- Venlateha. M.D. and Ramadass, P. (1999). Indian J. Anim. Sci., 69: 942-944,
- Wagenaar. J et aJ. (2001). Am. J. Vet. Res.. 61: 316-320. 65
Gene fragment of Leptospira serovar Canicola was cloned in E. coli cells using pUC18
plasmid as a vector. The recombinant cells were selected on LB agar medium, which contained
ampicillin, X-gal and IPTG. From the recombinant white colonies, plasmid was extracted and
the leptospiral DNA insert was collected after digestion with EcoR I and used as probe in
hybridization experiments.