Chief EditorM. R. Saseendranath
Print ISSN 0367-6722
Online ISSN 0976-0555
NAAS Rating 6.40
SJR 0.233, CiteScore: 0.606
Impact Factor 0.5 (2025)

Frequency :

Monthly (January, February, March, April, May, June, July, August, September, October, November and December)

Indexing Services :

Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus

Submit

Indian Journal of Animal Research, volume 39 issue 1 (january to june 2005) : 63 '. 65

CLONING OF LEPTOSPIRA CAN/COLA SEROVAR GENE IN E. COU AND USING AS A PROBE IN SLOT BLOT HYBRIDIZATION

Satyajit Saha, Nandini, M.D. Venkatesha, R.N. ~inivasa Gowda

Affiliations

¹ICAR Scheme on DNA based diagnostics for Leptospirosis. Institute of Animal Health and Veterinary Biologicals, Hebbal, Bangalore . 560 024, India

Submitted|
First Online |
doi

Cite article:- Saha Satyajit, Nandini, Venkatesha M.D., Gowda ~inivasa R.N. (2025). CLONING OF LEPTOSPIRA CAN/COLA SEROVAR GENE IN E. COU AND USING AS A PROBE IN SLOT BLOT HYBRIDIZATION. Indian Journal of Animal Research. 39(1): 63 '. 65. doi: .

ABSTRACT

Gene fragment of Leptospira serovar Canicola was cloned in E. coli cells using pUC18
plasmid as a vector. The recombinant cells were selected on LB agar medium, which contained
ampicillin, X-gal and IPTG. From the recombinant white colonies, plasmid was extracted and
the leptospiral DNA insert was collected after digestion with EcoR I and used as probe in
hybridization experiments.

KEYWORDS

REFERENCES

Venlateha. M.D. and Ramadass, P. (1999). Indian J. Anim. Sci., 69: 942-944,
Wagenaar. J et aJ. (2001). Am. J. Vet. Res.. 61: 316-320. 65

Disclaimer :

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Copyright :

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reviewed By

Share options

Editorial Board

View all (0)

CLONING OF LEPTOSPIRA CAN/COLA SEROVAR GENE IN E. COU AND USING AS A PROBE IN SLOT BLOT HYBRIDIZATION

ABSTRACT

KEYWORDS

REFERENCES

Reviewed By

In this Article

APC

Publish With US

Become a Reviewer/Member

Open Access

Products and Services

Support and Policies

Editorial Board