volume 47 issue 2 (april 2013) : 164-167
GENETIC VARIABILITY OF BOLA-DRB3.2 GENE IN KENKATHA BREED OF CATTLE ASSESSED BY PCR-SSCP
1Animal Biotechnology Centre,
MPPCVV, Jabalpur 482 004, India
Published In
Indian Journal of Animal Research
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volume 47 issue 2 (april 2013) : 164-167
GENETIC VARIABILITY OF BOLA-DRB3.2 GENE IN KENKATHA BREED OF CATTLE ASSESSED BY PCR-SSCP
1Animal Biotechnology Centre,
MPPCVV, Jabalpur 482 004, India
ABSTRACT
Present study was conducted to investigate BoLA (Bovine leucocyte antigen) gene polymorphism in indigenous draft Kenkatha (n=52) breed of cattle at DRB3.2 loci by PCR-SSCP (Single strand conformational polymorphism). This BoLA region encodes cell surface glycoprotein, which binds to foreign antigens of T lymphocytes that play central role in the induction and regulation of adaptive immunity. A PCR-SSCP of 284bp amplicon of BoLA-DRB3.2 gene revealed 13 SSCP patterns. This suggests that exon 2 region of BoLA-DRB3 is highly polymorphic in nature. This may be due to natural selection on a BoLA allele pool, leading to the development of resistance to local infectious diseases.
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In this Article
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Published In
Indian Journal of Animal Research