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volume 39 issue 2 (july to december 2005) : III - 114

USEFULNESS OF NESTED POLYMERASE CHAIN REACTION IN DETECTION OF MONODON BACULOVIRUS IN SHRIMP SEEDS

A
A. Uma
M
M. Parthiban
N
N. Daniel Joy Chandran
B
B. Thiyagarajan
P
P. Kalaimani Arasi
A
A. Koteeswaran
1Vaccine Research Centre-Viral Vaccines, Centre for Animal Health Studies. Madhavaram Milk Colony. Chennai - 600 051. India

  • Submitted|

  • First Online |

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Cite article:- Uma A., Parthiban M., Chandran Joy Daniel N., Thiyagarajan B., Arasi Kalaimani P., Koteeswaran A. (2025). USEFULNESS OF NESTED POLYMERASE CHAIN REACTION IN DETECTION OF MONODON BACULOVIRUS IN SHRIMP SEEDS. Indian Journal of Animal Research. 39(2): III - 114. doi: .

ABSTRACT

Monodon baculovirus (MBV) affects the cultured shrimp and results in production and economic
losses in the shrimp hatcheries and farms. Microscopic observation of the malachite green stained
wet mount squashes from the hepatopancreas for the presence of MBV occlusion bodies is the
widely followed method to diagnose MBV. This method was compared with a sensitive nested PCR
assay to grade the level of MBV infection in shrimp seeds (postlarvae). Out of 100 shrimp seed
samples screened for MBV, 20% of the seed samples were found to be positive for MBV by both
microscopic observation and I step PCR whereas 30% of the shrimp seeds samples were found to
be positive for MBV by II step or nested PCR only, The op'imum concentration of DNA to be used
for nested PCR assay for diagnosis of MBV was also standardized in this study.

KEYWORDS

    REFERENCES

    1. Chang, P.S. et aI. (1993). J. Invert. PathoI., 62: 116-120,
    2. Flegel, T.w. et aI, (2001). Effects of hepatopancreatic parvovirus (HPV). Monodon baculovirus (MBV) and multiple viral infections on cultivated shrimp in Thailand symposium on sustainable shrimp aquaculture. Tokyo university of fisheries.
    3. Hsu, YL. et aI. (2000), Dis. Aquat. Org,. 40: 93-99.
    4. Li et aI. (2001). J. Fisheries China, 25: 141-146.
    5. Lightner, DV (1996). A Hand Book of Shrimp Pathology and Diagnostic Procedures for Diseases of Cultural Penaeid Shrimp. World Aquaculture Society, Baton Rouge. LA. USA.
    6. Poulos. B.J. et aI. (1994). J. Viral. Meth.. 49: 187-194.
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    Indian Journal of Animal Research
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      volume 39 issue 2 (july to december 2005) : III - 114

      USEFULNESS OF NESTED POLYMERASE CHAIN REACTION IN DETECTION OF MONODON BACULOVIRUS IN SHRIMP SEEDS

      A
      A. Uma
      M
      M. Parthiban
      N
      N. Daniel Joy Chandran
      B
      B. Thiyagarajan
      P
      P. Kalaimani Arasi
      A
      A. Koteeswaran
      1Vaccine Research Centre-Viral Vaccines, Centre for Animal Health Studies. Madhavaram Milk Colony. Chennai - 600 051. India

      • Submitted|

      • First Online |

      • doi

      Cite article:- Uma A., Parthiban M., Chandran Joy Daniel N., Thiyagarajan B., Arasi Kalaimani P., Koteeswaran A. (2025). USEFULNESS OF NESTED POLYMERASE CHAIN REACTION IN DETECTION OF MONODON BACULOVIRUS IN SHRIMP SEEDS. Indian Journal of Animal Research. 39(2): III - 114. doi: .

      ABSTRACT

      Monodon baculovirus (MBV) affects the cultured shrimp and results in production and economic
      losses in the shrimp hatcheries and farms. Microscopic observation of the malachite green stained
      wet mount squashes from the hepatopancreas for the presence of MBV occlusion bodies is the
      widely followed method to diagnose MBV. This method was compared with a sensitive nested PCR
      assay to grade the level of MBV infection in shrimp seeds (postlarvae). Out of 100 shrimp seed
      samples screened for MBV, 20% of the seed samples were found to be positive for MBV by both
      microscopic observation and I step PCR whereas 30% of the shrimp seeds samples were found to
      be positive for MBV by II step or nested PCR only, The op'imum concentration of DNA to be used
      for nested PCR assay for diagnosis of MBV was also standardized in this study.

      KEYWORDS

        REFERENCES

        1. Chang, P.S. et aI. (1993). J. Invert. PathoI., 62: 116-120,
        2. Flegel, T.w. et aI, (2001). Effects of hepatopancreatic parvovirus (HPV). Monodon baculovirus (MBV) and multiple viral infections on cultivated shrimp in Thailand symposium on sustainable shrimp aquaculture. Tokyo university of fisheries.
        3. Hsu, YL. et aI. (2000), Dis. Aquat. Org,. 40: 93-99.
        4. Li et aI. (2001). J. Fisheries China, 25: 141-146.
        5. Lightner, DV (1996). A Hand Book of Shrimp Pathology and Diagnostic Procedures for Diseases of Cultural Penaeid Shrimp. World Aquaculture Society, Baton Rouge. LA. USA.
        6. Poulos. B.J. et aI. (1994). J. Viral. Meth.. 49: 187-194.
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