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volume 42 issue 3 (september 2008) : 201-204

IN VITRO ACROSOME REACTION : ITS PERTINENCE IN ASSESSING FERTILITY OF CRYOPRESERVED SEMEN

C
C.S. Mukhopadhyay
A
Archana Verma
B
B.K. Joshi
A
Avtar Singh
A
A.K. Chakravarty
P
P. P. Dubey
1Animal Genetics and Breeding Division, National Dairy Research Institute, Karnal-132001, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Mukhopadhyay C.S., Verma Archana, Joshi B.K., Singh Avtar, Chakravarty A.K., Dubey P. P. (2025). IN VITRO ACROSOME REACTION : ITS PERTINENCE IN ASSESSING FERTILITY OF CRYOPRESERVED SEMEN. Indian Journal of Animal Research. 42(3): 201-204. doi: .

ABSTRACT

Cryopreserved spermatozoa are prone to cryoinjury which induces membrane damage and
render them incapable to undergo acrosome reaction (AR). In the present study, cryopreserved
semen samples obtained from 30 Karan Fries bulls were subjected to in vitro AR. The AR scores
were correlated with the fertility rates (conception rates) following A.I. with frozen semen frozen
semen. The AR scores and gross seminal parameters showed significant correlations except for
semen volume. A prediction equation (R2 = 37.3) was constructed from AR scores and was used to
predict the conception rates of donor bulls. The predicted and observed conception rates did not
show any significant difference for chi square test. The study indicates that in vitro acrosome
reaction test may be complemented with the conventional semen analyses tests prior to using
cryopreserved semen samples for AI purpose.

KEYWORDS

    REFERENCES

    1. Alveraz, J. G. and Storey, B. T. (1993) J. Androl. 14: 199-208.
    2. Anonymous (1996). SYSTAT 6.0.1 for Windows: Statistics SPSS Inc Michigan Avenue Chicago IL USA.
    3. Benjamin, B. R. et al. (1987). Indian J. Anim. Sci. 57: 1175-1180.
    4. Berger, T. et al., (1989). Biol. Reprod. 40: 525 – 530.
    5. Brucker, C. and Lipford, G. B. (1995). Hum. Reprod. Update. 1: 51-62.
    6. Critszer, J.K. et al., (1987). Fertil. Steril. 47: 980-84.
    7. Hoing, L. M. et al., (1986). Fertil. Steril. 45: 499-505.
    8. Jadhav, A. 1998. Ph. D. Thesis. National Dairy Research Institute, Karnal, Haryana, India.
    9. Jeyendran, R. et al., (1984). J. Reprod. Fertil. 70: 219- 228.
    10. Mc Laughlin, K. J. et al., (1990). Reprod. Fertil. Develop. 2: 619-622.
    11. Meizel, S. et al., (1980). Dev. Growth Differen. 22: 483-494.
    12. Neild, D. N. et al., (2005). Anim.Reprod. Sci. 89: 47-56.
    13. 204 INDIAN JOURNAL OF ANIMAL RESEARCH
    14. Payne, D. et al., (1994). Hum. Reprod. 9: 1281-1288.
    15. Snedecor, G. W. and Cochran W. G. (1989). Statistical Methods. 8th ed. Iowa University Press Ames Iowa.
    16. Ucar, O. (2004). Kafkas Üniversitesi Veteriner Fakultesi Dergisi. 10: 117-124.
    17. Vazquez , J. M. et al., (2005). Mol. Reprod. Dev. 36: 84 – 88.
    18. Whitfield, C. H. and Parkinson, T. J. (1995). Therio. 44: 413 – 422.
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      volume 42 issue 3 (september 2008) : 201-204

      IN VITRO ACROSOME REACTION : ITS PERTINENCE IN ASSESSING FERTILITY OF CRYOPRESERVED SEMEN

      C
      C.S. Mukhopadhyay
      A
      Archana Verma
      B
      B.K. Joshi
      A
      Avtar Singh
      A
      A.K. Chakravarty
      P
      P. P. Dubey
      1Animal Genetics and Breeding Division, National Dairy Research Institute, Karnal-132001, India

      • Submitted|

      • First Online |

      • doi

      Cite article:- Mukhopadhyay C.S., Verma Archana, Joshi B.K., Singh Avtar, Chakravarty A.K., Dubey P. P. (2025). IN VITRO ACROSOME REACTION : ITS PERTINENCE IN ASSESSING FERTILITY OF CRYOPRESERVED SEMEN. Indian Journal of Animal Research. 42(3): 201-204. doi: .

      ABSTRACT

      Cryopreserved spermatozoa are prone to cryoinjury which induces membrane damage and
      render them incapable to undergo acrosome reaction (AR). In the present study, cryopreserved
      semen samples obtained from 30 Karan Fries bulls were subjected to in vitro AR. The AR scores
      were correlated with the fertility rates (conception rates) following A.I. with frozen semen frozen
      semen. The AR scores and gross seminal parameters showed significant correlations except for
      semen volume. A prediction equation (R2 = 37.3) was constructed from AR scores and was used to
      predict the conception rates of donor bulls. The predicted and observed conception rates did not
      show any significant difference for chi square test. The study indicates that in vitro acrosome
      reaction test may be complemented with the conventional semen analyses tests prior to using
      cryopreserved semen samples for AI purpose.

      KEYWORDS

        REFERENCES

        1. Alveraz, J. G. and Storey, B. T. (1993) J. Androl. 14: 199-208.
        2. Anonymous (1996). SYSTAT 6.0.1 for Windows: Statistics SPSS Inc Michigan Avenue Chicago IL USA.
        3. Benjamin, B. R. et al. (1987). Indian J. Anim. Sci. 57: 1175-1180.
        4. Berger, T. et al., (1989). Biol. Reprod. 40: 525 – 530.
        5. Brucker, C. and Lipford, G. B. (1995). Hum. Reprod. Update. 1: 51-62.
        6. Critszer, J.K. et al., (1987). Fertil. Steril. 47: 980-84.
        7. Hoing, L. M. et al., (1986). Fertil. Steril. 45: 499-505.
        8. Jadhav, A. 1998. Ph. D. Thesis. National Dairy Research Institute, Karnal, Haryana, India.
        9. Jeyendran, R. et al., (1984). J. Reprod. Fertil. 70: 219- 228.
        10. Mc Laughlin, K. J. et al., (1990). Reprod. Fertil. Develop. 2: 619-622.
        11. Meizel, S. et al., (1980). Dev. Growth Differen. 22: 483-494.
        12. Neild, D. N. et al., (2005). Anim.Reprod. Sci. 89: 47-56.
        13. 204 INDIAN JOURNAL OF ANIMAL RESEARCH
        14. Payne, D. et al., (1994). Hum. Reprod. 9: 1281-1288.
        15. Snedecor, G. W. and Cochran W. G. (1989). Statistical Methods. 8th ed. Iowa University Press Ames Iowa.
        16. Ucar, O. (2004). Kafkas Üniversitesi Veteriner Fakultesi Dergisi. 10: 117-124.
        17. Vazquez , J. M. et al., (2005). Mol. Reprod. Dev. 36: 84 – 88.
        18. Whitfield, C. H. and Parkinson, T. J. (1995). Therio. 44: 413 – 422.
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