Agricultural Reviews

  • Chief EditorPradeep K. Sharma

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Agricultural Reviews, volume 40 issue 4 (december 2019) : 249-260

Importance of Bioactive Compounds Present in Plant Products and Their Extraction – A Review

Amardeep Kumar, Faizan Ahmad, Sadaf Zaidi
1Department of Food Engineering and Technology, School of Engineering, Tezpur University, Napaam-784 028, Assam, India.
Cite article:- Kumar Amardeep, Ahmad Faizan, Zaidi Sadaf (2019). Importance of Bioactive Compounds Present in Plant Products and Their Extraction – A Review. Agricultural Reviews. 40(4): 249-260. doi: 10.18805/ag.R-1926.
India is one of the largest producers of fruits and vegetables in the world. These are rich sources of bioactive compounds which provide health benefits and also possess antioxidant therapeutic value. A large portion of fruits and vegetables goes unutilized in the form of pulp and peels after the extraction of juice in food processing industries. This contains a large number of bioactive compounds. The compounds like phenolic compounds, flavonoids and carotenoids are ubiquitously present in fruits, vegetables and their by-products. These compounds have antioxidant, antimicrobial, anticancer, antiviral, antitumor and many more activities to a greater or lesser extent. This review focuses on the bioactive compounds present in fruits, vegetables and their by-products along with their classification and importance in day to day life. A further aim of this review is to discuss various techniques employed in the extraction of bioactive compounds from plant products. The antioxidant activity of various fruits and vegetables based on DPPH radical scavenging methods has also been reported in this work. 
  1. Abdel-Aty AM, Salama WH, Hamed MB, Fahmy AS, Mohamed SA (2018). Phenolic-antioxidant capacity of mango seed kernels: therapeutic effect against viper venoms Revista Brasileira de Farmacognosia. 28: 594-601 doi:
  2. Ahmad F. and Khan S.T. (2019). Potential industrial use of componds from By-products of fruits and vegetables. In: Malik A., Erginkaya Z., Erten H., (eds) Health and Safty Aspects of Food Processing Technologies. Springer, Cham. 
  3. AIMPLAS, Instituto Tecnológico del plástico (2012). Extractos de plantas para desarrollar envases quealarguen la vida de alimentos frescos.
  4. Ajila, C.M., Naidu, K.A., Bhat, S.G., Prasada Rao, U.J.S. (2007). Bioactive compounds and antioxidant potential of mango peel extract. Food Chemistry, 105: 982-988.
  5. Alasalvar, C., Grigor, J.M., Zhang, D., Quantick, P.C., Shahidi, F. (2001). Comparision of volatiles, phenolics, sugars, antioxidant vitamins and sensory quality of different colored carrot varieties. Journal of Agricultural and Food Chemistry. 49: 1410-1416.
  6. Alupului, A., Calinescu, I., Lavric, V. (2012). Microwave extractions of active principles from medicinal plants. U.P.B. Science Bulletin. 74: 129-142.
  7. Azmir, J., Zaidul, I.S.M., Rahman, M. M., Sharif, K.M., Mohamed, A., Sahena, F., Jahurul, M.H.A., Ghafoor, K., Norulaini, N.A.N., Omar, A.K.M. (2013). Techniques for exraction of bioactive compounds from plant materials: A review. Journal of Food Engineering. 117: 426-436.
  8. Bai XL, Yue TL, Yuan YH, Zhang HW (2010) Optimization of microwave-assisted extraction of polyphenols from apple pomace using response surface methodology and HPLC analysis Journal of separation science 33:3751-3758 doi:10.1002/ jssc.201000430
  9. Balasundram, N., Sundram, K. and Samman, S. (2006). Phenolic compounds in plants and agri-industrial by-products: Antioxidant activity, occurrence and potential uses. Food Chemistry. 99: 191-203.
  10. Barba, F.J., Parniakov, O., Pereira, S.A., Wiktor, A., Grimi, N., Boussetta, N., Saraiva, J.A., Raso, J., Martin-Belloso, O., Witrowa-Rajchert, D., Lebovka, N., Vorobiev, E. (2015). Current applications and new oppoutunities for the use of pulsed electric fields in food science and industry. Food Research International. 77: 773-798.
  11. Benelli P, Riehl C, Smania Junior A, F. A. Smânia E, Ferreira S (2010). Bioactive extracts of orange ( Citrus sinensis L. Osbeck) pomace obtained by SFE and low pressure techniques: Mathematical modeling and extract composition vol 55. doi:10.1016/j.supflu.2010.08.015.
  12. Bonilla J, Talón E, Atarés L, Vargas M, Chiralt A (2013). Effect of the incorporation of antioxidants on physicochemical and antioxidant properties of wheat starch–chitosan films Journal of Food Engineering. 118: 271-278. doi:
  13. Borneo, R., Leon, A.E., Aguirre, A., Ribotta, P., Cantero, J.J. (2009). Antioxidant capacity of medicinal plants from the Province of Codoba (Argentina) and their in vitro testing in a model food system. Food Chemistry. 112: 664-670.
  14. Bouzrara, H., Vorobiev, E. (2003). Solid-liquid expression of cellular materials enhanced by pulsed electric field. Chemical Engineering and Processing: Process Intensification. 42: 249-257.
  15. Bravo, L. (1998). Polyphenols: chemistry, dietary sources, metabolism and nutritional significance. Nutrition Reviews. 56: 317-333.
  16. Burnaz, N.A., Kucuk, M., Akar, Z. (2017). An on-line HPLC system for detection of antioxidant compounds in some plant extracts by comparing three different methods. Journal of Chromatography B. 1052: 66-72.
  17. Cagri-Mehmetoglu A (2015). Antimicrobial Edible Films. In. 
  18. Cai, Y.Z., Luo, Q., Sun. M., Corke, H. (2004). Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Science. 74: 2157-2184.
  19. Cardenas-Toro FP (2015). Pressurized liquid extraction and low-    pressure solvent extraction of carotenoids from pressed palm fiber: Experimental and economical evaluation Food and bioproducts processing v. 94: pp. 90-100-2015 v.2094 doi:10.1016/j.fbp.2015.01.006
  20. Chan, C.H., Yusoff, R., Ngoh, G.C., Kung, F.W.L. (2011). Microwave-assisted extractions of active ingredients from plants. Journal of Chromatography A. 1218: 6213-6225.
  21. Chemat, F., Tomao, V., Virot, M. (2008). Handbook of food analysis instruments. Ultrasound assisted extraction in food analysis. CRC Press, 85-94.
  22. Chemat, F., Zill-e-Huma, Khan, M.K. (2011). Applications of ultrasound in food technology: Processing, preservation and extraction. UltrasonicsSonochemistry. 18: 813-835.
  23. Chu, Y.F., Sun, J., Wu, X., Liu, R.H. (2002). Antioxidant and antiproliferative activities of common vegetables. Journal of Agricultural and Food Chemistry. 50: 6910-6916.
  24. Corrales M, Toepfl S, Butz P, Knorr D, Tauscher B (2008). Extraction of anthocyanins from grape by-product assisted by ultrasonics, high hydrostatic pressure or pulsed electric fields: A comparison vol 9. doi:10.1016/j.ifset.2007.06.002
  25. Croteau, R., Kutchan, T. M., Lewis, N. G. (2000). Natural products (secondary metabolites). Biochemistry and Molecular Biology of Plants. American Society of Plant Physiologists, New Jersey, USA. 1250 1318.
  26. Da Silva, R.P.F.F, Rocha-Santos, T.A.P., Duarte, A.C. (2016). Supercritical fluid extraction of bioactive compounds. TrAC Trends in Analytical Chemistry. 76: 40-51.
  27. Degrou, A., George, S., Renard, C.M.G.C., Page, D. (2013). Physicochemical parameters that influence carotenoids bio accessibility from a tomato juice. Food Chemistry. 136: 435-441.
  28. Deng, G.F., Lin, X., Xu, X.R., Gao, L.L., Xie, J.F., Li, H.B. (2013). Antioxidant capacities and total phenolic content of 56 vegetables. Journal of Functional Foods. 5: 260-266.
  29. Díaz-Reinoso B, Moure A, Domínguez H, Parajó JC (2006). Supercritical CO2 Extraction and Purification of Compounds with Antioxidant Activity Journal of Agricultural and Food Chemistry. 54: 2441-2469 doi:10.1021/jf052858j
  30. Eca K, Sartori T, Menegalli FC (2014) Films and edible coatings containing antioxidants—A review Brazilian Journal of Food Technology 17:98-112 doi:10.1590/bjft.2014.017
  31. Faizan A, Kumar A (2018). Optimization of the ultrasonic assisted extraction process to obtain phenolic compounds from pomegranate (Punica granatum) peels using response surface methodology. Int. J. Agric. Sci. 10: 7581.
  32. Guo, X., Shang, X., Zhou, X., Zhao, B., Zhang, J. (2017). Ultrasound-    assisted extraction of polysaccharides from Rhododendron aganniphum: Antioxidant activity and rheological properties. UltrasonicsSonochemistry. 38: 246-255.
  33. Hasbay Ý, Ý. Çetin H, Yener E, Bayindirli A (2007). Subcritical (carbon dioxide + ethanol) extraction of polyphenols from apple and peach pomaces and determination of the antioxidant activities of the extracts vol 43. doi:10.1016/    j.supflu.2007.04.012
  34. Hawthorne, S.B., Yang, Y., Miller, D.J. (1994). Extraction of organic pollutants from environmental solids with sub and supercritical water. Analytical Chemistry. 66: 2912-2920.
  35. Ignat, I., Volf, I., Popa, V. I. (2011). A critical review of methods for characterisation of polyphenolic compounds in fruits and vegetables. Food Chemistry. 126: 1821-1835.
  36. Juneja VK, Dwivedi HP, Yan X (2012). Novel Natural Food Antimicrobials 3:381-403 doi:10.1146/annurev-food-022811-101241
  37. Kammerer, D., Carle, R., Schieber, A. (2004). Quantification of anthocyanins in black carrot extracts (Dacuscarota ssp. Sativus var. atrorubensAlef.) and evaluation of their color properties. European Food Research and Technology. 219: 479-486.
  38. Kanatt S, Chander R, Sharma A (2010). Antioxidant and antimicrobial activity of pomegranate peel extract improves the shelf life of chicken products vol 45. doi:10.1111/j.1365-2621.2009.02124.x
  39. Kanjilal T, Babu S, Biswas K, Bhattacharjee C, Datta S (2014). Application of mango seed integuments as bio-adsorbent in lead removal from industrial effluent vol. 56. doi:10.1080/ 19443994.2014.950999
  40. Kaur, C., Kapoor, H.C. (2002). Anti-oxidant activity and total phenolic content of some Asian vegetables. International Journal of Food Science and Technology. 37: 153-161.
  41. Khan MK, Abert-Vian M, Fabiano-Tixier A-S, Dangles O, Chemat F (2010). Ultrasound-assisted extraction of polyphenols (flavanone glycosides) from orange (Citrus sinensis L.) peel Food chemistry 2010 v.119 no.2: pp. 851-858. doi:10.1016/j.foodchem.2009.08.046
  42. Kittiphoom S (2012) Utilization of mango seed vol 19. 
  43. Kodagoda G, Marapana U (2017). Utilization of fruit processing by-products for industrial applications: A review vol. 24. 
  44. Kris-Etherton PM et al. (2002). Bioactive compounds in foods: their role in the prevention of cardiovascular disease and cancer The American journal of medicine 113 Suppl 9B:71s-88s doi:10.1016/s0002-9343(01)00995-0
  45. Krishnaswamy K, Orsat V, Gariépy Y, Thangavel K (2013). Optimization of Microwave-Assisted Extraction of Phenolic Antioxidants from Grape Seeds (Vitis vinifera) Food and Bioprocess Technology. 6: 441-455 doi:10.1007/s11947-012-0800-2.
  46. Lang, Q., Wai, C.M. (2001). Supercritical fluid extraction in herbal and natural product studies – a practical review. Talanta. 53: 771-782.
  47. Li, Y., Guo, C., Yang, J., Wei, J., Xu, J., Cheng, S. (2006). Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract. Food Chemistry. 96: 254-260.
  48. Liazid A, Guerrero RF, Cantos E, Palma M, Barroso CG (2010). Microwave assisted extraction of anthocyanins from grape skins Food Chemistry. 124: 1238-1243 doi:10.1016/ j.foodchem.2010.07.053
  49. López-Rubio A, Gavara R, Lagaron JM (2006). Bioactive packaging: Turning foods into healthier foods through biomaterials Trends in Food Science & Technology 17:567-575 doi:10.1016/j.tifs.2006.04.012
  50. Luchese CL, Garrido T, Spada JC, Tessaro IC, de la Caba K (2018). Development and characterization of cassava starch films incorporated with blueberry pomace International Journal of Biological Macromolecules. 106:834-839 doi:https://
  51. Luque de Castro, M.D., Garcia-Ayuso, L.E. (1998). Soxhlet extraction of solid materials: an outdated technique with a promising innovative future. AnalyticaChimicaActa. 369: 1-10.
  52. Luximon-Ramma, A., Bahorun, T., Crozier, A. (2003). Antioxidant actions and phenolic and vitamin C contents of common Mauritian exotic fruits. Journal of the Science of Food and Agriculture. 83: 496-502.
  53. M. Farías-Campomanes A, Rostagno M, Meireles MA (2013). Production of polyphenol extracts from grape bagasse using supercritical fluids: Yield, extract composition and economic evaluation vol 77. doi:10.1016/j.supflu.2013.02.006
  54. Machado, A.P.D.F., Reategui, J.L.P., Barbero, G.F., Martinez, J. (2015). Pressurized liquid extraction of bioactive compounds from blackberry (Rubusfruticosus L.) residues: a comparison with conventional methods. Food Research International. 77: 675-683.
  55. Meltzer, H. M. (2010). Bioactive compounds through food, nutraceuticals or pills. A brief review on bioactive compounds in plants. The Norwegian Academy of Science and Letters. 205-222.
  56. Michel T, Destandau E, Le Floch G, Lucchesi M, Elfakir C (2012) Antimicrobial, antioxidant and phytochemical investigations of sea buckthorn (Hippophaë rhamnoides L.) leaf, stem, root and seed vol 131. doi:10.1016/j.foodchem.2011.09.029
  57. Miliauskas, G., Venskutonis, P.R., Beek, T.A., (2004). Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chemistry. 85: 231-237.
  58. Monrad, J.K., Howard, L.R., King, J.W., Srinivas, K., Mauromoustakos, A. (2010). Subcritical solvent extraction of anthocyanins from dried red grape pomace. Journal of Agricultural and Food Chemistry. 58: 2862-2868.
  59. Moo-Huchin, V.M., Estrada-Mota, I., Estrada-Leon, R., Cuevas-    Glory, L., Ortiz-Vazquez, E., Vargas, M.L.V., Betancur-    Ancona, D., Sauri-Duch, E. (2014). Determination of some physicochemical characteristics, bioactive compounds and antioxidant activity of tropical fruits from Yucatan, Mexico. Food Chemistry. 152: 508-515.
  60. Mustafa, A., Turner, C. (2011). Pressurized liquid extraction as a green approach in food and herbal plants extraction: A review. AnalyticaChimicaActa. 703: 8-18.
  61. Naczk, M., &Shahidi, F. (2006). Phenolics in cereals, fruits and vegetables: occurance, extraction and analysis. Journal of Pharmaceutical and Biomedical Analysis. 41: 1523-1542.
  62. Nieto, A., Borrull, F., Pocurull, E., Marce, R.M. (2010). Pressurized liquid extraction: a useful technique to extract pharmaceuticals and personalcare products from sewage sludge. TrAc Trends in Analytical Chemistry. 29: 752-764.
  63. Pan, X., Niu, G., Liu, H. (2003). Microwave-assisted extraction of tea polyphenols and tea caffeine from green tea leaves. Chemical Engineering and Processing. 42: 129-133.
  64. Pastor C, Sánchez-González L, Marcilla A, Chiralt A, Cháfer M, González-Martínez C (2011). Quality and safety of table grapes coated with hydroxypropylmethylcellulose edible coatings containing propolis extract Postharvest Biology and Technology 60: 64-70 doi:    j.postharvbio.2010.11.003
  65. Pereira A, Maraschin M (2015). Banana (Musa spp) from peel to pulp: ethnopharmacology, source of bioactive compounds and its relevance for human health. Journal of ethnopharmacology. 160: 149-163 doi:10.1016/    j.jep.2014.11.008
  66. Prado JM et al. (2012). Supercritical fluid extraction of grape seed: Process scale-up, extract chemical composition and economic evaluation Journal of Food Engineering. 109: 249-257 doi:
  67. Prakash Maran J, Sivakumar V, Thirugnanasambandham K, Sridhar R (2013). Optimization of microwave assisted extraction of pectin from orange peel Carbohydrate Polymers. 97: 703-709 doi:
  68. Quagliariello, V., Iaffaioli, R.V., Falcone, M., Ferrari, G., Pataro, G., Donsi, F. (2016). Effect of pulsed electric fields – assisted extraction on anti-inflammatory and cytotoxic activity of brown rice bioactive compounds. Food Research International. 87: 115-124.
  69. Radusin T, Kevrešan Ž, Mastiloviæ J, Novakoviæ A, Janiæ Hajnal E (2013). Influence of different packaging solutions on qualitative and quantitative properties of fresh tomato variety Izmir during storage at market conditions Food and Feed Researc.h 40: 85-92
  70. Redondo, D., Venturini, M.E., Luengo, E., Raso, J., Arias, E. (2018). Pulsed electric fields as a green technology for the extraction of bioactive compounds from thinned peach by-products. Innovative Food Science & Emerging Technologies. 45: 335-343.
  71. Ribeiro da Silva, L.M., Teixeira de Figueiredo, E.A., Ricardo, N.M.P.S., Vieira, I.G.P., Wilane de Figueiredo, R., Brasil, I.M., Gomes, C.L. (2014). Quantification of bioactive compounds in pulps and by-products of tropical fruits from Brazil. Food Chemistry. 143: 398-404.
  72. Sass-Kiss, A., Kiss,J., Milotay, P., Kerek, M.M., Toth-Markus, M. (2005). Differences in anthocyanin and carotenoid content of fruits and vegetables. Food Research International. 38: 1023-1029.
  73. Sharif, K.M., Rahman, M.M., Azmir, J., Mohamed, A., Jahurul, M.H.A., Sahena, F., Zaidul, I.S.M. (2014). Experimental design of supercritical fluid extraction – A review. Journal of Food Engineering. 124: 105-116.
  74. Silva, L.V., Nelson, D.L., Drummond, M.F.B., Dufosse, L., Gloria, M.B.A. (2005). Comparison of hydrodistillation methods for the deodorization of turmeric. Food Research International. 38: 1087-1096.
  75. Silva-Weiss A, Ihl M, Sobral PJdA, Gómez-Guillén MC, Bifani V (2013). Natural additives in bioactive edible films and coatings: functionality and applications in foods. Food Engineering Reviews 5:200-216 doi:10.1007/s12393-    013-9072-5
  76. Soria, A. C., Villamiel, M. (2010). Effect of ultrasound on the technological properties and bioactivity of food: A review. Trends in Food Science and Technology. 21: 323–331.
  77. Sreeramulu, D., Raghunath, M. (2010). Antioxidant activity and phenolic content of roots, tubers and vegetables commonly consumed in India. Food Research International. 43: 1017-1020.
  78. Sun, J., Chu, Y.F., Wu, X., Liu, R.H. (2002). Antioxidant and antiproliferative activities of common fruits. Journal of Agricultural and Food Chemistry. 50: 7449-7454.
  79. Temelli, F., Guclu-Ustundag, O. (2005). Supercritical technologies for further processing of edible oils. Bailey’s Industrial Oil and Fat Products. John Wiley & Sons, Inc.
  80. Vankar, P.S. (2004). Essential oils and fragrances from natural sources. Resonance. 9: 30-41.
  81. Vasco, C., Ruales, J., Kamal-Eldin, A. (2008). Total phenolics compounds and antioxidant capacities of major fruits from Ecuador. Food Chemistry. 111: 816-823.
  82. Vázquez, M. F. B., Comini, L. R., Martini, R. E., Montoya, S. C. N., Bottini, S., Cabrera, J. L. (2014). Comparisons between conventional, ultrasound-assisted and microwave-assisted methods for extraction of anthraquinones from Heterophyllaeapustulata Hook f. (Rubiaceae). Ultrasonics Sonochemistry. 21: 478-484.
  83. Virot M, Tomao V, Le Bourvellec C, Renard CM, Chemat F (2010). Towards the industrial production of antioxidants from food processing by-products with ultrasound-assisted extraction. Ultrason Sonochem. 17: 1066-1074 doi:10.1016/j.ultsonch.2009.10.015
  84. Wang S, Chen F, Wu J, Wang Z, Liao X, Hu X (2007). Optimization of pectin extraction assisted by microwave from apple pomace using response surface methodology vol 78. doi:10.1016/j.jfoodeng.2005.11.008
  85. Zotchev SB (2013). Chapter Eleven - Alkaloids from Marine Bacteria. In: Giglioli-Guivarc’h N (ed) Advances in Botanical Research, vol 68. Academic Press, pp 301-333. doi:    10.1016/B978-0-12-408061-4.00011-0.

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