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Current IssueEditorial BoardOnline First ArticlesArchive

volume 47 issue 2 (april 2013) : 126-131

EFFECT OF DIFFERENT YEASTS ON SELENOMONAS RUMINANTIUM UTILIZING LACTATE IN VITRO

M
Miao Long1
2
2
J
Jing Li3
X
Xia Qin2
Y
Yiming Li1
P
Peng Li1
G
Guo-Wen Liu2*
X
Xiaobing Li2*
1College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 110866, P. R. China

  • Submitted|

  • First Online |

  • doi

Cite article:- Long1 Miao, 2, Li3 Jing, Qin2 Xia, Li1 Yiming, Li1 Peng, Liu2* Guo-Wen, Li2* Xiaobing (2025). EFFECT OF DIFFERENT YEASTS ON SELENOMONAS RUMINANTIUM UTILIZING LACTATE IN VITRO. Indian Journal of Animal Research. 47(2): 126-131. doi: .

ABSTRACT

The objective of this study was to determine the effects of six yeast (Saccharomyces cerivisiae, Candida tropicalis, Pichia burtonii, Candida utilis, Rhodothece glutinis, Geotrichum candidum) on lactate utilization by the predominant ruminal bacterium Selenomonas ruminantium(S. ruminantium). Four different concentration of these yeasts (6g/l, 8g/l, 10g/l, 12g/l) and non-yeast control group were set. The pH and the concentration of lactate, acetate, and propionate in the culture fluids were detected at 12h after adding these different yeasts. The results indicated that lactate uptake can be stimulated by all the six yeast at all of six concentration. When the added yeast concentration was 10g/l-12g/l, lactate uptake by S. ruminantium was achieved the greatest promote. The six yeasts also increased the production of acetate and propionate and the ratio of propionate to acetate. Among the six yeasts, Candida tropicalis, Geotrichum candidum and Candida utilis affecting on above indexes were better than Saccharomyces cervisiae, Pichia burtonii and Rhodothece glutinis.

REFERENCES

  1. Arcos-Garcia, J.L., Castrejon, F.A., Mendoza, G.D. and Perez-Gavilan E.P., (2000). Effect of two commercial yeast cultures with Saccharomyces cerevisiae on ruminal fermentation and digestion in sheep fed sugar cane tops. Livest. Prod. Sci., 63: 153-157.
  2. ATCC. (1990). American Type Culture Collection, Catalogue of Yeasts (18th Ed.). Jong S. C. and Edwards (Ed.) M. J. American Type Culture Collection, Rockville, MD.
  3. Caldwell, D.R. and Bryant M.P., (1966). Medium without rumen fluid for non-selective enumeration and isolation of rumen bacteria. Appl Microbiol 14: 794-801
  4. Doležal, P., Doležal, J. and J Trinacty, (2005).The effect of Saccharomyces cerevisiae on ruminal fermentation in dairy cows. Czech. J. Anim. Sci., 50: 503-510
  5. Enemark J.M.D. (2008). The monitoring, prevention and treatment of sub-acute ruminal acidosis (SARA): A review. Vet. J., 176: 32–43
  6. Guedes, C.M., Goncalves D., Rodrigues M.A.M., and Dias. da Silva. A., (2008). Effects of a Saccharomyces cerevisiae yeast on ruminal fermentation and fibre degradation of maize silages in cows. Anim Feed Sci Technol 145: 27– 40.
  7. Martin, C., Brossard, L. and M. Doreau, (2006a). M´ecanismes d’apparition de l’acidose ruminale latente et cons´equences physiopathologiques et zootechniques. Inra. Prod. Anim., 19: 93–108.
  8. Newbold, C.J., Wallace, R.J., Chen, X.B. and F.M. McIntosh, 1995. Different strains of Saccharomyces cerevisae differ in their effects on ruminal bacterial numbers in vitro and in sheep. J.Anim. Sci., 73: 1811–1818
  9. Nisbet, D. J., and S.A. Martin,(1993). Effects of fumarate, Lmalate, and an Aspergillus oryzae fermentation extract on Dlactate utilization by the ruminal bacterium Selenomonas ruminantium. Curr. Microbiol., 26: 133-136.
  10. Nisbet, D.J. and Martin S.A. , (1991). Effect of a Saccharomyces cerevisiae culture on lactate utilization by the ruminal bacterium Selenomonas ruminantium. J. Anim. Sci., 69: 4628-4633.
  11. Nisbet, D.J., and Martin S.A., (1990). Effect of dicarboxylic acids and Aspergillus oryzae fermentation extract on lactate uptake by the ruminal bacterium Selenomonas ruminantium. Appl. Environ. Microbiol., 56: 3515- 3518
  12. Russell JB and Hespell R.B., (1981) . Microbial rumen fermentntion. J. Dairy Sci. 64.1153.
  13. Scheifinger, C.C., Latham, M.J. and M.J. Wolin, (1975). Relationship of lactate dehydrogenase specificity and growth rate to lactate metabolism by Selenomonas ruminantiurn. Appl. Microbiol., 30(6):916-921
  14. Waldrip, H.M., and Martin S.A., (1993). Effects of an Aspergillus oryzae fermentation extract and other factors on lactate utilization by the ruminal bacterium Megasphaera elsdenii. J.Anim. Sci., 71: 2770-2776.
  15. Wallace, R. J. and Newbold C. J., (1992). Probiotics for ruminants. In Probiotics: The Scient$c Basis, [R. Fuller, editor]. London: Chapman & Hall pp. 317-353.
  16. Williams, P.E., Tait. C.A, Innes, G.M., Newbold, C. J. (1991): Effects of the inclusion of yeast culture (Saccharomycescerevisiae plus growth medium) in the diet of dairy cows on milk yield and forage degradation and fermentation patterns in the rumen of steers. J Anim Sci 69: 3016–3026
  17. Wohlt J.E., Finkelstein A.D., and Chung C.H. , (1991). Yeast culture to improve intake, nutrient digestibility, and performance by dairy cattle during early lactation. J. Dairy. Sci.,74: 1395-1400
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    volume 47 issue 2 (april 2013) : 126-131

    EFFECT OF DIFFERENT YEASTS ON SELENOMONAS RUMINANTIUM UTILIZING LACTATE IN VITRO

    M
    Miao Long1
    2
    2
    J
    Jing Li3
    X
    Xia Qin2
    Y
    Yiming Li1
    P
    Peng Li1
    G
    Guo-Wen Liu2*
    X
    Xiaobing Li2*
    1College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 110866, P. R. China

    • Submitted|

    • First Online |

    • doi

    Cite article:- Long1 Miao, 2, Li3 Jing, Qin2 Xia, Li1 Yiming, Li1 Peng, Liu2* Guo-Wen, Li2* Xiaobing (2025). EFFECT OF DIFFERENT YEASTS ON SELENOMONAS RUMINANTIUM UTILIZING LACTATE IN VITRO. Indian Journal of Animal Research. 47(2): 126-131. doi: .

    ABSTRACT

    The objective of this study was to determine the effects of six yeast (Saccharomyces cerivisiae, Candida tropicalis, Pichia burtonii, Candida utilis, Rhodothece glutinis, Geotrichum candidum) on lactate utilization by the predominant ruminal bacterium Selenomonas ruminantium(S. ruminantium). Four different concentration of these yeasts (6g/l, 8g/l, 10g/l, 12g/l) and non-yeast control group were set. The pH and the concentration of lactate, acetate, and propionate in the culture fluids were detected at 12h after adding these different yeasts. The results indicated that lactate uptake can be stimulated by all the six yeast at all of six concentration. When the added yeast concentration was 10g/l-12g/l, lactate uptake by S. ruminantium was achieved the greatest promote. The six yeasts also increased the production of acetate and propionate and the ratio of propionate to acetate. Among the six yeasts, Candida tropicalis, Geotrichum candidum and Candida utilis affecting on above indexes were better than Saccharomyces cervisiae, Pichia burtonii and Rhodothece glutinis.

    REFERENCES

    1. Arcos-Garcia, J.L., Castrejon, F.A., Mendoza, G.D. and Perez-Gavilan E.P., (2000). Effect of two commercial yeast cultures with Saccharomyces cerevisiae on ruminal fermentation and digestion in sheep fed sugar cane tops. Livest. Prod. Sci., 63: 153-157.
    2. ATCC. (1990). American Type Culture Collection, Catalogue of Yeasts (18th Ed.). Jong S. C. and Edwards (Ed.) M. J. American Type Culture Collection, Rockville, MD.
    3. Caldwell, D.R. and Bryant M.P., (1966). Medium without rumen fluid for non-selective enumeration and isolation of rumen bacteria. Appl Microbiol 14: 794-801
    4. Doležal, P., Doležal, J. and J Trinacty, (2005).The effect of Saccharomyces cerevisiae on ruminal fermentation in dairy cows. Czech. J. Anim. Sci., 50: 503-510
    5. Enemark J.M.D. (2008). The monitoring, prevention and treatment of sub-acute ruminal acidosis (SARA): A review. Vet. J., 176: 32–43
    6. Guedes, C.M., Goncalves D., Rodrigues M.A.M., and Dias. da Silva. A., (2008). Effects of a Saccharomyces cerevisiae yeast on ruminal fermentation and fibre degradation of maize silages in cows. Anim Feed Sci Technol 145: 27– 40.
    7. Martin, C., Brossard, L. and M. Doreau, (2006a). M´ecanismes d’apparition de l’acidose ruminale latente et cons´equences physiopathologiques et zootechniques. Inra. Prod. Anim., 19: 93–108.
    8. Newbold, C.J., Wallace, R.J., Chen, X.B. and F.M. McIntosh, 1995. Different strains of Saccharomyces cerevisae differ in their effects on ruminal bacterial numbers in vitro and in sheep. J.Anim. Sci., 73: 1811–1818
    9. Nisbet, D. J., and S.A. Martin,(1993). Effects of fumarate, Lmalate, and an Aspergillus oryzae fermentation extract on Dlactate utilization by the ruminal bacterium Selenomonas ruminantium. Curr. Microbiol., 26: 133-136.
    10. Nisbet, D.J. and Martin S.A. , (1991). Effect of a Saccharomyces cerevisiae culture on lactate utilization by the ruminal bacterium Selenomonas ruminantium. J. Anim. Sci., 69: 4628-4633.
    11. Nisbet, D.J., and Martin S.A., (1990). Effect of dicarboxylic acids and Aspergillus oryzae fermentation extract on lactate uptake by the ruminal bacterium Selenomonas ruminantium. Appl. Environ. Microbiol., 56: 3515- 3518
    12. Russell JB and Hespell R.B., (1981) . Microbial rumen fermentntion. J. Dairy Sci. 64.1153.
    13. Scheifinger, C.C., Latham, M.J. and M.J. Wolin, (1975). Relationship of lactate dehydrogenase specificity and growth rate to lactate metabolism by Selenomonas ruminantiurn. Appl. Microbiol., 30(6):916-921
    14. Waldrip, H.M., and Martin S.A., (1993). Effects of an Aspergillus oryzae fermentation extract and other factors on lactate utilization by the ruminal bacterium Megasphaera elsdenii. J.Anim. Sci., 71: 2770-2776.
    15. Wallace, R. J. and Newbold C. J., (1992). Probiotics for ruminants. In Probiotics: The Scient$c Basis, [R. Fuller, editor]. London: Chapman & Hall pp. 317-353.
    16. Williams, P.E., Tait. C.A, Innes, G.M., Newbold, C. J. (1991): Effects of the inclusion of yeast culture (Saccharomycescerevisiae plus growth medium) in the diet of dairy cows on milk yield and forage degradation and fermentation patterns in the rumen of steers. J Anim Sci 69: 3016–3026
    17. Wohlt J.E., Finkelstein A.D., and Chung C.H. , (1991). Yeast culture to improve intake, nutrient digestibility, and performance by dairy cattle during early lactation. J. Dairy. Sci.,74: 1395-1400
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