volume 43 issue 2 (june 2009) : 136-138
EFFECT OF SEMINAL PLASMA ON QUANTIlY AND QUALIlY OF PROTEINS LEAKED DURING HEPARIN - INDUCED IN VITRO CAPACITATION OF BULL SPERM
1Department of Zoology
Punjab Agricultural University, Ludhiana- 141 004, India.
Published In
Indian Journal of Animal Research
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volume 43 issue 2 (june 2009) : 136-138
EFFECT OF SEMINAL PLASMA ON QUANTIlY AND QUALIlY OF PROTEINS LEAKED DURING HEPARIN - INDUCED IN VITRO CAPACITATION OF BULL SPERM
1Department of Zoology
Punjab Agricultural University, Ludhiana- 141 004, India.
ABSTRACT
The effect of seminal plasma on protein changes in sperm membrane during in vitro induction
of capacitation. revealed that seminal plasma caused a gradual increase in protein leakage when
added to the incubation medium (1M) in gradually higher dilutions ranging from 1:10 to 1:200.
The protein leakage was significantly higher with 1:200 (2.22±0.13 to 3.29±0.42 1ng/108s) than
with 1:10 diluted seminal plasma (0.17±0.11 to 0.62 ± 0.77 mg/108s) and control (2.0±0.27 to
2.83±0.34 mg/108s) in six bulls. The protein profile of the 1M revealed the leakage of proteins
with molecular weight of 205, 97.4, 66, 43, 29, 14.3, 6.5 and 3 kDa in all the six bulls after
addition of 1:10 as well as 1:200 diluted seminal plasma and these were similar to those found in
control. This revealed that addition of seminal plasma did not inhibit the leakage of specific proteins which further supported its stimulatory effect on capacitation in terms of protein leakage which was perhaps essential for increasing membrane fluidity leading to acrosome reaction
of capacitation. revealed that seminal plasma caused a gradual increase in protein leakage when
added to the incubation medium (1M) in gradually higher dilutions ranging from 1:10 to 1:200.
The protein leakage was significantly higher with 1:200 (2.22±0.13 to 3.29±0.42 1ng/108s) than
with 1:10 diluted seminal plasma (0.17±0.11 to 0.62 ± 0.77 mg/108s) and control (2.0±0.27 to
2.83±0.34 mg/108s) in six bulls. The protein profile of the 1M revealed the leakage of proteins
with molecular weight of 205, 97.4, 66, 43, 29, 14.3, 6.5 and 3 kDa in all the six bulls after
addition of 1:10 as well as 1:200 diluted seminal plasma and these were similar to those found in
control. This revealed that addition of seminal plasma did not inhibit the leakage of specific proteins which further supported its stimulatory effect on capacitation in terms of protein leakage which was perhaps essential for increasing membrane fluidity leading to acrosome reaction
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In this Article
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Published In
Indian Journal of Animal Research