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volume 40 issue 1 (march 2006) : 91 - 92

HISTOENZYMIC REACTIVITY OF LACTIC DEHYDROGENASE IN SPERMATOZOA DURING CRYOPRESERVATION OF CROSSBRED GOAT (BEETAL x ASSAM LOCAL) SEMEN*

C
C.K. Baruah1
R
R.K. Biswas2
B
B.C. Deka
B
B.N. Borgohain
M
M. Bhattacharya3
1Department of Gynaecology, Obstetrics and A.I., College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati - 781022, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Baruah1 C.K., Biswas2 R.K., Deka B.C., Borgohain B.N., Bhattacharya3 M. (2025). HISTOENZYMIC REACTIVITY OF LACTIC DEHYDROGENASE IN SPERMATOZOA DURING CRYOPRESERVATION OF CROSSBRED GOAT (BEETAL x ASSAM LOCAL) SEMEN*. Indian Journal of Animal Research. 40(1): 91 - 92. doi: .

ABSTRACT

A total of 36 ejaculates collected from four adult crossbred (Beetal x Assam Local) goats
were used to record the Lactic Dehydrogenase(LDH) reactivity in mid piece of spermatozoa
during different stages of cryopreservation. Seminal ejaculates were divided equally and extended
in Tris-egg yolk-citric acid-glycerol extender containing either fructose, sucrose or lactose and
frozen in liquid nitrogen using French medium straw after allowing glycerol equilibration period
of either 11/2, 1 or 1/2 hour before freezing. The study revealed that the LDH reactivity of
spermatozoa was comparable when frozen after different glycerol equilibration periods suggesting
that out of the three, any convenient glycerol equilibration period could be adopted without
affecting the quality of frozen semen. The reactivity of LDH in spematozoa was higher when
frozen using Tris containing fructose which might be indicative of better cryopreservation provided
by the extender.

KEYWORDS

    REFERENCES

    1. Berndtson, W.E. and Foote, R.H.(1972). In: 7th Int. Congr. Anim Reprod. and Artif. Insem., Munich, Summaries, pp. 266 (Cf. Anim. Breed. Abstr., 41: 1067).
    2. Choudhury, A.H. et al. (1986). In: 6th National Congr. Anim Reprod., Nov. 28-30, Guwahati-22, pp. 29.
    3. Deka, B.C. (1984). Ph.D. Thesis, Andhra Pradesh Agricultural University, Hyderabad.
    4. Pearse, A.G.E (1980). Histochemistry: Theoretical and Applied. Vol. 2. Churchill Livingstone, Edinburg, pp. 1343.
    5. Puranik, S.V. et al. (1992). Indian Vet. J., 69: 74-75.
    6. Stamatiadis, K. et al. (1984). In: The Male in Farm Animal Reproduction, Martinus, Nijhoff, Boston, pp. 257-263. (Cf. Anim. Breed. Abstr., 53: 2203).
    7. Wiggin, H.B. and Almquist, J.O. (1975). J. Dairy Sci., 58: 416-419.
    8. Wootton, I.D.P. (1964). Microanalysis in Medical Biochemistry. 4th edn. Churchill Ltd., Gloucester Place, London, pp. 104.
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    Indian Journal of Animal Research
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      volume 40 issue 1 (march 2006) : 91 - 92

      HISTOENZYMIC REACTIVITY OF LACTIC DEHYDROGENASE IN SPERMATOZOA DURING CRYOPRESERVATION OF CROSSBRED GOAT (BEETAL x ASSAM LOCAL) SEMEN*

      C
      C.K. Baruah1
      R
      R.K. Biswas2
      B
      B.C. Deka
      B
      B.N. Borgohain
      M
      M. Bhattacharya3
      1Department of Gynaecology, Obstetrics and A.I., College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati - 781022, India

      • Submitted|

      • First Online |

      • doi

      Cite article:- Baruah1 C.K., Biswas2 R.K., Deka B.C., Borgohain B.N., Bhattacharya3 M. (2025). HISTOENZYMIC REACTIVITY OF LACTIC DEHYDROGENASE IN SPERMATOZOA DURING CRYOPRESERVATION OF CROSSBRED GOAT (BEETAL x ASSAM LOCAL) SEMEN*. Indian Journal of Animal Research. 40(1): 91 - 92. doi: .

      ABSTRACT

      A total of 36 ejaculates collected from four adult crossbred (Beetal x Assam Local) goats
      were used to record the Lactic Dehydrogenase(LDH) reactivity in mid piece of spermatozoa
      during different stages of cryopreservation. Seminal ejaculates were divided equally and extended
      in Tris-egg yolk-citric acid-glycerol extender containing either fructose, sucrose or lactose and
      frozen in liquid nitrogen using French medium straw after allowing glycerol equilibration period
      of either 11/2, 1 or 1/2 hour before freezing. The study revealed that the LDH reactivity of
      spermatozoa was comparable when frozen after different glycerol equilibration periods suggesting
      that out of the three, any convenient glycerol equilibration period could be adopted without
      affecting the quality of frozen semen. The reactivity of LDH in spematozoa was higher when
      frozen using Tris containing fructose which might be indicative of better cryopreservation provided
      by the extender.

      KEYWORDS

        REFERENCES

        1. Berndtson, W.E. and Foote, R.H.(1972). In: 7th Int. Congr. Anim Reprod. and Artif. Insem., Munich, Summaries, pp. 266 (Cf. Anim. Breed. Abstr., 41: 1067).
        2. Choudhury, A.H. et al. (1986). In: 6th National Congr. Anim Reprod., Nov. 28-30, Guwahati-22, pp. 29.
        3. Deka, B.C. (1984). Ph.D. Thesis, Andhra Pradesh Agricultural University, Hyderabad.
        4. Pearse, A.G.E (1980). Histochemistry: Theoretical and Applied. Vol. 2. Churchill Livingstone, Edinburg, pp. 1343.
        5. Puranik, S.V. et al. (1992). Indian Vet. J., 69: 74-75.
        6. Stamatiadis, K. et al. (1984). In: The Male in Farm Animal Reproduction, Martinus, Nijhoff, Boston, pp. 257-263. (Cf. Anim. Breed. Abstr., 53: 2203).
        7. Wiggin, H.B. and Almquist, J.O. (1975). J. Dairy Sci., 58: 416-419.
        8. Wootton, I.D.P. (1964). Microanalysis in Medical Biochemistry. 4th edn. Churchill Ltd., Gloucester Place, London, pp. 104.
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