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Current IssueEditorial BoardOnline First ArticlesArchive

volume 47 issue 5 (october 2013) : 431-434

ACUTE ORAL TOXICITY OF RAW ARECA NUT EXTRACT IN RATS

T
T.S. Lohith*
N
N.B. Shridhar
K
K. Jayakumar
M
M.L. Sathyanarayana
S
S.M. Dilip
K
K. Hemanna Gowda
1Department of Animal Husbandry & Veterinary Services Gundumgere, Doddaballapura Talluk, Bangalore– 561 203, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Lohith* T.S., Shridhar N.B., Jayakumar K., Sathyanarayana M.L., Dilip S.M., Gowda Hemanna K. (2025). ACUTE ORAL TOXICITY OF RAW ARECA NUT EXTRACT IN RATS. Indian Journal of Animal Research. 47(5): 431-434. doi: .

ABSTRACT

The aqueous extract of raw areca nut extract was evaluated for its acute oral toxicity study in rats. Healthy male and female Wistar albino rats aged between 10 to 12 weeks and weighing 190 to 210 g adult were selected. The animals were fasted overnight and raw areca nut extract in graded doses was administered as a single dose by gavage using a gavaging tube. Careful general clinical observations were made for 14 days. The toxicity signs were depression, profuse salivation, tremors, muscular incoordination, convulsions, writhing movement of neck and severe diarrhoea. The LD50 values of raw areca nut extract in male and female rats were found to be 2321.96 and 2257.52 mg/kg, respectively. The LD50 value in female rats was slightly lower than that of the male rats.

REFERENCES

  1. AOAC. (1995). Official Methods of Analysis. Association of Official Analytical Chemists. 16th Ed. Washington, DC.
  2. Clarke, M.L., Harvery, D.G. and Humphreys, D.J. (1981). Veterinary toxicology. In: Introduction Edt. Hodge, H.C. and Sterner, J.H. 2nd Ed. Bailliere Tindall. p. 8.
  3. Farnsworth, E.R. (1976). Betel nut - Its composition, chemistry and uses. Sciences in New Guinea. 4: 85–90.
  4. Finny, D.J. (1971). Statistical Methods in Biological Assay. 2nd Ed. Griffen London.
  5. Gilani, A.H., Ghayur, M.N., Zafar, S.S., Shahida, P.A., Choudhary, M.I. and Assad, K. (2004). Presence of cholinomimetic and acetylcholinesterase inhibitory constituents in betel nut. Life Sci., 75: 2377-2389.
  6. Luna, L.G. (1968). Manual of Histopathological Staining Methods of the Armed Forces Institute of Pathology. 3rd Ed. McGrow Hill Book Co., New York. p. 1-46.
  7. OECD 423. (2001). Organization for Economic Co-operation and Development (OECD) guideline for the testing of chemicals, Acute oral toxicity – Acute toxic class method.
  8. Shridhar, N. B. and Narayana, K. (2003). Toxicity study of areca extract in cross-bred calves. Paper presented in 4th conference of Society of Toxicology held at Chennai on November 21-23. p. 36.
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Indian Journal of Animal Research
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    volume 47 issue 5 (october 2013) : 431-434

    ACUTE ORAL TOXICITY OF RAW ARECA NUT EXTRACT IN RATS

    T
    T.S. Lohith*
    N
    N.B. Shridhar
    K
    K. Jayakumar
    M
    M.L. Sathyanarayana
    S
    S.M. Dilip
    K
    K. Hemanna Gowda
    1Department of Animal Husbandry & Veterinary Services Gundumgere, Doddaballapura Talluk, Bangalore– 561 203, India

    • Submitted|

    • First Online |

    • doi

    Cite article:- Lohith* T.S., Shridhar N.B., Jayakumar K., Sathyanarayana M.L., Dilip S.M., Gowda Hemanna K. (2025). ACUTE ORAL TOXICITY OF RAW ARECA NUT EXTRACT IN RATS. Indian Journal of Animal Research. 47(5): 431-434. doi: .

    ABSTRACT

    The aqueous extract of raw areca nut extract was evaluated for its acute oral toxicity study in rats. Healthy male and female Wistar albino rats aged between 10 to 12 weeks and weighing 190 to 210 g adult were selected. The animals were fasted overnight and raw areca nut extract in graded doses was administered as a single dose by gavage using a gavaging tube. Careful general clinical observations were made for 14 days. The toxicity signs were depression, profuse salivation, tremors, muscular incoordination, convulsions, writhing movement of neck and severe diarrhoea. The LD50 values of raw areca nut extract in male and female rats were found to be 2321.96 and 2257.52 mg/kg, respectively. The LD50 value in female rats was slightly lower than that of the male rats.

    REFERENCES

    1. AOAC. (1995). Official Methods of Analysis. Association of Official Analytical Chemists. 16th Ed. Washington, DC.
    2. Clarke, M.L., Harvery, D.G. and Humphreys, D.J. (1981). Veterinary toxicology. In: Introduction Edt. Hodge, H.C. and Sterner, J.H. 2nd Ed. Bailliere Tindall. p. 8.
    3. Farnsworth, E.R. (1976). Betel nut - Its composition, chemistry and uses. Sciences in New Guinea. 4: 85–90.
    4. Finny, D.J. (1971). Statistical Methods in Biological Assay. 2nd Ed. Griffen London.
    5. Gilani, A.H., Ghayur, M.N., Zafar, S.S., Shahida, P.A., Choudhary, M.I. and Assad, K. (2004). Presence of cholinomimetic and acetylcholinesterase inhibitory constituents in betel nut. Life Sci., 75: 2377-2389.
    6. Luna, L.G. (1968). Manual of Histopathological Staining Methods of the Armed Forces Institute of Pathology. 3rd Ed. McGrow Hill Book Co., New York. p. 1-46.
    7. OECD 423. (2001). Organization for Economic Co-operation and Development (OECD) guideline for the testing of chemicals, Acute oral toxicity – Acute toxic class method.
    8. Shridhar, N. B. and Narayana, K. (2003). Toxicity study of areca extract in cross-bred calves. Paper presented in 4th conference of Society of Toxicology held at Chennai on November 21-23. p. 36.
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