Forty female (Swiss albino) mice were divided into 4 groups of ten animals each. Mice of
groups I, II and III were fed sucralose 500, 1000 and 1500 ppm, respectively in feed. Group IV
was fed sucralose free diet. Five animals were sacrificed from each group on 30th and 60th day
of study and metaphase spreads prepared for evaluation of mitotic index and chromosomal
aberrations. Liver and kidney samples of mice were also collected on 30th and 60th day of
experiment for histopathological study. Among the chromosomal aberrations observed, polyploidy
and monosomy were most common. The differences in mitotic indices and chromosomal
aberrations between the 4 groups at different intervals were found to be statistically nonsignificant.
Histopathological changes were observed in the liver and kidney of all treated groups.
It is concluded from the present study that sucralose consumption up to 1500 ppm for two
months did not produce any genotoxicity but it is hepatotoxic and nephrotoxic. However, its
effect at higher doses and longer duration needs to be studies.