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Current IssueEditorial BoardOnline First ArticlesArchive

volume 42 issue 4 (december 2008) : 279-281

PROTOCOL FOR ISOLATION OF GENOMIC DNA FROM LIVESTOCK BLOOD FOR MICROSATELLITE ANALYSIS*

D
D.B. Shinde**
B
B.V. Gujar
H
H.B. Patil
S
S.A. Satpute
U
U.B. Kashid
1College of Agricultural Biotechnology, Latur (M.S.), India

  • Submitted|

  • First Online |

  • doi

Cite article:- Shinde** D.B., Gujar B.V., Patil H.B., Satpute S.A., Kashid U.B. (2025). PROTOCOL FOR ISOLATION OF GENOMIC DNA FROM LIVESTOCK BLOOD FOR MICROSATELLITE ANALYSIS*. Indian Journal of Animal Research. 42(4): 279-281. doi: .

ABSTRACT

Isolation of pure high molecular weight genomic DNA is a pre-requisite in microsatellite studies.
The phenol-chloroform method of DNA extraction from whole blood usually involves several steps
which are time consuming. Further the phenol is corrosive and toxic. A simple procedure called
High salt method was further optimized for isolation of high molecular weight genomic DNA from
much lesser quantity of buffalo blood i.e. only from 200 μl, which helped in avoiding wastage of
blood sample and also saving costly chemicals. The optimized High salt method produced good
yield of pure high molecular weight genomic DNA.

KEYWORDS

    REFERENCES

    1. Andersson, L. et al. (1986). Anim. Genet., 17:95-112.
    2. Blin, N. and Stafford, D.W. (1976). Nucleic Acids Res., 3:2303-2308.
    3. Jeffreys, A.J. et al. (1985). Nature, 314:67-73.
    4. Litt, M. and Luty, J.A. (1989). Am. J. Hum. Genet., 44:397-401.
    5. Martin, Burriel, I. et al. (1999). Anim. Genet., 30:177-182.
    6. Moazami-Goudarzi, K. et al. (1997). Anim. Genet., 30:338-345.
    7. Montgomery, G.W. and Sise, I.A. (1990). NZ J. Agric. Res., 33:437-441.
    8. Ritz, L.R. et al. (2000). Anim. Genet., 31(3):178-185.
    9. Senthil, M. et al. (1996). Int. J. Anim. Sci., 11:413-414.
    10. Takezaki, N. and Nei, M. (1996). Genet., 114:389-399.
    11. Viaman, M. et al. (1986). Anim. Genet., 17:113-133.
    12. Yadav, B.R. et al. (2003). Manual for Training in Genome Analysis Using Molecular Markers in Farm Animals. NDRI, Karnal.
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    Indian Journal of Animal Research
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      volume 42 issue 4 (december 2008) : 279-281

      PROTOCOL FOR ISOLATION OF GENOMIC DNA FROM LIVESTOCK BLOOD FOR MICROSATELLITE ANALYSIS*

      D
      D.B. Shinde**
      B
      B.V. Gujar
      H
      H.B. Patil
      S
      S.A. Satpute
      U
      U.B. Kashid
      1College of Agricultural Biotechnology, Latur (M.S.), India

      • Submitted|

      • First Online |

      • doi

      Cite article:- Shinde** D.B., Gujar B.V., Patil H.B., Satpute S.A., Kashid U.B. (2025). PROTOCOL FOR ISOLATION OF GENOMIC DNA FROM LIVESTOCK BLOOD FOR MICROSATELLITE ANALYSIS*. Indian Journal of Animal Research. 42(4): 279-281. doi: .

      ABSTRACT

      Isolation of pure high molecular weight genomic DNA is a pre-requisite in microsatellite studies.
      The phenol-chloroform method of DNA extraction from whole blood usually involves several steps
      which are time consuming. Further the phenol is corrosive and toxic. A simple procedure called
      High salt method was further optimized for isolation of high molecular weight genomic DNA from
      much lesser quantity of buffalo blood i.e. only from 200 μl, which helped in avoiding wastage of
      blood sample and also saving costly chemicals. The optimized High salt method produced good
      yield of pure high molecular weight genomic DNA.

      KEYWORDS

        REFERENCES

        1. Andersson, L. et al. (1986). Anim. Genet., 17:95-112.
        2. Blin, N. and Stafford, D.W. (1976). Nucleic Acids Res., 3:2303-2308.
        3. Jeffreys, A.J. et al. (1985). Nature, 314:67-73.
        4. Litt, M. and Luty, J.A. (1989). Am. J. Hum. Genet., 44:397-401.
        5. Martin, Burriel, I. et al. (1999). Anim. Genet., 30:177-182.
        6. Moazami-Goudarzi, K. et al. (1997). Anim. Genet., 30:338-345.
        7. Montgomery, G.W. and Sise, I.A. (1990). NZ J. Agric. Res., 33:437-441.
        8. Ritz, L.R. et al. (2000). Anim. Genet., 31(3):178-185.
        9. Senthil, M. et al. (1996). Int. J. Anim. Sci., 11:413-414.
        10. Takezaki, N. and Nei, M. (1996). Genet., 114:389-399.
        11. Viaman, M. et al. (1986). Anim. Genet., 17:113-133.
        12. Yadav, B.R. et al. (2003). Manual for Training in Genome Analysis Using Molecular Markers in Farm Animals. NDRI, Karnal.
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