Banner
Current IssueEditorial BoardOnline First ArticlesArchive
Current IssueEditorial BoardOnline First ArticlesArchive

volume 43 issue 2 (june 2009) : 111-113

A COMPARATIVE STUDY BETWEEN MICROSCOPIC AGGLUTINATION TEST AND DIFFERENT PROTEIN ANTIGENS BASED ENZVME-LINKED IMMUNOSORBENT' ASSAY FOR THE DETECTION OF LEPTOSPIROSIS IN BOVINE

S
Surya Sankar
H
Hiron M. Harshan
P
Pallab Chaudhary
S
S. K. Srivastava
1Division of Bacteriology and Mycology Indian Veterinary Research Institute, Izatnagar-243 122, India.

  • Submitted|

  • First Online |

  • doi

Cite article:- Sankar Surya, Harshan M. Hiron, Chaudhary Pallab, Srivastava K. S. (2025). A COMPARATIVE STUDY BETWEEN MICROSCOPIC AGGLUTINATION TEST AND DIFFERENT PROTEIN ANTIGENS BASED ENZVME-LINKED IMMUNOSORBENT' ASSAY FOR THE DETECTION OF LEPTOSPIROSIS IN BOVINE. Indian Journal of Animal Research. 43(2): 111-113. doi: .

ABSTRACT

The present study compared the efficacy of Microscopic Agglutination Test (MAT) with UpL32
and UpL41 recombinant antigen based Enzyme-linked immunosorbent assay (ELISA) for the
diagnosis of bovine leptospirosis. The UpL32 and 41 genes were amplified using specific primers
and the polymerase chain reaction products (peR) were cloned. The gene inserts from positive
recombinant clones were inserted into expression vector and transformed in Escherichia coli DH5il
cells. The recombinant UpL32 and 41 proteins were expressed, purified and standardized. The
optimum antigen concentration of 100ng of each anti..;en was used for the screening of serum
samples from cattle suspected of leptospirosis. It was found that recombinant antigen ELISA detected
more positive cases of illness than MAT. Between the two recombinant antigens ELISA, UpL32 ELISA
was able to detect more positives.

REFERENCES

  1. Biolin, C.A, et al. (1989). Am. J. Vet. Res., 50: 1001-1003.
  2. Cousins, D.V., et al. (1985). Vet. Microbiol., 10: 439-450.
  3. Engvall, E. and Perlmann, P. (1971). Immuno Chern., 8: 871-874.
  4. Faine S. (1982). Guidelines for the Control of Leptospirosis. 67. WHO Offset Publication, Geneva, 172pp.
  5. flannery, B., et a/. (2001). J. Clin. Microbiol., 39(9): 3303-3310.
  6. Maria Rosa, Q.B., et al. (2005). Vet. Microbiol., 109: 89-94.
  7. Mariya, R., etal. (2006). Compo Immunol. Microbiol. Infect. Dis., 29(5-6): 269-77.
  8. Pappas, M.G., et a/. (1985). Am. J. Trop. Med. Hyg., 34: 346-354.
  9. Thierman, AB. and Gareit, L.A (1983). Am. J .Vet. Res., 44: 883-887.
  10. Thrusfield, M. (1995). Veterinary Epidemiology, 2nded. The Blackwell Science Publications, U. K.
  11. Viajyachari, P., et al. (2001). Indian J. Med. Res., 114: 99-106.
  12. Wright, P.w., et al. (1993). Rev. Sci. Tech. Off. Int. Epiz., 12: 435-450.
Published In
Indian Journal of Animal Research
Article Metrics
Metrics Icon
0
Views
0
Citations
Reviewed By
Share Article
Download Article
In this Article
    Contact us
    Follow us

    volume 43 issue 2 (june 2009) : 111-113

    A COMPARATIVE STUDY BETWEEN MICROSCOPIC AGGLUTINATION TEST AND DIFFERENT PROTEIN ANTIGENS BASED ENZVME-LINKED IMMUNOSORBENT' ASSAY FOR THE DETECTION OF LEPTOSPIROSIS IN BOVINE

    S
    Surya Sankar
    H
    Hiron M. Harshan
    P
    Pallab Chaudhary
    S
    S. K. Srivastava
    1Division of Bacteriology and Mycology Indian Veterinary Research Institute, Izatnagar-243 122, India.

    • Submitted|

    • First Online |

    • doi

    Cite article:- Sankar Surya, Harshan M. Hiron, Chaudhary Pallab, Srivastava K. S. (2025). A COMPARATIVE STUDY BETWEEN MICROSCOPIC AGGLUTINATION TEST AND DIFFERENT PROTEIN ANTIGENS BASED ENZVME-LINKED IMMUNOSORBENT' ASSAY FOR THE DETECTION OF LEPTOSPIROSIS IN BOVINE. Indian Journal of Animal Research. 43(2): 111-113. doi: .

    ABSTRACT

    The present study compared the efficacy of Microscopic Agglutination Test (MAT) with UpL32
    and UpL41 recombinant antigen based Enzyme-linked immunosorbent assay (ELISA) for the
    diagnosis of bovine leptospirosis. The UpL32 and 41 genes were amplified using specific primers
    and the polymerase chain reaction products (peR) were cloned. The gene inserts from positive
    recombinant clones were inserted into expression vector and transformed in Escherichia coli DH5il
    cells. The recombinant UpL32 and 41 proteins were expressed, purified and standardized. The
    optimum antigen concentration of 100ng of each anti..;en was used for the screening of serum
    samples from cattle suspected of leptospirosis. It was found that recombinant antigen ELISA detected
    more positive cases of illness than MAT. Between the two recombinant antigens ELISA, UpL32 ELISA
    was able to detect more positives.

    REFERENCES

    1. Biolin, C.A, et al. (1989). Am. J. Vet. Res., 50: 1001-1003.
    2. Cousins, D.V., et al. (1985). Vet. Microbiol., 10: 439-450.
    3. Engvall, E. and Perlmann, P. (1971). Immuno Chern., 8: 871-874.
    4. Faine S. (1982). Guidelines for the Control of Leptospirosis. 67. WHO Offset Publication, Geneva, 172pp.
    5. flannery, B., et a/. (2001). J. Clin. Microbiol., 39(9): 3303-3310.
    6. Maria Rosa, Q.B., et al. (2005). Vet. Microbiol., 109: 89-94.
    7. Mariya, R., etal. (2006). Compo Immunol. Microbiol. Infect. Dis., 29(5-6): 269-77.
    8. Pappas, M.G., et a/. (1985). Am. J. Trop. Med. Hyg., 34: 346-354.
    9. Thierman, AB. and Gareit, L.A (1983). Am. J .Vet. Res., 44: 883-887.
    10. Thrusfield, M. (1995). Veterinary Epidemiology, 2nded. The Blackwell Science Publications, U. K.
    11. Viajyachari, P., et al. (2001). Indian J. Med. Res., 114: 99-106.
    12. Wright, P.w., et al. (1993). Rev. Sci. Tech. Off. Int. Epiz., 12: 435-450.
    In this Article
      Contact us
      Follow us
      Published In
      Indian Journal of Animal Research

      Editorial Board

      View all (0)