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Indian Journal of Animal Research, volume 44 issue 2 (june 2010) : 131 134

MOLECULAR CLONING OF A DNA FRAGMENT ENCODING 34.9 KDA CARBOXY TERMINAL PPE PROTEIN OF MYCOBACTERIUMAVIUMSUBSP. PARATUBERCULOSIS IN E.COLI *

R. Deb, P.P. Goswami, V.K. Saxena, N.S. Prasad

Affiliations

¹Department of Animal Biotechnology Indian Veterinary Research Institute. Izatnagar, 243122. India

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Cite article:- Deb R., Goswami P.P., Saxena V.K., Prasad N.S. (2024). MOLECULAR CLONING OF A DNA FRAGMENT ENCODING 34.9 KDA CARBOXY TERMINAL PPE PROTEIN OF MYCOBACTERIUMAVIUMSUBSP. PARATUBERCULOSIS IN E.COLI *. Indian Journal of Animal Research. 44(2): 131 134. doi: .

ABSTRACT

Mycobacterium ar/ium ssp. Paratuberculosis (Map) is the causative agent of johne's disease in animals. A PPE (Proline Proline Glutamic acid rich) antigen encoding 34.9 kDa hypothetical protein of M avium subsp. ParatuberclIlosiswas cloned and sequenced. Sequence analysis revealed that no other published sequences having significant homology with that PPE gene .which elucidated that the PPE 34.9 kDa could be used as diagnostic antigen.

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MOLECULAR CLONING OF A DNA FRAGMENT ENCODING 34.9 KDA CARBOXY TERMINAL PPE PROTEIN OF MYCOBACTERIUMAVIUMSUBSP. PARATUBERCULOSIS IN E.COLI *

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