Bhartiya Krishi Anusandhan Patrika, volume 32 issue 1 (march 2017) : 44-48

Identification of genetic polymorphism in fecundity gene in Kendrapada sheep 

Basanti Jyotsana, Amar Singh Meena, Rajiv Kumar, Rajni Kumari, L.L.L. Prince, Satish Kumar
1<p>Animal Biotechnology Section,&nbsp;ICAR-Central Sheep and Wool Research Institute, Avikanagar, Malpura-304501 (Rajasthan)<br /> &nbsp;</p>
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Cite article:- Jyotsana Basanti, Meena Singh Amar, Kumar Rajiv, Kumari Rajni, Prince L.L.L., Kumar Satish (NaN). Identification of genetic polymorphism in fecundity gene in Kendrapada sheep . Bhartiya Krishi Anusandhan Patrika. 32(1): 44-48. doi: undefined.

    FecB gene is mainly responsible for prolificacy in sheep. FecB gene in various countries in the world utilizing for increasing the economic profit of sheep farming using as molecular marker for selection of animals.This study has been conducted in Kendrapada sheep breed in ICAR-Central Sheep and Wool Research Institite farm at Avikanagar. In this study, the genetic polymorphism of the fecundity gene has been detected. Genomic DNA of 120 sheep has been isolated and FecB gene amplified by gene specific primer. After the PCR-RFLP analysis, we found that, Kendrapada sheep have been reported all three genotypes with genotyping frequency of BB, ++ and B+ as 0.40, 0.13 and 0.47 respectively. The allelic frequency have been found for B and + alleles as 0.63 and 0.37 respectively. Kendrapada sheep breed will be utilizing in marker assisted breeding programme in non-prolific sheep breed. 

  1. Anonymous (2016). New breeds of Indigenous Livestock and Poultry. Indian Journal of Animal Science, 86 (10): 1113-1227.

  2. Davis, G.H., Galloway, S.M., Ross, I.K., Gregan, S.C., Ward, J., Nimbkar, B.V., Ghalsasi, P.M., Nimbkar, C., Douglas Gray, G., Subandriyo, Inounu, I., Tiesnamurti, B., Martyniuk, E., Eythorsdottir, E., Mulsant, P., Lecer, F., Hanrahan, J.P., Eric Bradford, G., Wilson, T (2002). DNA tests in prolific sheep from eight countries provide new evidence on origin of the Booroola (FecB) mutation. Biol. Reprod. 66: 1869–1874.

  3. Davis, GH., (2004). Fecundity genes in sheep. Anim. Reprod. Sci. 82–83:247–253.

  4. Kumar S., Mishra AK., Kolte AP., Dash SK and Karim SA (2008). Screening for Booroola (FecB) and Galway (FecX G) mutations in Indian sheep. Small Ruminant Research 80 (1), 57-61.

  5. Wilson T, Wu XY, Juengel JL, Ross IK, Lumsden JM, Lord EA, Dodds KG, Walling GA, McEwan JC, O’Connell AR et al. 2001 Highly prolific Booroola sheep have a mutation in the intracellular kinase domain of bone morphogenetic protein IB receptor (ALK-6) that is expressed in both oocytes and granulosa cells. Biology of Reproduction 64 1225–1235.

  6. Mulsant, P. ; Lecerf, F. ; Fabre, S. ; Schibler, L. ; Monget, P. ; Lanneluc, I. ; Pisselet, C. ; Riquet, J. ; Monniaux, D. ; Callebaut, I. (2001). Mutation in bone morphogenetic protein receptor-IB is associated with increased ovulation rate in Booroola Merino ewes. PNAS 98 5104–5109.

  7. Fahmy MH, Davis GH (1996). Breeds with newly discovered genes for prolificacy. In: Fahmy MH (ed.), Prolific Sheep. Wallingford, UK: CAB International; 1996: 174–177.

  8. Meena AS, Bhatt RS, Sahoo A and Kumar S (2016). Polymorphism of the exon 3 of leptin gene in malpura sheep. Indian Journal of Animal Research. DOI: 10.18805/ijar.v0i0f.3783. 

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