IDENTIFICATION OF CARD-15 GENE POLYMORPHISM IN SAHIWAL CATTLE

Article Id: ARCC101 | Page : 83-86
Citation :- IDENTIFICATION OF CARD-15 GENE POLYMORPHISM IN SAHIWAL CATTLE.Indian Journal Of Animal Research.2013.(47):83-86
N.P. Soumya, I.D. Gupta, Archana Verma, K.N. Raja, Inderasen Chauhan and M.V. Chaudhari
Address : Molecular Genetics Lab, Dairy Cattle Breeding Division, National Dairy Research Institute, Karnal-132 001, India

Abstract

Caspase Activating Recruitment Domain-15 (CARD-15) gene plays an important role in initiation of inflammatory reactions and subsequent immune response. They have been identified as susceptibility loci for inflammatory bowel disease in humans and are, therefore, suitable candidate gene for inflammatory disease resistance in cattle. As 1988 bp long exon 12 was found to be highly polymorphic in Bos taurus, this study was conducted to explore polymorphism in exon 12 of CARD-15 gene in Bos indicus. Genomic DNA were extracted from 162 lactating Sahiwal cattle maintained at National Dairy Research Institute (NDRI), Karnal (Haryana) and population was screened using PCR-RFLP with PvuII and SacI restriction enzymes. Five sets of primers were used to amplify five overlapping contigs of exon 12 i.e. 12.1 to 12.5 (578, 619, 641, 509 and 588 bp). PvuII RE exhibited AA (1.9%), AB (91.9%) and BB (6.2%) genotypes in contig 12.5, whereas, SacI RE revealed only two, AA (46.9%) and AB (53.1%) polymorphic patterns in contig 12.2. Seven SNPs [240 (T>C), 820 (C>T), 1487 (A>G), 1641 (A>G), 1682 (G>A), 1685 (T>A) and 1982 (C>T)] were found in the complete sequence of exon 12 of CARD-15 gene of Sahiwal cattle. It is concluded that there are several SNPs in 3’ UTR of Bos indicus as compared to that of Bos taurus.

Keywords

CARD 15 gene PCR-RFLP SNPs Sahiwal.

References

  1. Mathew, C. G. and Lewis, C. M. (2004). Genetics of Inflammatory Bowel Disease: Progress and prospects. Human Mol. Genet., 13: 161-168.
  2. Miceli-Richard, C., Lesage, S., Rybojad, M., Prieur, A. M., Manouvrier-Hanu, S., Häfner, R., Chamaillard, M., Zouali, H., Thomas, G. and Hugot, J. P. (2001). CARD15 mutations in Blau syndrome. Nature Genetics, 29: 19-20.
  3. Ogura, Y., Inohara, N., Benito, A., Chen, F. F., Yamaoka, S. and Nunez, G. (2001). NOD2, a NOD1/Apaf-1 family member that is restricted to monocytes and activates NF-kappaB. The J. Biol. Chem., 276: 4812-18.
  4. Pant, S. D., Schenkel, F. S., Leyva-Baca, I., Sharma, B. S. and Karrow, N. A., (2007). Identification of single nucleotide polymorphisms in bovine CARD15 and their associations with health and production traits in Canadian Holsteins. BMC Genomics, 8: 421.
  5. Sambrook, J. and Russell, D. W. (1989). Preparation and analysis of eukaryotic DNA. In: Molecular Cloning: A Laboratory Manual.3rd Edition. Cold Spring Harbor Laboratory Press, New York: 6.1-6.62.
  6. Taylor, K. H. (2004). Genetic analyses of bovine CARD15, a putative disease resistance gene. Ph.D. Thesis Office of Graduate Studies of Texas A&M University.
  7. van Duist, M. M., Albrecht, M., Podswiadek, M., Giachino, D., Lengauer, T., Punzi, L. and Marchi, M. D. (2005). A new CARD15 mutation in Blau syndrome. European J. Human Gen., 13: 742-747.

Global Footprints