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Current IssueEditorial BoardOnline First ArticlesArchive

volume 41 issue 2 (june 2007) : 106 - 110

ENZYMATIC PROFILES OF ACID AND ALKALINE PHOSPHATASES IN OVARIAN ANTRAL FOLLICULAR FLUID OF BUFFALOES*

G
G.P. Kalmath
J
J.P. Ravindra1**
1Department of Veterinary Physiology, Veterinary College, Bangalore - 560 024, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Kalmath G.P., Ravindra1** J.P. (2025). ENZYMATIC PROFILES OF ACID AND ALKALINE PHOSPHATASES IN OVARIAN ANTRAL FOLLICULAR FLUID OF BUFFALOES*. Indian Journal of Animal Research. 41(2): 106 - 110. doi: .

ABSTRACT

Enzymatic profiles of alkaline and acid phosphatases in the buffalo ovarian antral follicular
fluid were studied using ovaries procured from Civil Slaughterhouse, Bangalore. Antral follicular
fluid aspirated from small (6 mm), medium (6-10 mm) andlarge (11-16 mm) follicles was
analyzed for phosphatase activity using clinical analyzer (photometer, BT-224 Biotechnica
instruments). The acid phosphatase activity decreased with advancement in follicular size reaching
the lowest value in large follicle group. Similar trend was observed with respect to the alkaline
phosphatase activity. The acid and alkaline phosphatase activities were highest during the
hotter months of April and June when the monthly average mean temperatures were (27.200C)
and (25.850C) respectively. It was lowest during the cooler months of December and January,
when the ambient temperatures were (21.200C) and (20.950C) respectively. However during
July and August, the activity was intermediate. From the study it was concluded that the
enzyme activity decreased with the follicular development and it increased with the increase in
ambient temperature

KEYWORDS

    REFERENCES

    1. Atheya, U.K. and Totey, S.M. (2002). Buffalo J., 18(1): 137-141.
    2. Caucing, H. et al. Gynecol. Invest., 3: 215.
    3. Chang, S.C.S. et al. (1976). Biol. Reprod., 15: 321-28.
    4. Chauhan, M.S. et al. (1997). Theriogenology, 48: 461-69.
    5. Faulker, W.R. and Meitis, S. (1982). Selected Methods for the Small Clinical Chemistry Laboratory. Washington,
    6. D.C., pp. 125.
    7. Henderson, K.A. and Cupps, P.T. (1990). J. Anim. Sci., 68: 1363-69.
    8. Vol. 41, No. 2, 2007 109
    9. Henry, J.B. (1984). Clinical Diagnosis and Management. 17th Ed. W.B. Saunders Co., Philadelphia, pp. 157.
    10. Ireland, J.J. and Roche, J.F. (1983). Endocrinology, 112: 150-56.
    11. Kulkarni, B.A. (1988). In II world buffalo congress, Vol. III: 233-38.
    12. Lobel, B.L. and Levy, E. (1968). Endocrinol. Suppl., 132: 7.
    13. Madan, M.C. et al. (1998). J. Anim. Reprod., 19(2): 160.
    14. McGaughey, R.W. (1975). Biol. Reprod., 13: 147.
    15. Mekkawy, M.Y. et al. (1988). Alexandria J. Vet. Sci., 4: 391-96.
    16. Palta, P. et al. (1998). Indian J. Anim. Sci., 68(5): 444-447.
    17. Pandey, M.D. and Razada, B.C. (1979). In: Proceedings of the FAO seminar on Buffalo Reproduction and AI
    18. (Karnal), pp. 235-245.
    19. Raghava, R.V. et al. (1997). Buffalo J., 13: 237-42.
    20. Singh, N. and Rajya, B.S. (1982). Indian J. Anim. Hlth., 6: 1-3.
    21. Sirard, M.A. et al. (1995). Theriogenology, 44(1): 85-94.
    22. Snedecor, G.W. and Cochran, W.G. (1994). Statistical Methods. 8th Ed., Affiliated East West Press, New Delhi.
    23. Tietz, N.W. (1976). Fundamentals of Clinical Chemistry, Philadelphia, W.B. Saunders Co., pp. 614-874.
    24. Van Kempen, H. (1978). M.S. Thesis. University of California, Davis.
    25. Wise, T. (1987). J. Anim. Sci., 64: 1153-1169.
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    Indian Journal of Animal Research
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      volume 41 issue 2 (june 2007) : 106 - 110

      ENZYMATIC PROFILES OF ACID AND ALKALINE PHOSPHATASES IN OVARIAN ANTRAL FOLLICULAR FLUID OF BUFFALOES*

      G
      G.P. Kalmath
      J
      J.P. Ravindra1**
      1Department of Veterinary Physiology, Veterinary College, Bangalore - 560 024, India

      • Submitted|

      • First Online |

      • doi

      Cite article:- Kalmath G.P., Ravindra1** J.P. (2025). ENZYMATIC PROFILES OF ACID AND ALKALINE PHOSPHATASES IN OVARIAN ANTRAL FOLLICULAR FLUID OF BUFFALOES*. Indian Journal of Animal Research. 41(2): 106 - 110. doi: .

      ABSTRACT

      Enzymatic profiles of alkaline and acid phosphatases in the buffalo ovarian antral follicular
      fluid were studied using ovaries procured from Civil Slaughterhouse, Bangalore. Antral follicular
      fluid aspirated from small (6 mm), medium (6-10 mm) andlarge (11-16 mm) follicles was
      analyzed for phosphatase activity using clinical analyzer (photometer, BT-224 Biotechnica
      instruments). The acid phosphatase activity decreased with advancement in follicular size reaching
      the lowest value in large follicle group. Similar trend was observed with respect to the alkaline
      phosphatase activity. The acid and alkaline phosphatase activities were highest during the
      hotter months of April and June when the monthly average mean temperatures were (27.200C)
      and (25.850C) respectively. It was lowest during the cooler months of December and January,
      when the ambient temperatures were (21.200C) and (20.950C) respectively. However during
      July and August, the activity was intermediate. From the study it was concluded that the
      enzyme activity decreased with the follicular development and it increased with the increase in
      ambient temperature

      KEYWORDS

        REFERENCES

        1. Atheya, U.K. and Totey, S.M. (2002). Buffalo J., 18(1): 137-141.
        2. Caucing, H. et al. Gynecol. Invest., 3: 215.
        3. Chang, S.C.S. et al. (1976). Biol. Reprod., 15: 321-28.
        4. Chauhan, M.S. et al. (1997). Theriogenology, 48: 461-69.
        5. Faulker, W.R. and Meitis, S. (1982). Selected Methods for the Small Clinical Chemistry Laboratory. Washington,
        6. D.C., pp. 125.
        7. Henderson, K.A. and Cupps, P.T. (1990). J. Anim. Sci., 68: 1363-69.
        8. Vol. 41, No. 2, 2007 109
        9. Henry, J.B. (1984). Clinical Diagnosis and Management. 17th Ed. W.B. Saunders Co., Philadelphia, pp. 157.
        10. Ireland, J.J. and Roche, J.F. (1983). Endocrinology, 112: 150-56.
        11. Kulkarni, B.A. (1988). In II world buffalo congress, Vol. III: 233-38.
        12. Lobel, B.L. and Levy, E. (1968). Endocrinol. Suppl., 132: 7.
        13. Madan, M.C. et al. (1998). J. Anim. Reprod., 19(2): 160.
        14. McGaughey, R.W. (1975). Biol. Reprod., 13: 147.
        15. Mekkawy, M.Y. et al. (1988). Alexandria J. Vet. Sci., 4: 391-96.
        16. Palta, P. et al. (1998). Indian J. Anim. Sci., 68(5): 444-447.
        17. Pandey, M.D. and Razada, B.C. (1979). In: Proceedings of the FAO seminar on Buffalo Reproduction and AI
        18. (Karnal), pp. 235-245.
        19. Raghava, R.V. et al. (1997). Buffalo J., 13: 237-42.
        20. Singh, N. and Rajya, B.S. (1982). Indian J. Anim. Hlth., 6: 1-3.
        21. Sirard, M.A. et al. (1995). Theriogenology, 44(1): 85-94.
        22. Snedecor, G.W. and Cochran, W.G. (1994). Statistical Methods. 8th Ed., Affiliated East West Press, New Delhi.
        23. Tietz, N.W. (1976). Fundamentals of Clinical Chemistry, Philadelphia, W.B. Saunders Co., pp. 614-874.
        24. Van Kempen, H. (1978). M.S. Thesis. University of California, Davis.
        25. Wise, T. (1987). J. Anim. Sci., 64: 1153-1169.
        In this Article
          Contact us
          Follow us
          Published In
          Indian Journal of Animal Research

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