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Current IssueEditorial BoardOnline First ArticlesArchive

volume 41 issue 3 (september 2007) : 226 - 229

PCR BASED DETECTION OF ADHESIVE CURLI GENE “crl” AND ‘csgA’ IN AVIAN PATHOGENIC ESCHERICHIA COLI*

M
Manju Pal1
S
S.D. Singh2
1Division of Avian Diseases, Indian Veterinary Research Institute, Izatnagar (U.P.) - 243 122, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Pal1 Manju, Singh2 S.D. (2025). PCR BASED DETECTION OF ADHESIVE CURLI GENE “crl” AND ‘csgA’ IN AVIAN PATHOGENIC ESCHERICHIA COLI*. Indian Journal of Animal Research. 41(3): 226 - 229. doi: .

ABSTRACT

Twelve Escherichia coli isolates from colibacillosis cases of chickens were investigated for
the presence of genes encoding ‘curli adhesin (crl and csgA) by polymerase chain reaction using
two different sets of primers. The results showed the presence of crl gene in 7 isolates and csgA
gene in 5 isolates out of 12 isolates tested. Curli genes were ubiquitous among E.coli. Further,
Almost all the 12 isolates having curli genes were found to have haemagglutination (HA) ability
suggesting possible role of curli adhesin to mediate heamagglutination and enhancing pathogenecity process.

KEYWORDS

    REFERENCES

    1. Collinson, S.K. et al. (1991). J. Bacteriol., 173: 4773-4781.
    2. Delicato, E.R. et al. (2003). Vet. Microbiol., 94: 97-103.
    3. Dho-Moulin, M. and Fairbrother, J.M. (1999). Vet. Res., 30: 299-316.
    4. Dozois, C.M. et al. (1992). Infection and Immunity, 60(7): 2648-2656.
    5. Gross, W.G. (1994). In: Diseases Due to Escherichia in Poultry. (Gyles, C.L. ed). CAB International, Wallingford,
    6. pp. 237-259.
    7. Hulgren, S.J. et al. (1993). Cell, 73: 887-901.
    8. Hulgren, S.J. et al. (1996). In: Escherichia coli and Salmonella typhimurium : Cellular and Molecular Biology.
    9. (Neithardt, F.C. ed.), American Society for Microbiology, Washington D.C., pp. 2730-2756.
    10. 228 INDIAN JOURNAL OF ANIMAL RESEARCH
    11. Knobl, T. et al. (2001). Vet. Microbio., 83: 71-80.
    12. Maurer, J.J. et al. (1998). Avian Diseases, 42: 106-118.
    13. Olsen, A. et al. (1989). Nature, 338-655.
    14. Pourbakhsh, S.A. et al. (1997). Vet. Microbiol., 58: 195-213.
    15. Provence, D.L. and Curtiss, R. (1992). Infection and Immunity, 60: 4460-4467.
    16. Oyofo, B.A. et al. (1989). Poultry Sci., 68: 1351-1356.
    17. Wooley, R.E. et al. (1992). Avian Dis., 36: 679-684.
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    Indian Journal of Animal Research
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      volume 41 issue 3 (september 2007) : 226 - 229

      PCR BASED DETECTION OF ADHESIVE CURLI GENE “crl” AND ‘csgA’ IN AVIAN PATHOGENIC ESCHERICHIA COLI*

      M
      Manju Pal1
      S
      S.D. Singh2
      1Division of Avian Diseases, Indian Veterinary Research Institute, Izatnagar (U.P.) - 243 122, India

      • Submitted|

      • First Online |

      • doi

      Cite article:- Pal1 Manju, Singh2 S.D. (2025). PCR BASED DETECTION OF ADHESIVE CURLI GENE “crl” AND ‘csgA’ IN AVIAN PATHOGENIC ESCHERICHIA COLI*. Indian Journal of Animal Research. 41(3): 226 - 229. doi: .

      ABSTRACT

      Twelve Escherichia coli isolates from colibacillosis cases of chickens were investigated for
      the presence of genes encoding ‘curli adhesin (crl and csgA) by polymerase chain reaction using
      two different sets of primers. The results showed the presence of crl gene in 7 isolates and csgA
      gene in 5 isolates out of 12 isolates tested. Curli genes were ubiquitous among E.coli. Further,
      Almost all the 12 isolates having curli genes were found to have haemagglutination (HA) ability
      suggesting possible role of curli adhesin to mediate heamagglutination and enhancing pathogenecity process.

      KEYWORDS

        REFERENCES

        1. Collinson, S.K. et al. (1991). J. Bacteriol., 173: 4773-4781.
        2. Delicato, E.R. et al. (2003). Vet. Microbiol., 94: 97-103.
        3. Dho-Moulin, M. and Fairbrother, J.M. (1999). Vet. Res., 30: 299-316.
        4. Dozois, C.M. et al. (1992). Infection and Immunity, 60(7): 2648-2656.
        5. Gross, W.G. (1994). In: Diseases Due to Escherichia in Poultry. (Gyles, C.L. ed). CAB International, Wallingford,
        6. pp. 237-259.
        7. Hulgren, S.J. et al. (1993). Cell, 73: 887-901.
        8. Hulgren, S.J. et al. (1996). In: Escherichia coli and Salmonella typhimurium : Cellular and Molecular Biology.
        9. (Neithardt, F.C. ed.), American Society for Microbiology, Washington D.C., pp. 2730-2756.
        10. 228 INDIAN JOURNAL OF ANIMAL RESEARCH
        11. Knobl, T. et al. (2001). Vet. Microbio., 83: 71-80.
        12. Maurer, J.J. et al. (1998). Avian Diseases, 42: 106-118.
        13. Olsen, A. et al. (1989). Nature, 338-655.
        14. Pourbakhsh, S.A. et al. (1997). Vet. Microbiol., 58: 195-213.
        15. Provence, D.L. and Curtiss, R. (1992). Infection and Immunity, 60: 4460-4467.
        16. Oyofo, B.A. et al. (1989). Poultry Sci., 68: 1351-1356.
        17. Wooley, R.E. et al. (1992). Avian Dis., 36: 679-684.
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