Article Id: ARCC3283 | Page : 167 - 169
Citation :- DETOXIFICATION OF ZEARALENONE BY CHEMISORBANTS AND YEAST IN POULTRY FEED.Indian Journal Of Animal Research.2006.(40):167 - 169
Surekha Kale1, T. Thirumalesh2 and G.V.N. Reddy
Address : College of Veterinary Science, Acharya N.G. Ranga Agricultural University, Rajendranagar, Hyderabad - 500 030, India


Lyophilized culture of Fusarium sporotrichioides was cultured on oatmeal agar. Subsequently
the fungus was grown on broken wheat for three weeks for production of zearalenone toxin on
large scale. Quantification of the cultured toxin was done using High Performance Thin Layer
Chromatagraphy and found to be 573. 12 ppm. This culture was added to poultry feed to
obtain the required level of toxin (2 ppm). This zearalenone contaminated feed was then treated
with different adsorbents : yeast culture, activated charcoal, hydrated sodium calcium alumino
silicate (HSCAS) and polyvinyl poly pyrrolidone (PVPP) at different levels to study the levels of
adsorption of the toxin. The quantity of unbound zearalenone was estimated by ELISA method.
Yeast culture and activated charcoal at 0.2 % levels adsorbed toxin to a maximum extent (81.45
and 79.45 %, respectively) and proved better than HSCAS and PVPP (40.23 and 66.90%,



  1. AOAC (1995). Official Methods of Analysis.16 edn. Association of Official Analytical Chemists, Washington DC.
  2. Bilgrami, K.S. et al. (1995). Indian Phytopath., 38: 347.
  3. Cast (1989). Mycotoxins: Economic and health risk. Task force Ref. No. 116, November, 1989. Council of Agriculture Science and Technology, Ameas, IA.
  4. Chi, M.S. (1980). Poult. Vet. Sci., 59: 531.
  5. Christensen, C.M. et al. (1965). Appl. Microbiol., 13: 653.
  6. Chung, T.K. et al. (1990). Poult. Vet. Sci., 69: 1364.
  7. Danicke, S. and Gareis, M. (2001). Kraftfutter, 1: 30-32.
  8. Eppley, R.M. (1968). J. AOAC, 51: 74.
  9. HUFF, N.E. et al. (1992). Poult. Vet. Sci., 71: 64-69.
  10. Miller, J.K. et al. (1973). Vet. Rec., 93: 555.
  11. Mirocha, C.J. (1982). Toxicol. Appl. Pharm., 66: 77.

Global Footprints