PHYLOGENETIC ANALYSIS OF EXON II OF MELATONIN RECEPTOR 1A (MTNR1A) GENE IN MERINO CROSS EWES IN KASHMIR

Article Id: ARCC097 | Page : 66-69
Citation :- PHYLOGENETIC ANALYSIS OF EXON II OF MELATONIN RECEPTOR 1A (MTNR1A) GENE IN MERINO CROSS EWES IN KASHMIR.Indian Journal Of Animal Research.2013.(47):66-69
T. Ahmad*, T.A.S. Ganai, A.K. Pandey1 and A. Magotra2 tavsief@gmail.com
Address : Division of Animal Genetics and Breeding, SKUAST-K, Srinagar-190 006, India

Abstract

The seasonality of sheep reproduction is a major limiting factor in round the year lamb production under temperate climate. The objective of this study was to analyse allelic variants of exon II of melatonin receptor (MTNR1A) gene in Merino cross ewes by phylogenetic analysis. The genotypes of the MTNR1A were determined by PCR-RFLP. Genomic DNA was extracted from 49 Merino cross ewes, PCR reaction was carried out and PCR products were digested by the Mnl1 restriction enzyme and placed into M/M, M/m or m/m genotypes. Sequencing of the representative samples showing different RFLP patterens were got done both in forward as well as reverse direction. The obtained nucleotide sequences of different alleles of exon II fragment of the gene were analysed to generate phylogenetic relationship, the homology (percentage similarity) and the relative distance (divergence) with sheep (Acc. No’s U14109, AF045219), goat (Acc. No AF419334), cattle (Acc. No EU716174), buffalo (Acc. No GU817415) and human (Acc. No AB029933). The results confirmed that the diversity in the amplified fragment is in consonance with the phylogenty of species. This study also provided evidence that Merino cross have variability in MTNR1A locus. Therefore, this locus can be used as a marker for the reproduction trait in selection programs.

Keywords

Ewes MnlI MTNR1A gene PCR-RFLP Seasonal reproduction Sequencing.

References

  1. Barrett, P., Conway, S., Jockers, R., Strosberg, A.D., Guardiola-Lemaitre, B., Delagrange, P. and Morgan, P.J., (1997). Cloning and functional analysis of a polymorphic variant of the ovine Mel 1a melatonin receptor. Biochim. Biophys. Acta 1356 : 299-307.
  2. Carcangiu,V., Mura,M.C., Pazzola,M., Marchi,B., Vacca,G.M. and Luridiana,S. (2010). Bubalus bubalis melatonin receptor type 1A (MTNR1A) gene, partial cds (Acc. No GU817415). Direct Submission to NCBI
  3. Dreger,D.L. and Schmutz,S.M. (2008). Characterization of MTNR1A in cattle. Submitted to the EMBL/GenBank/ DDBJ databases.
  4. Dubocovich, M.L., Rivera-Bermudez, M.A., Gerdin, M.J. and Masan, M.I. (2003). Molecular pharmacology, regulation and function of mammalian melatonin receptors. Front. Biosci. 8 : 1093-1108.
  5. Ebisawa,T., Kajimura,N., Uchiyama,M., Katoh,M., Sekimoto,M., Watanabe,T., Ozeki,Y., Ikeda,M., Jodoi,T., Sugishita,M., Iwase,T., Kamei,Y., Kim,K., Shibui,K., Kudo,Y., Yamada,N., Toyoshima,R., Okawa,M., Takahashi,K. and Yamauchi,T (1999). Alleic variants of human melatonin 1a receptor: function and prevalence in subjects with circadian rhythm sleep disorders. Biochem. Biophys. Res. Commun. 262 : 832-837
  6. Karsch, F.J., Bittman, E.L., Foster, D.L., Goodman, R.L., Legan, S.J. and Robinson, J.E. (1984). Neuroendocrine basis of seasonal reproduction. Recent Prog. Horm. Res. 40 :185-232.
  7. Karsch, F.J., Malpaux, B., Wayne, N.L. and Robinson, J.E. (1988). Characteristics of the melatonin signal that provide the photoperiodic code for timing seasonal reproduction in the ewe. Reprod. Nutr. Dev. 28 : 459-472.
  8. Messer, L.A., Wang, L., Christopher, K., Tuggle, C.K., Yerle, M., Chardon, P., Pomp, D., Womack, J.E., Barendse, W., Crawford, A.M., Notter, D.R. and Rothschild, M.F. (1997). Maping of the melatonin receptor 1a (MTNR1a) gene in pigs, sheep and Cattle. Mamm. Genome 8 : 368-370.
  9. Migaud, M., Gavet, S. and Pelletier, J. (2002). Partial cloning and polymorphism of the melatonin 1a (Mel1a) receptor gene in two breeds of goat with different reproductive seasonality. Reproduction 124 : 59-64.
  10. Nosjean, O., Ferr, M., Coge, F., Beauverger, P., Henlin, J.M., Lefoulon, F., Fauchere, J.L., Delagrange, P., Canet, E. and Boutin, J.A. (2000). Identification of the melatonin-binding site MT3 as the quinone reductase 2. J. Biol. Chem. 275 : 31311-31317.
  11. Reppert, S.M., Weaver, D.R. and Ebisawa, T. (1994). Cloning and characterization of a mammalian melatonin receptor that mediates reproductive and circadian responses. Neuron 13 : 1177-1185.
  12. Reppert, S.M., Weaver, D.R. and Godson, C. (1996). Melatonin receptors step into the light: cloning and classification of subtypes .Trends in Pharmacol. Scie. 17 : 100-102.
  13. Sambrook, J. and Russel, D.W. (2001).Molecular Cloning, A Laboratory Manual. 3rd edition. Cold Spring Harbour Laboratory Press, New York USA.
  14. Weaver, D.R., Liu, C. and Reppert, S.M. (1996). Nature’s knock-out: the Mel1b receptor is not necessary for reproductive and circadian responses to melatonin in Siberian hamsters. Mol. Endocrinol. 10 : 1478-1487.

Global Footprints