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Current IssueEditorial BoardOnline First ArticlesArchive

volume 42 issue 3 (september 2008) : 222-224

DETECTION OF BRUCELLA DNA IN SEMEN USING THE POLYMERASE CHAIN REACTION ASSAY

A
A.N. Kanani
L
Lata Jain
T
T.J. Patel
D
D.N. Rank
C
C.G. Joshi
J
J.H. Purohit
1Department of Veterinary Microbiology, College of Veterinary Science and animal husbandry, Anand Agricultural University, Anand-388 001, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Kanani A.N., Jain Lata, Patel T.J., Rank D.N., Joshi C.G., Purohit J.H. (2025). DETECTION OF BRUCELLA DNA IN SEMEN USING THE POLYMERASE CHAIN REACTION ASSAY. Indian Journal of Animal Research. 42(3): 222-224. doi: .

ABSTRACT

Polymerase chain reaction (PCR) assay was employed for detection of Brucella DNA in semen
of cattle and buffalo breeding bulls. A genus specific primer pair which amplified a 223 bp fragment
of gene encoding a 31-kDa Brucella abortus antigen was used in assay. Serological test for the
diagnosis of Brucella infection was also performed in breeding bulls. The PCR was resulted in more
numbers of positive samples as compared to serological test since it detected Brucella DNA in 19
out of 101semen samples while Brucella antibodies was detected in 10 sera samples of same
bulls using ELISA. The PCR may be used as supplementary test for detection of Brucella
infection in bulls.

KEYWORDS

    REFERENCES

    1. Amin, A. S. et al. (2001). Vet. Microbiol., 83: 37-44.
    2. Anonymous, (2000). Annual Report 1999-2000. Biotechnology Laboratory. National Dairy Development
    3. Board, Anand.
    4. Baily, G.C. et al. (1992). J. Trop. Med. Hyg., 95: 271-275.
    5. Cetinkaya, B. et al. (1999). Vet. Rec., 144: 239-240.
    6. Chatterjee, A. et al. (1986). Indian Vet. J., 63: 95-100.
    7. Cortez, A. et al. (2001). Aust. Vet. J., 79: 500-501.
    8. Fekete, A. et al. (1992). J. Vet. Diagn. Invest., 4: 79-83.
    9. Gallien, P. et al. (1998). Vet. Rec., 142: 512-514.
    10. Guarino, A. et al. (2000). Vet. Rec., 147: 634-636.
    11. Gupta, V. K. et al. (2006). Indian J. Anim. Sci., 76: 793-795.
    12. Leal-Klevezas, D. S. et al. (2000). Vet. Microbiol., 75: 91-97.
    13. Leal-Klevezas, D. S. et al. (1995). J. Clin. Microbiol., 3: 3087-3090.
    14. Manterola, L. et al. (2003). Vet. Microbiol., 92: 65-72.
    15. Manthei, C. A. et al. (1950). In: Proc. Am. Vet. Med. Assoc., 87: 177-184.
    16. Mittal, V. et al. (2005). Indian J. Vet. Med., 25: 28-32.
    17. Morgan, W. J. B. and MacKinnon, D. J. (1979). In: Fertility and Infertility in Domestic Animals. (Laing, J. A.,ed), 3rd
    18. Edition. ELBS, Bailliere Tindall. pp. 171-198.
    19. Romero, C. et al. (1995). J. Clin. Microbiol., 33: 3198-3200.
    20. Sarumathi, C. et al. (2003). Indian J. Dairy Sci., 56: 408-410
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      volume 42 issue 3 (september 2008) : 222-224

      DETECTION OF BRUCELLA DNA IN SEMEN USING THE POLYMERASE CHAIN REACTION ASSAY

      A
      A.N. Kanani
      L
      Lata Jain
      T
      T.J. Patel
      D
      D.N. Rank
      C
      C.G. Joshi
      J
      J.H. Purohit
      1Department of Veterinary Microbiology, College of Veterinary Science and animal husbandry, Anand Agricultural University, Anand-388 001, India

      • Submitted|

      • First Online |

      • doi

      Cite article:- Kanani A.N., Jain Lata, Patel T.J., Rank D.N., Joshi C.G., Purohit J.H. (2025). DETECTION OF BRUCELLA DNA IN SEMEN USING THE POLYMERASE CHAIN REACTION ASSAY. Indian Journal of Animal Research. 42(3): 222-224. doi: .

      ABSTRACT

      Polymerase chain reaction (PCR) assay was employed for detection of Brucella DNA in semen
      of cattle and buffalo breeding bulls. A genus specific primer pair which amplified a 223 bp fragment
      of gene encoding a 31-kDa Brucella abortus antigen was used in assay. Serological test for the
      diagnosis of Brucella infection was also performed in breeding bulls. The PCR was resulted in more
      numbers of positive samples as compared to serological test since it detected Brucella DNA in 19
      out of 101semen samples while Brucella antibodies was detected in 10 sera samples of same
      bulls using ELISA. The PCR may be used as supplementary test for detection of Brucella
      infection in bulls.

      KEYWORDS

        REFERENCES

        1. Amin, A. S. et al. (2001). Vet. Microbiol., 83: 37-44.
        2. Anonymous, (2000). Annual Report 1999-2000. Biotechnology Laboratory. National Dairy Development
        3. Board, Anand.
        4. Baily, G.C. et al. (1992). J. Trop. Med. Hyg., 95: 271-275.
        5. Cetinkaya, B. et al. (1999). Vet. Rec., 144: 239-240.
        6. Chatterjee, A. et al. (1986). Indian Vet. J., 63: 95-100.
        7. Cortez, A. et al. (2001). Aust. Vet. J., 79: 500-501.
        8. Fekete, A. et al. (1992). J. Vet. Diagn. Invest., 4: 79-83.
        9. Gallien, P. et al. (1998). Vet. Rec., 142: 512-514.
        10. Guarino, A. et al. (2000). Vet. Rec., 147: 634-636.
        11. Gupta, V. K. et al. (2006). Indian J. Anim. Sci., 76: 793-795.
        12. Leal-Klevezas, D. S. et al. (2000). Vet. Microbiol., 75: 91-97.
        13. Leal-Klevezas, D. S. et al. (1995). J. Clin. Microbiol., 3: 3087-3090.
        14. Manterola, L. et al. (2003). Vet. Microbiol., 92: 65-72.
        15. Manthei, C. A. et al. (1950). In: Proc. Am. Vet. Med. Assoc., 87: 177-184.
        16. Mittal, V. et al. (2005). Indian J. Vet. Med., 25: 28-32.
        17. Morgan, W. J. B. and MacKinnon, D. J. (1979). In: Fertility and Infertility in Domestic Animals. (Laing, J. A.,ed), 3rd
        18. Edition. ELBS, Bailliere Tindall. pp. 171-198.
        19. Romero, C. et al. (1995). J. Clin. Microbiol., 33: 3198-3200.
        20. Sarumathi, C. et al. (2003). Indian J. Dairy Sci., 56: 408-410
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