Chief EditorYashpal Singh Malik
Print ISSN 0303-3821
Online ISSN 0976-4631
NAAS Rating 3.07
Microsatellites for coriander crop: A cross species amplification
First Online |
Development of microsatellites (Simple Sequence Repeats) is an expensive and time consuming process for new crop like coriander, but this can be easily achieved by transferring the microsatellite loci from the same family/genus/species which is a cost-effective approach for development of microsatellites in lesser studied crops like coriander. For cross-species investigation presently carrot and celery microsatellites are available on the public domain. We examined the transferability of hundred carrot (Daucuscarota) microsatellite loci to coriander (Coriandrum sativum L.). Thirty nine percent primer (39/100) amplified for coriander. Out of 39 microsatellites loci, 35 primers were monomorphic and specific to carrot primers, showing approximately the same size of microsatellites loci as reported for carrot. Four SSRs were polymorphic; seven primer pairs were non-specific which were deviating for band size from the source of microsatellites. These microsatellites markers should provide a powerful tool for coriander breeding and genetics.
- Aruna G and Baskaran V (2010) Comparative study on the levels of carotenoids lutein, zeaxanthin and b-carotene in Indian spices of nutritional and medicinal importance. Food Chemistry 123:404–409
- Barbara T, Palma-Silva C, Paggi GM, Bered F, Fay MF, Lexer C (2007) Cross-Species transfer of nuclear microsatellite markers: Potential and limitations. Mol Ecol 16:3759-3767
- Bushar LM, Maliga M, and Reinert HK (2001) Cross- species amplification of Crotalus horridus microsatellites and their application in phylogenetic analysis. J Herpetol 35:532-537
- Cavagnaro PF, Chung SM, Manin S, Yildiz M, Ali A, Alessandro MS, Iorizzo M, Senalik DA and Simon PW (2011) Microsatellite isolation and marker development in carrot - genomic distribution, linkage mapping, genetic diversity analysis and marker transferability across Apiaceae. BMC Genomics 12: 386
- Gupta PK and Varshney RK (2000) The development and use of microsatellite markers for genetic analysis and plant breeding with special emphasis on bread wheat. Euphytica 113:163-185
- Kantety RV, Rota ML, Matthews DW, Sorrells ME (2002) Data mining for simple sequence repeats in expressed sequence tags from barley, maize, rice, sorghum and wheat. Plant Mol Biol 48:501-510
- Kuleung C, Baenziger PS, and Dweikat I (2004) Transferability of SSR markers among wheat, rye, and triticale. Theor Appl Genet 108:1147- 1150
- Peakall R, Gilmore S, Keys W, Morgante M, Rafalski A (1998) Cross-Species amplification of Soyebean (Glycine max) simple sequence repeats (SSRs) within the genus and other legume genera: Implication for the transferability of SSRs in plants. Mol Biol Evol 15:1275-1287
- Roder MS, Plaschke J, Konig SU, Borner A, Sorrells ME, Tanksley SD, Ganal MW (1995) Abundance, variability and chromosomal location of micro-satellites in wheat. Mol Genet 46: 327-333
- Schnell RJ, Olano CT, Quintanilla WE and Meerow AW (2005) Isolation and characterization of 15 microsatellite loci from mango (Mangifera indica L.) and cross-species amplification in closely related taxa. Mol Ecol Notes 5: 626-627
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.