Banner
Current IssueEditorial BoardOnline First ArticlesArchive
Current IssueEditorial BoardOnline First ArticlesArchive

volume 47 issue 4 (august 2013) : 335-339

DISSECTING THE PARTIAL GENOMIC REGION OF CXCR TO CORRELATE WITH CMT IN VRINDAVANI CATTLE

M
Mahesh Shivanand Dige
S
S.P.S. Ahlawat
B
Bharat Bhusan*
P
Pushpendra Kumar
A
Anuj Chauhan
B
B. Inamdar
L
L.S. Kokate
T
Triveni Dutt1
1Division of Animal Genetics and Breeding, Indian Veterinary Research Institute, Izatnagar, Bareilly-243 122, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Dige Shivanand Mahesh, Ahlawat S.P.S., Bhusan* Bharat, Kumar Pushpendra, Chauhan Anuj, Inamdar B., Kokate L.S., Dutt1 Triveni (2025). DISSECTING THE PARTIAL GENOMIC REGION OF CXCR TO CORRELATE WITH CMT IN VRINDAVANI CATTLE. Indian Journal of Animal Research. 47(4): 335-339. doi: .

ABSTRACT

Genetic markers associated with inflammatory responses during mastitis could aid in selection of cattle for mastitis resistance. The genomic region of 256 bp of CXCR2, a chemokine receptor required for neutrophil migration to infection sites was investigated in the current study to identify genetic polymorphism if any and to associate it with subclinical and clinical mastitis. Ninety five Vrindavani crossbred cows (42-mastitis tolerant and 53- with clinical mastitis) who completed at least two full lactations were taken for study. The cows which had never been affected by clinical mastitis during their productive life and tested negative for California mastitis test (CMT) were kept in the mastitis tolerant group. PCR-SSCP technique was used to reveal the polymorphism in 256bp fragments of CXCR2 gene. The fragment was found to be monomorphic in the population studied and hence it can not be used as a candidate for selecting mastistis resistant cattle.

REFERENCES

  1. Bassam, B.J., Caetano-Anolles, G. and Gresshoff, P.M. (1991). Fast and sensitive silver staining of DNA in polyacrylamide gels. Analytical Biochem., 196: 80-83.
  2. Burvenich, C., Paape, M. J., Hill, A. E., Guidry, A. J., Miller, R. H., Heyneman, R., Kremer, W. D. and Brand, A. (1994). Role of the neutrophil leucocyte in the local and systemic reactions during experimentally induced E. coli mastitis in cows immediately after calving. Vet. Q., 16: 45–50.
  3. Kato H, Tsuchiya N and Tokunaga K. (2000). Single nucleotide polymorphisms in the coding regions of human CXC- chemokine receptors CXCR1, CXCR2, and CXCR3. Genes Immun., 1:330–337.
  4. Lahaussa, H., Pascal, R., Alain, C. and Celine, R. (2008). Identification and characterization of a new interleukin-8 receptor in bovine species. Molecular Immunology, 45: 1153-1164.
  5. Murphy, P. M. and Tiffany. H. L. (1991). Cloning of complementary DNA encoding a functional IL-8 receptor. Science., 253: 1278–1280.
  6. Orita, M., Iwahana, H. and Kanazawa, H. (1989). Detection of polymorphism of human DNA by gel electrophoresis as SSCP. Proc. National Acad. Sci. USA., 86: 2766-2770.
  7. Paape, M. J., Shafer-Weaver, K., Capuco, K. Van Oostvel. and Burvenich, C. (2000). Immune surveillance of mammary tissue by phagocytic cells. Adv. Exp. Med. Biol., 480: 259–277.
  8. Peveri, P., Walz, A., Dewald, B. and Baggiolini, M. (1988). A novel neutrophil-activating factor produced by human Polymorphonuclear leukocytes. J. Exp. Med., 181: 1547–1559.
  9. Podolin, P. L., Bolognese, B. J., Foley, J. J., Schmidt, D. B., Buckley, P. T., Widdowson, K. L., Jin, Q., White, J. R., Lee, J. M., Goodman, R. B., Hagen, T. R., Kajikawa, O., Marshall, L. A., Hay, D. W. and Sarau, H. M. (2002). A potent and selective nonpeptide antagonist of CXCR2 inhibits acute and chronic models of arthritis in the rabbit. J. Immunol., 169: 6435-6444.
  10. Renzoni, E., Lympany, P., Sestini, P., Pantelidis, P., Wells, A., Black, C., Welsh, K., Bunn, C., Knight, C., Foley, P. (2000). Distribution of novel polymorphisms of the interleukin-8 and CXC receptor 1 and 2 genes in systemic sclerosis and cryptogenic fibrosing alveolitis. Arthritis Rheum. 43:1633–1640.
  11. Sambrook, J. and Russell, D. W. (2001). Molecular cloning, a laboratory manual. 3rd edition. Cold spring harbor laboratory press, NY, USA.
  12. Schalm, O. W. and Noorlander, D. O. (1957) Experiments and observations leading to the development of California Mastitis Test. J. Am. Vet. Med. Assoc., 130: 199-204.
  13. Youngerman, S. M., Saxton, A. M., Oliver, S. P. and Pighetti, G. M. (2004) Association of CXCR2 polymorphisms with subclinical and clinical mastitis in dairy cattle. J. Dairy Sci., 87: 2442–2448.
Published In
Indian Journal of Animal Research
Article Metrics
Metrics Icon
0
Views
0
Citations
Reviewed By
Share Article
Download Article
In this Article
    Contact us
    Follow us

    volume 47 issue 4 (august 2013) : 335-339

    DISSECTING THE PARTIAL GENOMIC REGION OF CXCR TO CORRELATE WITH CMT IN VRINDAVANI CATTLE

    M
    Mahesh Shivanand Dige
    S
    S.P.S. Ahlawat
    B
    Bharat Bhusan*
    P
    Pushpendra Kumar
    A
    Anuj Chauhan
    B
    B. Inamdar
    L
    L.S. Kokate
    T
    Triveni Dutt1
    1Division of Animal Genetics and Breeding, Indian Veterinary Research Institute, Izatnagar, Bareilly-243 122, India

    • Submitted|

    • First Online |

    • doi

    Cite article:- Dige Shivanand Mahesh, Ahlawat S.P.S., Bhusan* Bharat, Kumar Pushpendra, Chauhan Anuj, Inamdar B., Kokate L.S., Dutt1 Triveni (2025). DISSECTING THE PARTIAL GENOMIC REGION OF CXCR TO CORRELATE WITH CMT IN VRINDAVANI CATTLE. Indian Journal of Animal Research. 47(4): 335-339. doi: .

    ABSTRACT

    Genetic markers associated with inflammatory responses during mastitis could aid in selection of cattle for mastitis resistance. The genomic region of 256 bp of CXCR2, a chemokine receptor required for neutrophil migration to infection sites was investigated in the current study to identify genetic polymorphism if any and to associate it with subclinical and clinical mastitis. Ninety five Vrindavani crossbred cows (42-mastitis tolerant and 53- with clinical mastitis) who completed at least two full lactations were taken for study. The cows which had never been affected by clinical mastitis during their productive life and tested negative for California mastitis test (CMT) were kept in the mastitis tolerant group. PCR-SSCP technique was used to reveal the polymorphism in 256bp fragments of CXCR2 gene. The fragment was found to be monomorphic in the population studied and hence it can not be used as a candidate for selecting mastistis resistant cattle.

    REFERENCES

    1. Bassam, B.J., Caetano-Anolles, G. and Gresshoff, P.M. (1991). Fast and sensitive silver staining of DNA in polyacrylamide gels. Analytical Biochem., 196: 80-83.
    2. Burvenich, C., Paape, M. J., Hill, A. E., Guidry, A. J., Miller, R. H., Heyneman, R., Kremer, W. D. and Brand, A. (1994). Role of the neutrophil leucocyte in the local and systemic reactions during experimentally induced E. coli mastitis in cows immediately after calving. Vet. Q., 16: 45–50.
    3. Kato H, Tsuchiya N and Tokunaga K. (2000). Single nucleotide polymorphisms in the coding regions of human CXC- chemokine receptors CXCR1, CXCR2, and CXCR3. Genes Immun., 1:330–337.
    4. Lahaussa, H., Pascal, R., Alain, C. and Celine, R. (2008). Identification and characterization of a new interleukin-8 receptor in bovine species. Molecular Immunology, 45: 1153-1164.
    5. Murphy, P. M. and Tiffany. H. L. (1991). Cloning of complementary DNA encoding a functional IL-8 receptor. Science., 253: 1278–1280.
    6. Orita, M., Iwahana, H. and Kanazawa, H. (1989). Detection of polymorphism of human DNA by gel electrophoresis as SSCP. Proc. National Acad. Sci. USA., 86: 2766-2770.
    7. Paape, M. J., Shafer-Weaver, K., Capuco, K. Van Oostvel. and Burvenich, C. (2000). Immune surveillance of mammary tissue by phagocytic cells. Adv. Exp. Med. Biol., 480: 259–277.
    8. Peveri, P., Walz, A., Dewald, B. and Baggiolini, M. (1988). A novel neutrophil-activating factor produced by human Polymorphonuclear leukocytes. J. Exp. Med., 181: 1547–1559.
    9. Podolin, P. L., Bolognese, B. J., Foley, J. J., Schmidt, D. B., Buckley, P. T., Widdowson, K. L., Jin, Q., White, J. R., Lee, J. M., Goodman, R. B., Hagen, T. R., Kajikawa, O., Marshall, L. A., Hay, D. W. and Sarau, H. M. (2002). A potent and selective nonpeptide antagonist of CXCR2 inhibits acute and chronic models of arthritis in the rabbit. J. Immunol., 169: 6435-6444.
    10. Renzoni, E., Lympany, P., Sestini, P., Pantelidis, P., Wells, A., Black, C., Welsh, K., Bunn, C., Knight, C., Foley, P. (2000). Distribution of novel polymorphisms of the interleukin-8 and CXC receptor 1 and 2 genes in systemic sclerosis and cryptogenic fibrosing alveolitis. Arthritis Rheum. 43:1633–1640.
    11. Sambrook, J. and Russell, D. W. (2001). Molecular cloning, a laboratory manual. 3rd edition. Cold spring harbor laboratory press, NY, USA.
    12. Schalm, O. W. and Noorlander, D. O. (1957) Experiments and observations leading to the development of California Mastitis Test. J. Am. Vet. Med. Assoc., 130: 199-204.
    13. Youngerman, S. M., Saxton, A. M., Oliver, S. P. and Pighetti, G. M. (2004) Association of CXCR2 polymorphisms with subclinical and clinical mastitis in dairy cattle. J. Dairy Sci., 87: 2442–2448.
    In this Article
      Contact us
      Follow us
      Published In
      Indian Journal of Animal Research

      Editorial Board

      View all (0)