Indian Journal of Animal Research

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Indian Journal of Animal Research, volume 43 issue 2 (june 2009) : 89-93

DETECTION OF MICROSATELLITE MARKERS IN POLYACRYLAMIDE GELS BY A FAST AND SENSITIVE SILVER STAINING METHOD USING BUFFALO BREEDS·

n.B. Shinde, B.V. GUiar, A.S. JinturKar, S.B. Adangale'
1Marathwada Agricultural University, Parbhani 431402, India.

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Cite article:- Shinde n.B., GUiar B.V., JinturKar A.S., Adangale' S.B. (2024). DETECTION OF MICROSATELLITE MARKERS IN POLYACRYLAMIDE GELS BY A FAST AND SENSITIVE SILVER STAINING METHOD USING BUFFALO BREEDS·. Indian Journal of Animal Research. 43(2): 89-93. doi: .

ABSTRACT

A fast, reliable, cheap and sensitive silver staining method to detect microsatellite markers in
polyacrylamide gel was modified from the two standard staining procedures. The main differences
between standard method and modified. method concerned are the pre-exposure of gel with
formaldehyde during silver nitrate impregnation, the addition of sodium hydroxidf? instead of
sodium thiosulphate and sodium carbonate during development, the inclusion of the absolute
ethanol with acetic acid in the stop solution and the duration of the different reaction steps. The
modified method allowed the detection of polymorphisms for simple sequence repeats (SSR)
with different buffalo breeds with the lower backgrounds and higher conservation duration of the
gels and allowed reutilization of most of the solutions used in the staining procedure for 4-5
times, making it quite cheap

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