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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 35 issue 1-2 (march-june 2020) : 33-38,   Doi: 10.18805/BKAP199

In-vitro evaluation of six bio-product against coriander stem gall disease

M
Munish Leharwan
M
Meenu Gupta
S
Sanjeev Leharwan
1<div style="text-align: justify;">Department of Plant Pathology, Yashwant Singh Parmar University of Horticulture and Forestry,&nbsp;Nauni-173 230, Solan, Himachal Pradesh.</div>

  • Submitted22-05-2020|

  • Accepted18-07-2020|

  • First Online 10-09-2020|

  • doi 10.18805/BKAP199

Cite article:- Leharwan Munish, Gupta Meenu, Leharwan Sanjeev (2020). In-vitro evaluation of six bio-product against coriander stem gall disease. Bhartiya Krishi Anusandhan Patrika. 35(1): 33-38. doi: 10.18805/BKAP199.

ABSTRACT

Stem gall of coriander caused by Protomyces macrosporus Unger. is a highly devastating seed and soil borne pathogen in coriander and has emerged as one of the major disease causing substantial quantitative and qualitative losses. In the present studies, efficacy of six bio-products, namely, Neemazal, cow urine, butter milk, fresh milk, beejamrit and jeevamrit was screened against mycelial growth inhibition of P. macrosporus under in vitro conditions. Extracts of these bio-products were evaluated at three different concentrations (i.e 10, 20 and 30%) by poison food technique. Results revealed that that among the various bio-products evaluated against test pathogen, irrespective of concentration tested, all the bio-products resulted in significant inhibition in mycelial growth of test pathogen with neemazal giving the complete inhibition [100.00 % (90.00)] followed by fresh milk [30.66 % (28.44)] and butter milk [26.55 % (25.99)], though these treatments were statistically different from one another. Different concentrations (10 to 30 %) of bio-products, irrespective of the treatments showed that 30 per cent concentration of all bio-products was most efficacious in inhibiting the mycelial growth of the test pathogen giving 69.95 per cent (36.77) growth inhibition. This was followed 20 and 10 per cent concentration of bio-products resulting in 55.58 per cent (32.49) and 45.53 per cent (16.30) growth inhibition, respectively. The result regarding evaluation of bio-products under in vitro conditions against the P. macrosporus showed that Neemazal treatment was most effective at all concentrations and completely inhibited the test fungus followed by 30 per cent fresh milk resulting in the mycelial growth inhibition of 51.60 per cent.

REFERENCES

  1. Bhat N.M. and Srivastava L.S. (2003). Evaluation of some fungicides and neem formulations against six soil-borne pathogens and three Trichoderma spp. In vitro. Plant Disease Research. 18:56-59.
  2. Bhat S., Kaushal P., Kaur M. and Sharma H.K. (2014). Review: Coriander (Coriandrum sativum L.): processing, nutritional and functional aspects. African Journal of Plant Science. 8: 25-33.
  3. Bilgrimi KS, Rizvi MA (1991) Fungi of India-Lists and references. Today and Tomorrow’s Printers and Publishers, New Delhi p: 798.
  4. Chandra S., Kumar P. and Gupta A.B. (2016). Evaluation of fungicides and botanicals in vitro against sheath blight, Rhizoctonia solani in rice (Oryza sativa L.). Plant Archives 16:    167-170.
  5. Dabbas M.R., Singh D.P. and Prakash H.G.(2009). Management of stem gall of coriander through IDM practice. International Journal of Plant Protection 2:255-257.
  6. Gomez K.A. and Gomez A.A. (1983). Statistical Procedures for Agricultural Research. John Wiley and Sons Inc., New York, pp. 357-427.
  7. Govindaraju C. and Somasekhara Y.M. (2016). Management of blast (Pyricularia grisea) of paddy through botanicals and organic products. International Journal of Agricultural Science and Research. 6: 321-326.
  8. Malhotra S.K., Kakani R.K., Sharma Y.K. and Singh D.K. (2016). Ajmer coriander-1(NRCSS, ACR-1) resistant to stem gal disease- an innovative farming technology. Indian Journal of Arecanut, Spices and Medicinal Plants 18:1-7.
  9. Mima K., Kothikar R.B., Patil C.U. (2009). Fungicides and botanicals for managing foliar diseases of turmeric. Journal of Plant Disease Sciences. 4: 68-71.
  10. Mishra P.K., Saha S., Rai A.B. and Rai M. (2009). Antifungal effect of neemazal a commercial product of neem (Azadirachta indica). Journal of Vegetation Science. 36: 60-63.
  11. Mondal N.K., Mojumdar A., Chatterje S.K., Banerjee A., Datta J.K. and Gupta S. (2009). Antifungal activities and chemical characterization of neem leaf extracts on the growth of some selected fungal species in vitro culture medium. Journal of Applied Sciences and Environ-    mental Management. 13: 49–53.
  12. Mukhopadhyay A.N. and Pavgi M.S.(1971). Environment in relation infection of coriander by Protomyces macrosporus Unger. Annals of the Phytopathological Society of Japan 37: 215-219.
  13. Mulla M.S. and Su T.(1999). Activity and biological effects of neem products against arthropods of medical and veterinary importance. Journal of the American Mosquito Control Association. 15:133–152.
  14. Raghavan S (2000). Hand book of spices; Seasoning and flavouring. Ambica Book Agency Jaipur, India p: 457.
  15. Sahib N.G., Anwar F., Gilani A.H., Hamid A.A., Saari A. (2012). Coriander (Coriandrum sativum L.): A potential source of high-value components for functional foods and nutraceuticals- A Review. J Phytother Res 27: 25-33.
  16. Saxena, R.P., Dixit, S., Pandey, V.P. and Singh, V.K. (2002). Germplasm screening for stem gall disease in coriander. In : National Symposium on integrated management of plant disease of mid Eastern India with a cropping system perspective, held at NDUAT, Faizabad during 5-7 December.
  17. Singh U.P. and Prithviraj B. (1997). Neemazal, a product of neem (Azadirachta indica), induces resistance in pea (Pisum sativum) against Erysiphe pisi. Physiological and Molecular Plant Pathology. 51:181–194.
  18. Tripathi A.K. (2003). Effect of sowing dates and weather parameters on stem gall disease of coriander. Indian Phytopathology. 56: 191-193.
  19. Vincent J.M. (1947). Distortion of fungal hyphae in the presence of certain inhibitors. Nature. 150: 850p.
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    Research Article

    volume 35 issue 1-2 (march-june 2020) : 33-38,   Doi: 10.18805/BKAP199

    In-vitro evaluation of six bio-product against coriander stem gall disease

    M
    Munish Leharwan
    M
    Meenu Gupta
    S
    Sanjeev Leharwan
    1<div style="text-align: justify;">Department of Plant Pathology, Yashwant Singh Parmar University of Horticulture and Forestry,&nbsp;Nauni-173 230, Solan, Himachal Pradesh.</div>

    • Submitted22-05-2020|

    • Accepted18-07-2020|

    • First Online 10-09-2020|

    • doi 10.18805/BKAP199

    Cite article:- Leharwan Munish, Gupta Meenu, Leharwan Sanjeev (2020). In-vitro evaluation of six bio-product against coriander stem gall disease. Bhartiya Krishi Anusandhan Patrika. 35(1): 33-38. doi: 10.18805/BKAP199.

    ABSTRACT

    Stem gall of coriander caused by Protomyces macrosporus Unger. is a highly devastating seed and soil borne pathogen in coriander and has emerged as one of the major disease causing substantial quantitative and qualitative losses. In the present studies, efficacy of six bio-products, namely, Neemazal, cow urine, butter milk, fresh milk, beejamrit and jeevamrit was screened against mycelial growth inhibition of P. macrosporus under in vitro conditions. Extracts of these bio-products were evaluated at three different concentrations (i.e 10, 20 and 30%) by poison food technique. Results revealed that that among the various bio-products evaluated against test pathogen, irrespective of concentration tested, all the bio-products resulted in significant inhibition in mycelial growth of test pathogen with neemazal giving the complete inhibition [100.00 % (90.00)] followed by fresh milk [30.66 % (28.44)] and butter milk [26.55 % (25.99)], though these treatments were statistically different from one another. Different concentrations (10 to 30 %) of bio-products, irrespective of the treatments showed that 30 per cent concentration of all bio-products was most efficacious in inhibiting the mycelial growth of the test pathogen giving 69.95 per cent (36.77) growth inhibition. This was followed 20 and 10 per cent concentration of bio-products resulting in 55.58 per cent (32.49) and 45.53 per cent (16.30) growth inhibition, respectively. The result regarding evaluation of bio-products under in vitro conditions against the P. macrosporus showed that Neemazal treatment was most effective at all concentrations and completely inhibited the test fungus followed by 30 per cent fresh milk resulting in the mycelial growth inhibition of 51.60 per cent.

    REFERENCES

    1. Bhat N.M. and Srivastava L.S. (2003). Evaluation of some fungicides and neem formulations against six soil-borne pathogens and three Trichoderma spp. In vitro. Plant Disease Research. 18:56-59.
    2. Bhat S., Kaushal P., Kaur M. and Sharma H.K. (2014). Review: Coriander (Coriandrum sativum L.): processing, nutritional and functional aspects. African Journal of Plant Science. 8: 25-33.
    3. Bilgrimi KS, Rizvi MA (1991) Fungi of India-Lists and references. Today and Tomorrow’s Printers and Publishers, New Delhi p: 798.
    4. Chandra S., Kumar P. and Gupta A.B. (2016). Evaluation of fungicides and botanicals in vitro against sheath blight, Rhizoctonia solani in rice (Oryza sativa L.). Plant Archives 16:    167-170.
    5. Dabbas M.R., Singh D.P. and Prakash H.G.(2009). Management of stem gall of coriander through IDM practice. International Journal of Plant Protection 2:255-257.
    6. Gomez K.A. and Gomez A.A. (1983). Statistical Procedures for Agricultural Research. John Wiley and Sons Inc., New York, pp. 357-427.
    7. Govindaraju C. and Somasekhara Y.M. (2016). Management of blast (Pyricularia grisea) of paddy through botanicals and organic products. International Journal of Agricultural Science and Research. 6: 321-326.
    8. Malhotra S.K., Kakani R.K., Sharma Y.K. and Singh D.K. (2016). Ajmer coriander-1(NRCSS, ACR-1) resistant to stem gal disease- an innovative farming technology. Indian Journal of Arecanut, Spices and Medicinal Plants 18:1-7.
    9. Mima K., Kothikar R.B., Patil C.U. (2009). Fungicides and botanicals for managing foliar diseases of turmeric. Journal of Plant Disease Sciences. 4: 68-71.
    10. Mishra P.K., Saha S., Rai A.B. and Rai M. (2009). Antifungal effect of neemazal a commercial product of neem (Azadirachta indica). Journal of Vegetation Science. 36: 60-63.
    11. Mondal N.K., Mojumdar A., Chatterje S.K., Banerjee A., Datta J.K. and Gupta S. (2009). Antifungal activities and chemical characterization of neem leaf extracts on the growth of some selected fungal species in vitro culture medium. Journal of Applied Sciences and Environ-    mental Management. 13: 49–53.
    12. Mukhopadhyay A.N. and Pavgi M.S.(1971). Environment in relation infection of coriander by Protomyces macrosporus Unger. Annals of the Phytopathological Society of Japan 37: 215-219.
    13. Mulla M.S. and Su T.(1999). Activity and biological effects of neem products against arthropods of medical and veterinary importance. Journal of the American Mosquito Control Association. 15:133–152.
    14. Raghavan S (2000). Hand book of spices; Seasoning and flavouring. Ambica Book Agency Jaipur, India p: 457.
    15. Sahib N.G., Anwar F., Gilani A.H., Hamid A.A., Saari A. (2012). Coriander (Coriandrum sativum L.): A potential source of high-value components for functional foods and nutraceuticals- A Review. J Phytother Res 27: 25-33.
    16. Saxena, R.P., Dixit, S., Pandey, V.P. and Singh, V.K. (2002). Germplasm screening for stem gall disease in coriander. In : National Symposium on integrated management of plant disease of mid Eastern India with a cropping system perspective, held at NDUAT, Faizabad during 5-7 December.
    17. Singh U.P. and Prithviraj B. (1997). Neemazal, a product of neem (Azadirachta indica), induces resistance in pea (Pisum sativum) against Erysiphe pisi. Physiological and Molecular Plant Pathology. 51:181–194.
    18. Tripathi A.K. (2003). Effect of sowing dates and weather parameters on stem gall disease of coriander. Indian Phytopathology. 56: 191-193.
    19. Vincent J.M. (1947). Distortion of fungal hyphae in the presence of certain inhibitors. Nature. 150: 850p.
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