Present study carried out in and around the sangamner area. A total of 34 cases presented for the treatment of repeat breeding during the period of Eight months. The animals were divided into six groups consisting total 36 animals. In group I (11 cross bread cow)- Treated with Ciprofloxacin and Tinidazole combination (20 ml in each horn) 40-50 ml intra-uterine daily for 2 days. Group II (9 cross bread cow)- treated with Gentamicin sulphate, does - 10 ml Gentamicin + 30 ml distilled water (20 ml in each horn) intrauterine for 2 days during estrous period. Group III (9 cross bread cow) - Treated with Tetracycline Hydrochloride 10 ml + 30 ml distilled water (20 ml in each horn) intrauterine for 2 days during estrous period. In Group IV (7 cross bread cow)- Treated with 40 ml distilled water (20 ml in each horn)  intrauterine for 2 days during estrous period. The pregnancy diagnosis was carried out at 60 days after. A.I. by per-rectal examination and the clinical efficacy of different intrauterine preparations were determined on the basis of conception rate among the groups. It is concluded that Ciprofloxacin and Tinidazole may be used as the most effective drug for the treatment of repeat breeding in animals at field condition as compared to other intrauterine preparations.