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Current IssueEditorial BoardOnline First ArticlesArchive

volume 43 issue 2 (june 2009) : 89-93

DETECTION OF MICROSATELLITE MARKERS IN POLYACRYLAMIDE GELS BY A FAST AND SENSITIVE SILVER STAINING METHOD USING BUFFALO BREEDS·

N
n.B. Shinde
B
B.V. GUiar
A
A.S. JinturKar
S
S.B. Adangale'
1Marathwada Agricultural University, Parbhani 431402, India.

  • Submitted|

  • First Online |

  • doi

Cite article:- Shinde n.B., GUiar B.V., JinturKar A.S., Adangale' S.B. (2025). DETECTION OF MICROSATELLITE MARKERS IN POLYACRYLAMIDE GELS BY A FAST AND SENSITIVE SILVER STAINING METHOD USING BUFFALO BREEDS·. Indian Journal of Animal Research. 43(2): 89-93. doi: .

ABSTRACT

A fast, reliable, cheap and sensitive silver staining method to detect microsatellite markers in
polyacrylamide gel was modified from the two standard staining procedures. The main differences
between standard method and modified. method concerned are the pre-exposure of gel with
formaldehyde during silver nitrate impregnation, the addition of sodium hydroxidf? instead of
sodium thiosulphate and sodium carbonate during development, the inclusion of the absolute
ethanol with acetic acid in the stop solution and the duration of the different reaction steps. The
modified method allowed the detection of polymorphisms for simple sequence repeats (SSR)
with different buffalo breeds with the lower backgrounds and higher conservation duration of the
gels and allowed reutilization of most of the solutions used in the staining procedure for 4-5
times, making it quite cheap

REFERENCES

  1. Barker, J.S.F. et al. (1997). Anim. Genet., 28:103-115.
  2. Barker, J.S.F. eta/. (2001). J. Anim. Breed. Genet., 118:213-233.
  3. Bassam, B.J. et a/. (1991). Anal. Biochem. 196: 80-83.
  4. Beidler,J.I et a/. (1982). Anal. Biochem. 126: 374-380.
  5. Blum, H. et al. (1987).Electrophoresis. 8:93-99.
  6. Brezinsky, L. eta/. (1993)', Anim. Genet., 24:73.
  7. Christensen,M. et a/.(1999).Scand.J.Clin.Lab.Invest. 59:167-178.
  8. Goldman,O.and Merril, C.R. (1982). Electrophoresis. 3:24-26.
  9. Halima, B. etal. (2006).Biotechnol. Agron. Soc.Environ. 10(2),77-81.
  10. U MH et a/.(2002). Genet.Sel.EvoI.34(6):729-744.
  11. Luikart, G. et a/. (1999). Aim. Genet., 30:431-438.
  12. Maudet C. et a/. (2002t. Mol. Ecol., 11:421-436.
  13. Merril, C.R. etal. (1981). Science. 211:1437-1438.
  14. Moioli, B. etal. (2001). Livestock Production Sci., 70:203-211.
  15. Ritz, L.R. et a/. (2000). Anim. Genet., 31(3):178cI85
  16. Sanguinetti, C.J. eta/. (1994). Biotechniques.17:915-919.
  17. Senthil, M. et al. (1996). Int. J. Anim. Sd, 11:413-414.
  18. Sommerville, L.L.and Wang, K. (1981). Biochem. Biophys. Res Commun. 120:530-548.
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Indian Journal of Animal Research
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    volume 43 issue 2 (june 2009) : 89-93

    DETECTION OF MICROSATELLITE MARKERS IN POLYACRYLAMIDE GELS BY A FAST AND SENSITIVE SILVER STAINING METHOD USING BUFFALO BREEDS·

    N
    n.B. Shinde
    B
    B.V. GUiar
    A
    A.S. JinturKar
    S
    S.B. Adangale'
    1Marathwada Agricultural University, Parbhani 431402, India.

    • Submitted|

    • First Online |

    • doi

    Cite article:- Shinde n.B., GUiar B.V., JinturKar A.S., Adangale' S.B. (2025). DETECTION OF MICROSATELLITE MARKERS IN POLYACRYLAMIDE GELS BY A FAST AND SENSITIVE SILVER STAINING METHOD USING BUFFALO BREEDS·. Indian Journal of Animal Research. 43(2): 89-93. doi: .

    ABSTRACT

    A fast, reliable, cheap and sensitive silver staining method to detect microsatellite markers in
    polyacrylamide gel was modified from the two standard staining procedures. The main differences
    between standard method and modified. method concerned are the pre-exposure of gel with
    formaldehyde during silver nitrate impregnation, the addition of sodium hydroxidf? instead of
    sodium thiosulphate and sodium carbonate during development, the inclusion of the absolute
    ethanol with acetic acid in the stop solution and the duration of the different reaction steps. The
    modified method allowed the detection of polymorphisms for simple sequence repeats (SSR)
    with different buffalo breeds with the lower backgrounds and higher conservation duration of the
    gels and allowed reutilization of most of the solutions used in the staining procedure for 4-5
    times, making it quite cheap

    REFERENCES

    1. Barker, J.S.F. et al. (1997). Anim. Genet., 28:103-115.
    2. Barker, J.S.F. eta/. (2001). J. Anim. Breed. Genet., 118:213-233.
    3. Bassam, B.J. et a/. (1991). Anal. Biochem. 196: 80-83.
    4. Beidler,J.I et a/. (1982). Anal. Biochem. 126: 374-380.
    5. Blum, H. et al. (1987).Electrophoresis. 8:93-99.
    6. Brezinsky, L. eta/. (1993)', Anim. Genet., 24:73.
    7. Christensen,M. et a/.(1999).Scand.J.Clin.Lab.Invest. 59:167-178.
    8. Goldman,O.and Merril, C.R. (1982). Electrophoresis. 3:24-26.
    9. Halima, B. etal. (2006).Biotechnol. Agron. Soc.Environ. 10(2),77-81.
    10. U MH et a/.(2002). Genet.Sel.EvoI.34(6):729-744.
    11. Luikart, G. et a/. (1999). Aim. Genet., 30:431-438.
    12. Maudet C. et a/. (2002t. Mol. Ecol., 11:421-436.
    13. Merril, C.R. etal. (1981). Science. 211:1437-1438.
    14. Moioli, B. etal. (2001). Livestock Production Sci., 70:203-211.
    15. Ritz, L.R. et a/. (2000). Anim. Genet., 31(3):178cI85
    16. Sanguinetti, C.J. eta/. (1994). Biotechniques.17:915-919.
    17. Senthil, M. et al. (1996). Int. J. Anim. Sd, 11:413-414.
    18. Sommerville, L.L.and Wang, K. (1981). Biochem. Biophys. Res Commun. 120:530-548.
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