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Indian Journal of Animal Research
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volume 42 issue 4 (december 2008) : 285-287
SPERM NUCLEAR CHROMATIN DECONDENSATION TEST : ITS APPLICABILITY TO PREDICT FERTILITY OF CRYOPRESERVED SEMEN SAMPLES
1Animal Genetics and Breeding Division,
National Dairy Res. Institutes, Karnal-132 001, India
ABSTRACT
The nuclear chromatin decondensation test (NCDT) detects the ability of the sperm chromatin
to undergo decondensation following fertilization. The test does not have direct bearing with the
fertilizing ability of the spermatozoa but only reflects the proportion of sperm that may swell following
exposure to in vitro decondensing agents. The gross seminal parameters do not have any
direct relationship on the ability of the sperm chromatin to effect decondensation whether in vivo or
in vitro. The test can not be reliably used to assess the fertilizing potential of frozen thawed
semen of bovines.
to undergo decondensation following fertilization. The test does not have direct bearing with the
fertilizing ability of the spermatozoa but only reflects the proportion of sperm that may swell following
exposure to in vitro decondensing agents. The gross seminal parameters do not have any
direct relationship on the ability of the sperm chromatin to effect decondensation whether in vivo or
in vitro. The test can not be reliably used to assess the fertilizing potential of frozen thawed
semen of bovines.
KEYWORDS
REFERENCES
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- Bedford, J.M. et al. (1973). J. Reprod. Fertil. 33:19-29.
- Coelingh, J.P. et al. (1972). Biochem. Biophys. ACTA, 285:1-14.
- Gopalkrishnan, K. et al. (1991). Arch. Androl., 27:43-58.
- Jadhav, A. (1998). Ph. D. Thesis. National Dairy Research Institute, Karnal, Haryana, India.
- Snedecor, G.W. and Cochran, W.G. (1989). Statistical Methods. 8th Ed.: lowa University Press, Ames, lowa.
- Wagner, T.E. et al. (1978). Arch. Androl., 1:31-41.
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Published In
Indian Journal of Animal Research