EXPLORING FIBROUS BYPRODUCTS AS SUBSTRATES TO GENERATE FIBROLYTIC ENZYMES

Article Id: ARCC169 | Page : 421-425
Citation :- EXPLORING FIBROUS BYPRODUCTS AS SUBSTRATES TO GENERATE FIBROLYTIC ENZYMES.Indian Journal Of Animal Research.2013.(47):421-425
S. Saravanakumar, C. Valli and V. Balakrishnan
Address : Department of Animal Nutrition, Madras Veterinary College, Chennai- 600 001, India

Abstract

This research was focused to identify suitable fibrous byproducts to grow Trichoderma viridae and optimize the conditions for the production of fibrolytic enzymes viz. cellulase and xylanase. An experiment was carried out on agro-industrial fibrous byproducts viz, coconut coir pith, saw dust, groundnut shells, paddy husk and sugarcane bagasse to select best substrates for Trichoderma viridae. The selection was based on nine measurements on a score card that gave weightage to parameters such as physical growth of Trichoderma viridae, absence of contamination, spore count on the 10th day, cellulase and xylanase activity of enzyme extract on 10th day. Among the agro-industrial fibrous byproducts paddy husk inoculated at the rate of 106 spores / 5 gram in 10 days of incubation at 30°C at pH 5 and 70 per cent moisture showed good physical growth of Trichoderma viridae, with no contamination, had higher spore count and was the only substrate that evinced activity of cellulase and xylanase.

Keywords

Fibrolytic enzymes Fibrous byproducts Paddy husk Trichoderma viridae.

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