PROTOCOL FOR ISOLATION OF GENOMIC DNA FROM LIVESTOCK BLOOD FOR MICROSATELLITE ANALYSIS*

Article Id: ARCC2393 | Page : 279-281
Citation :- PROTOCOL FOR ISOLATION OF GENOMIC DNA FROM LIVESTOCK BLOOD FOR MICROSATELLITE ANALYSIS*.Indian Journal Of Animal Research.2008.(42):279-281
D.B. Shinde**, B.V. Gujar, H.B. Patil, S.A. Satpute and U.B. Kashid
Address : College of Agricultural Biotechnology, Latur (M.S.), India

Abstract

Isolation of pure high molecular weight genomic DNA is a pre-requisite in microsatellite studies.
The phenol-chloroform method of DNA extraction from whole blood usually involves several steps
which are time consuming. Further the phenol is corrosive and toxic. A simple procedure called
High salt method was further optimized for isolation of high molecular weight genomic DNA from
much lesser quantity of buffalo blood i.e. only from 200 μl, which helped in avoiding wastage of
blood sample and also saving costly chemicals. The optimized High salt method produced good
yield of pure high molecular weight genomic DNA.

Keywords

References

  1. Andersson, L. et al. (1986). Anim. Genet., 17:95-112.
  2. Blin, N. and Stafford, D.W. (1976). Nucleic Acids Res., 3:2303-2308.
  3. Jeffreys, A.J. et al. (1985). Nature, 314:67-73.
  4. Litt, M. and Luty, J.A. (1989). Am. J. Hum. Genet., 44:397-401.
  5. Martin, Burriel, I. et al. (1999). Anim. Genet., 30:177-182.
  6. Moazami-Goudarzi, K. et al. (1997). Anim. Genet., 30:338-345.
  7. Montgomery, G.W. and Sise, I.A. (1990). NZ J. Agric. Res., 33:437-441.
  8. Ritz, L.R. et al. (2000). Anim. Genet., 31(3):178-185.
  9. Senthil, M. et al. (1996). Int. J. Anim. Sci., 11:413-414.
  10. Takezaki, N. and Nei, M. (1996). Genet., 114:389-399.
  11. Viaman, M. et al. (1986). Anim. Genet., 17:113-133.
  12. Yadav, B.R. et al. (2003). Manual for Training in Genome Analysis Using Molecular Markers in Farm Animals. NDRI, Karnal.

Global Footprints