volume 47 issue 1 (february 2013) : 83-86
IDENTIFICATION OF CARD-15 GENE POLYMORPHISM IN SAHIWAL CATTLE
1Molecular Genetics Lab, Dairy Cattle Breeding Division,
National Dairy Research Institute, Karnal-132 001, India
Published In
Indian Journal of Animal Research
Article Metrics
0
Views
0
Citations
Reviewed By
In this Article
APC
APC cover the cost of turning a manuscript into a published manuscript through peer-review process, editorial work as well as the cost of hosting, distributing, indexing and promoting the manuscript.
Publish With US
Submit your manuscript through user friendly platform and acquire the maximum impact for your research by publishing with ARCC Journals.
Become a Reviewer/Member
Join our esteemed reviewers panel and become an editorial board member with international experts in the domain of numerous specializations.
Open Access
Filling the gap between research and communication ARCC provide Open Access of all journals which empower research community in all the ways which is accessible to all.
Products and Services
We provide prime quality of services to assist you select right product of your requirement.
Support and Policies
Finest policies are designed to ensure world class support to our authors, members and readers. Our efficient team provides best possible support for you.
volume 47 issue 1 (february 2013) : 83-86
IDENTIFICATION OF CARD-15 GENE POLYMORPHISM IN SAHIWAL CATTLE
1Molecular Genetics Lab, Dairy Cattle Breeding Division,
National Dairy Research Institute, Karnal-132 001, India
ABSTRACT
Caspase Activating Recruitment Domain-15 (CARD-15) gene plays an important role in initiation of inflammatory reactions and subsequent immune response. They have been identified as susceptibility loci for inflammatory bowel disease in humans and are, therefore, suitable candidate gene for inflammatory disease resistance in cattle. As 1988 bp long exon 12 was found to be highly polymorphic in Bos taurus, this study was conducted to explore polymorphism in exon 12 of CARD-15 gene in Bos indicus. Genomic DNA were extracted from 162 lactating Sahiwal cattle maintained at National Dairy Research Institute (NDRI), Karnal (Haryana) and population was screened using PCR-RFLP with PvuII and SacI restriction enzymes. Five sets of primers were used to amplify five overlapping contigs of exon 12 i.e. 12.1 to 12.5 (578, 619, 641, 509 and 588 bp). PvuII RE exhibited AA (1.9%), AB (91.9%) and BB (6.2%) genotypes in contig 12.5, whereas, SacI RE revealed only two, AA (46.9%) and AB (53.1%) polymorphic patterns in contig 12.2. Seven SNPs [240 (T>C), 820 (C>T), 1487 (A>G), 1641 (A>G), 1682 (G>A), 1685 (T>A) and 1982 (C>T)] were found in the complete sequence of exon 12 of CARD-15 gene of Sahiwal cattle. It is concluded that there are several SNPs in 3’ UTR of Bos indicus as compared to that of Bos taurus.
KEYWORDS
REFERENCES
- Mathew, C. G. and Lewis, C. M. (2004). Genetics of Inflammatory Bowel Disease: Progress and prospects. Human Mol. Genet., 13: 161-168.
- Miceli-Richard, C., Lesage, S., Rybojad, M., Prieur, A. M., Manouvrier-Hanu, S., Häfner, R., Chamaillard, M., Zouali, H., Thomas, G. and Hugot, J. P. (2001). CARD15 mutations in Blau syndrome. Nature Genetics, 29: 19-20.
- Ogura, Y., Inohara, N., Benito, A., Chen, F. F., Yamaoka, S. and Nunez, G. (2001). NOD2, a NOD1/Apaf-1 family member that is restricted to monocytes and activates NF-kappaB. The J. Biol. Chem., 276: 4812-18.
- Pant, S. D., Schenkel, F. S., Leyva-Baca, I., Sharma, B. S. and Karrow, N. A., (2007). Identification of single nucleotide polymorphisms in bovine CARD15 and their associations with health and production traits in Canadian Holsteins. BMC Genomics, 8: 421.
- Sambrook, J. and Russell, D. W. (1989). Preparation and analysis of eukaryotic DNA. In: Molecular Cloning: A Laboratory Manual.3rd Edition. Cold Spring Harbor Laboratory Press, New York: 6.1-6.62.
- Taylor, K. H. (2004). Genetic analyses of bovine CARD15, a putative disease resistance gene. Ph.D. Thesis Office of Graduate Studies of Texas A&M University.
- van Duist, M. M., Albrecht, M., Podswiadek, M., Giachino, D., Lengauer, T., Punzi, L. and Marchi, M. D. (2005). A new CARD15 mutation in Blau syndrome. European J. Human Gen., 13: 742-747.
Disclaimer :
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Copyright :
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
In this Article
APC
APC cover the cost of turning a manuscript into a published manuscript through peer-review process, editorial work as well as the cost of hosting, distributing, indexing and promoting the manuscript.
Publish With US
Submit your manuscript through user friendly platform and acquire the maximum impact for your research by publishing with ARCC Journals.
Become a Reviewer/Member
Join our esteemed reviewers panel and become an editorial board member with international experts in the domain of numerous specializations.
Open Access
Filling the gap between research and communication ARCC provide Open Access of all journals which empower research community in all the ways which is accessible to all.
Products and Services
We provide prime quality of services to assist you select right product of your requirement.
Support and Policies
Finest policies are designed to ensure world class support to our authors, members and readers. Our efficient team provides best possible support for you.
Published In
Indian Journal of Animal Research