volume 42 issue 2 (june 2008) : 122-124
DEVELOPMENT OF IN-VITRO PRODUCED CAPRINE EMBRYOS IN VARIOUS CULTURE SYSTEMS
1Department of Animal Production Physiology,
CCS Haryana Agricultural University, Hisar - 125004, India
Published In
Indian Journal of Animal Research
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volume 42 issue 2 (june 2008) : 122-124
DEVELOPMENT OF IN-VITRO PRODUCED CAPRINE EMBRYOS IN VARIOUS CULTURE SYSTEMS
1Department of Animal Production Physiology,
CCS Haryana Agricultural University, Hisar - 125004, India
ABSTRACT
The efficacy of various culture systems was studied for the development of in-vitro produced
Caprine embryos . For this purpose, 2 to 4 cell stage caprine embryos matured and fertilized under
in vitro conditions were cultured with four culture systems (cumulus cell monolayers, oviductal cell
monolayers, chick embryo and rabbit peritoneal fluid) and a control system (TCM-199 + FCS) for
about 5 days. The maximum development rate of 48.67±4.08 per cent was obtained when rPF
(rabbit peritoneal fluid) was used as the culture system, while none of the 2-4 cell stage embryos
reached the morulae/blastocyst stage in the control group. The development rates obtained in other
groups were 18.67±3.18, 28.00±3.67 and 45.00±6.42 per cent, when embryos were cultured with
cumulus cell monolayers, oviductal cell monolayers and in the fertile chicken eggs amniotic cavity,
respectively. It was, thus, concluded that rPF was found to be the most effective culture system for
the development of caprine embryos.
Caprine embryos . For this purpose, 2 to 4 cell stage caprine embryos matured and fertilized under
in vitro conditions were cultured with four culture systems (cumulus cell monolayers, oviductal cell
monolayers, chick embryo and rabbit peritoneal fluid) and a control system (TCM-199 + FCS) for
about 5 days. The maximum development rate of 48.67±4.08 per cent was obtained when rPF
(rabbit peritoneal fluid) was used as the culture system, while none of the 2-4 cell stage embryos
reached the morulae/blastocyst stage in the control group. The development rates obtained in other
groups were 18.67±3.18, 28.00±3.67 and 45.00±6.42 per cent, when embryos were cultured with
cumulus cell monolayers, oviductal cell monolayers and in the fertile chicken eggs amniotic cavity,
respectively. It was, thus, concluded that rPF was found to be the most effective culture system for
the development of caprine embryos.
KEYWORDS
REFERENCES
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- Collas, P. et al. (1991) Biol. Reprod; 44: 100-107
- Didion, B.A. et al. (1989). Gamete Res. 22: 51-57.
- Malik, R. K. et al. (1999). Anim. Reprod. Sci. 54 : 195-201..
- Parris, J. J. et al. (1986). Therrogendogy 25 : 591-600.
- Younis, A.I. and Brackett, B.G. (1991). Theriogenology 36 : 11-21.
- Zhang, L. et al. (1993). Vet. Rec. 35: 247-249.
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In this Article
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Published In
Indian Journal of Animal Research