these physiological alterations in rats.

KEYWORDS

    REFERENCES

    1. Bhargawa, A.K. et al. (1985). Indian J. Surg. 6: 66-97.
    2. Bhargawa, A.K. (1986). J. Vet. Physiol Allied Sci. 5: 45-48.
    3. Bergmeyer, H.V. (1974). Methods in Enxymatic Analysis Vol 11. Verlag Chemic Weinheim. Academic Press, . New York.
    4. Chiaman, B.S. and Henry, R.J. (1959). Am. J. Clin. Pathol. 31: 305.
    5. Ellman, G.\. etal. (1961). Biochem. Pharmaeol. 7: 88-95.
    6. Ferings, C.S. and Fandiey, TW.(1972). Clin. Chern. 18: 673-674.
    7. Frias, RN. et aI. (1975). Bioph Biochem Acta 415: 231-251.
    8. Goldberg, D.M. and Spooner, RJ.(1983). Methods of Enzymatic .. Analysis (3rd ed.) Volll\. Enzymes I: Oxidoreductases, transferases pp. 258-265.
    9. Gupta, P.K.( 1997). In: Recent Advances on Biosciences and Oceanography. (Agar1'lw.al,V.P. et al. ed.) Society of Biosclences, Muzaffarnagar, India pp. 165-168.
    10. Gupta, Shashi, et aI. ( 1998). Indian J. Environ. Toxieol. 8: 77-80.
    11. Gupta, Shashi, et aI. (1998a). Indian J.Environ. Toxieol. 8: 89-90.
    12. Habig, w.H. etal. (1974). J. BioI. Chern. 247: 7130-7139
    13. , Haltrnan, E. (1959). Nature 183: 108-109.
    14. Hamahan, D.J., and Ekhlom, J.E. (1974). In: Enzymology, Biomembranes. (Colowik, ParetA. and Kaplan, N.O.ed.) Academic Press, New York pp 168-181.
    15. Jain, N.C.(1986). In: Schalm's Veterinary. Haemotoiogy (4th edition) pp 20-50, Lea & Febiger, Philadelphia.
    16. Jain, S.K.(1986). Biochem Biophys. Acta 937: 205-210.
    17. Kataria, Meena, et aI. (1998). Indian J. Toxieol. 5: 31-39.
    18. Lal, R, et aI. (1986). Indian J. Med. Res. 83: 89-92.
    19. Lowry,O.H.et aJ. (1951). J. BioI. Chern. 193: 265-275.
    20. Merck, E.(1970). Clinical Laboratory, Germany pp49. .
    21. Okpanyi, S.N. and Ezenkwu, G.C. (1981). Planta Med. 71: 34.
    22. Pillai, L.R and Santhakumari, G. (1981): Indian J. Pharma. 13: 91-92.
    23. 26 INDIAN JOURNAL OF ANIMAL RESEARCH
    24. Reibnan, S. and Frankel, S. (1957). Am. J. cUn. Pathol. 28: 56-63. .
    25. Schwartz, M.(1971). In: Methods of Enzymology XVII (Part B), (Calowick, S.P. and Kaplan, N.O. 00.) Academic
    26. Press, New York, pp 857-861.
    27. Shukla,R. et ai. (1973). MOOidne Surg. 11: 12.
    28. Snecdecor, W.G. and Cochran, G.W.(1976). Statistical Methods. Oxford and IBH publishing Co. Calcutta.
    29. Sorg, D.A. and Buckner, B. (1964). Proc. Exp. BioI. Moo. 115: 1131-1132.
    30. Woo, J. and Cannon, J.C.(1991). In: Clinical Diagnosis and Management by Laboratory Methods (Henry, Bernard 00.), W.B. Saunders Co.
    31. Zilvermit, D.B. and Davis, A.K. (1950). J. Lab. Clin. Moo. 36: 265.
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    Indian Journal of Animal Research
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      volume 35 issue 1 (january to june 2001) : 21 - 26

      EFFECT OF PETROLEUM ETHER EXTRACTS OF DIFFERENT PARTS OF NEEM SEED (AZADIRACHTA INDICA) ON HAEMOTOLOGICAL AND BIOCHEMICAL PARAMETERS IN RATS

      S
      Shashi Gupta
      M
      Meena Katana·
      P
      P.K.Gupta
      V
      V.K.Vijjan
      1Division of Pharmacology and Toxicology Indian Veterinary Research Institute, lzatnagar - 243 122, India

      • Submitted|

      • First Online |

      • doi

      Cite article:- Gupta Shashi, Katana· Meena, P.K.Gupta, V.K.Vijjan (2025). EFFECT OF PETROLEUM ETHER EXTRACTS OF DIFFERENT PARTS OF NEEM SEED (AZADIRACHTA INDICA) ON HAEMOTOLOGICAL AND BIOCHEMICAL PARAMETERS IN RATS. Indian Journal of Animal Research. 35(1): 21 - 26. doi: .

      ABSTRACT

      The effect of daily oral administration of petroleum ether extract of neem whole seed (566 mg/kg b.wt) and husk (360 mg/kg b. wt) for a period of 60 days was studied on haematological and blood biochemical parameters of rats. A significant decrease in ESR and erythrocyte count was observed; mean corpuscular haemoglobin and mean corpuscular volume were significantly increased. No significant change in haemoglobin, haematocrit, leucocyte count and mean corpuscular haemoglobin concentration was observed. There was no significant change in blood glucose level; blood urea nitrogen was significantly increased, and SGOT and SGPT were significantly decreased. Serum protein was significantly increased during first 30 days of treatment. Serum cholestrol and plasma total lipids were significantly increased. Plasma phospholipids were significantly decreased. There was significant decrease in erythrocyte acetylcholinesterase enzyme, whereas Glutathione-s transferase was significantly increased. From this study it may be concluded that some toxic components may be present in the husk which probably is responsible for some of these physiological alterations in rats.

      KEYWORDS

        REFERENCES

        1. Bhargawa, A.K. et al. (1985). Indian J. Surg. 6: 66-97.
        2. Bhargawa, A.K. (1986). J. Vet. Physiol Allied Sci. 5: 45-48.
        3. Bergmeyer, H.V. (1974). Methods in Enxymatic Analysis Vol 11. Verlag Chemic Weinheim. Academic Press, . New York.
        4. Chiaman, B.S. and Henry, R.J. (1959). Am. J. Clin. Pathol. 31: 305.
        5. Ellman, G.\. etal. (1961). Biochem. Pharmaeol. 7: 88-95.
        6. Ferings, C.S. and Fandiey, TW.(1972). Clin. Chern. 18: 673-674.
        7. Frias, RN. et aI. (1975). Bioph Biochem Acta 415: 231-251.
        8. Goldberg, D.M. and Spooner, RJ.(1983). Methods of Enzymatic .. Analysis (3rd ed.) Volll\. Enzymes I: Oxidoreductases, transferases pp. 258-265.
        9. Gupta, P.K.( 1997). In: Recent Advances on Biosciences and Oceanography. (Agar1'lw.al,V.P. et al. ed.) Society of Biosclences, Muzaffarnagar, India pp. 165-168.
        10. Gupta, Shashi, et aI. ( 1998). Indian J. Environ. Toxieol. 8: 77-80.
        11. Gupta, Shashi, et aI. (1998a). Indian J.Environ. Toxieol. 8: 89-90.
        12. Habig, w.H. etal. (1974). J. BioI. Chern. 247: 7130-7139
        13. , Haltrnan, E. (1959). Nature 183: 108-109.
        14. Hamahan, D.J., and Ekhlom, J.E. (1974). In: Enzymology, Biomembranes. (Colowik, ParetA. and Kaplan, N.O.ed.) Academic Press, New York pp 168-181.
        15. Jain, N.C.(1986). In: Schalm's Veterinary. Haemotoiogy (4th edition) pp 20-50, Lea & Febiger, Philadelphia.
        16. Jain, S.K.(1986). Biochem Biophys. Acta 937: 205-210.
        17. Kataria, Meena, et aI. (1998). Indian J. Toxieol. 5: 31-39.
        18. Lal, R, et aI. (1986). Indian J. Med. Res. 83: 89-92.
        19. Lowry,O.H.et aJ. (1951). J. BioI. Chern. 193: 265-275.
        20. Merck, E.(1970). Clinical Laboratory, Germany pp49. .
        21. Okpanyi, S.N. and Ezenkwu, G.C. (1981). Planta Med. 71: 34.
        22. Pillai, L.R and Santhakumari, G. (1981): Indian J. Pharma. 13: 91-92.
        23. 26 INDIAN JOURNAL OF ANIMAL RESEARCH
        24. Reibnan, S. and Frankel, S. (1957). Am. J. cUn. Pathol. 28: 56-63. .
        25. Schwartz, M.(1971). In: Methods of Enzymology XVII (Part B), (Calowick, S.P. and Kaplan, N.O. 00.) Academic
        26. Press, New York, pp 857-861.
        27. Shukla,R. et ai. (1973). MOOidne Surg. 11: 12.
        28. Snecdecor, W.G. and Cochran, G.W.(1976). Statistical Methods. Oxford and IBH publishing Co. Calcutta.
        29. Sorg, D.A. and Buckner, B. (1964). Proc. Exp. BioI. Moo. 115: 1131-1132.
        30. Woo, J. and Cannon, J.C.(1991). In: Clinical Diagnosis and Management by Laboratory Methods (Henry, Bernard 00.), W.B. Saunders Co.
        31. Zilvermit, D.B. and Davis, A.K. (1950). J. Lab. Clin. Moo. 36: 265.
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