MOLECULAR CLONING OF A DNA FRAGMENT ENCODING 34.9 KDA CARBOXY TERMINAL PPE PROTEIN OF MYCOBACTERIUMAVIUMSUBSP. PARATUBERCULOSIS IN E.COLI *

Article Id: ARCC1226 | Page : 131 134
Citation :- MOLECULAR CLONING OF A DNA FRAGMENT ENCODING 34.9 KDA CARBOXY TERMINAL PPE PROTEIN OF MYCOBACTERIUMAVIUMSUBSP. PARATUBERCULOSIS IN E.COLI *.Indian Journal Of Animal Research.2010.(44):131 134
R. Deb, P.P. Goswami, V.K. Saxena and N.S. Prasad
Address : Department of Animal Biotechnology Indian Veterinary Research Institute. Izatnagar, 243122. India

Abstract

Mycobacterium ar/ium ssp. Paratuberculosis (Map) is the causative agent of johne's disease in animals. A PPE (Proline Proline Glutamic acid rich) antigen encoding 34.9 kDa hypothetical protein of M avium subsp. ParatuberclIlosiswas cloned and sequenced. Sequence analysis revealed that no other published sequences having significant homology with that PPE gene .which elucidated that the PPE 34.9 kDa could be used as diagnostic antigen.

Keywords

PPE 34.9 kDa Mycobacterium avium ssp. Paratuberculosis FJ032182

References

  1. Bannantine, J.P eta/. (2004). Am. Sodet. Microbjol .. 42: 106-114
  2. Choudhary. R. K. S. eta/., (2003). Infect. Immun. 71:6338-6343.
  3. Cole, S T eta/.. (1998).Nature393: 537-544.
  4. Dillion. 0, C. etal.lnfect.Immun.(1999) 67:2941-2950
  5. Fleischmann, R. D, eta!., (2002). J Bacteriol 184: 5479-5490.
  6. Huntley. J.F. et al. (2005) Infect, Immun., 73: 6877-6884.
  7. Mullerad, J. et a!., (2002a) FEMS Immunol. Med. Microbio!', 34: 81-88.
  8. Mullerad, J. eta!., (2002b) Mo!. Microbio!' Immuno/.. 190: 179-187.
  9. Mullerad, J. etal.. (2003) Microbiol. Pathogenesis, 34: 81-90.
  10. Okkels, L M.. et aI., (2003). Infect. Immun. 71: 6116-6123.
  11. Portillo, PD. eta/., (1991) Plasmid, 39: 100-113.
  12. Sambrook, J. and Russel. D. W. (2001) Molecular cloning: A laboratory manual3,d edn. LoldSpring Harbor Laboratory Press. Cold spring Harbor;NY.

Global Footprints